Screening of Lipid Production Capacities of Bacillus sp. Strains Isolated  from Soil and Lipid Staining with Different Staining Techniques

Elif Demirkan; İrem Yıldırım

Makale detayları

Screening of Lipid Production Capacities of Bacillus sp. Strains Isolated from Soil and Lipid Staining with Different Staining Techniques

Yazarlar:

Elif Demirkan | İrem Yıldırım

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Endüstriyel Biyoteknoloji

Dergi:

Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi Vol.23 Issue 5, pp.1094-1102

Belge Tipi:

Makale

Yayın Tarihi:

10/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001302511

pISSN:

2149-3367

Anahtar kelimeler:

Özet:

 Microbial lipids have been attracting more and more attention in recent years as promising raw materials for the production of biodiesel and value-added compounds. In the current economic and environmental situation, finding new renewable sources of lipids will be crucial. Recent research has necessitated the search for new efficient microbial producers with lipid production efficiency. For this purpose,50 Bacillus sp. strains previously isolated from the soil were screened for their lipid production capacity. As a result of the production using a single-cell oil production medium, only two Bacillus spp. strains showed growth. These bacteria were named as Bacillus sp. E40 and Bacillus sp. E226. Bacteria were then characterized in terms of their biomass, lipid yields, and lipid contents. The biomass of Bacillus sp. E40 and E226 bacterial isolates, were 0,28 and 0,22 g.L-1, respectively. The highest lipid content was reached by E226 with 54.5. Bacillus sp. E40 lipid content was determined as 46.4. In addition, both intracellular lipid staining and colony staining demonstrated the presence of lipids. With this study, it was reported for the first time that local Bacillus sp. strains isolated from soil have lipid producing capacity.

 Microbial lipids have been attracting more and more attention in recent years as promising raw materials for the production of biodiesel and value-added compounds. In the current economic and environmental situation, finding new renewable sources of lipids will be crucial. Recent research has necessitated the search for new efficient microbial producers with lipid production efficiency. For this purpose,50 Bacillus sp. strains previously isolated from the soil were screened for their lipid production capacity. As a result of the production using a single-cell oil production medium, only two Bacillus spp. strains showed growth. These bacteria were named as Bacillus sp. E40 and Bacillus sp. E226. Bacteria were then characterized in terms of their biomass, lipid yields, and lipid contents. The biomass of Bacillus sp. E40 and E226 bacterial isolates, were 0,28 and 0,22 g.L-1, respectively. The highest lipid content was reached by E226 with 54.5. Bacillus sp. E40 lipid content was determined as 46.4. In addition, both intracellular lipid staining and colony staining demonstrated the presence of lipids. With this study, it was reported for the first time that local Bacillus sp. strains isolated from soil have lipid producing capacity.

 Microbial lipids have been attracting more and more attention in recent years as promising raw materials for the production of biodiesel and value-added compounds. In the current economic and environmental situation, finding new renewable sources of lipids will be crucial. Recent research has necessitated the search for new efficient microbial producers with lipid production efficiency. For this purpose,50 Bacillus sp. strains previously isolated from the soil were screened for their lipid production capacity. As a result of the production using a single-cell oil production medium, only two Bacillus spp. strains showed growth. These bacteria were named as Bacillus sp. E40 and Bacillus sp. E226. Bacteria were then characterized in terms of their biomass, lipid yields, and lipid contents. The biomass of Bacillus sp. E40 and E226 bacterial isolates, were 0,28 and 0,22 g.L-1, respectively. The highest lipid content was reached by E226 with 54.5. Bacillus sp. E40 lipid content was determined as 46.4. In addition, both intracellular lipid staining and colony staining demonstrated the presence of lipids. With this study, it was reported for the first time that local Bacillus sp. strains isolated from soil have lipid producing capacity.

 Mikrobiyal lipitler, biyodizel ve katma değerli bileşiklerin üretimi için umut verici hammaddeler olarak son yıllarda giderek daha fazla dikkat çekmektedir. Mevcut ekonomik ve çevresel durumda, yeni yenilenebilir lipit kaynakları bulmak çok önemli olacaktır. Son araştırmalar, lipit üretim verimliliğine sahip yeni verimli mikrobiyal üreticilerin araştırılmasını gerekli kılmıştır. Bu amaçla daha önce topraktan izole edilen 50 Bacillus sp. suşu lipid üretme kapasiteleri açısından taranmıştır. Tek hücreli yağ üretimi ortamında yapılan üretim sonucunda bu bakterilerden sadece iki Bacillus sp. suşları üreme göstermiştir. Bu bakterilerden sadece 2 Bacillus sp. suşu tek hücreli yağ üretimi ortamında üremiştir. Bu bakteriler Bacillus sp.E40 ve Bacillus sp. E226 olarak adlandırıldı. Bakteriler daha sonra biyokütleleri, lipid verimleri ve lipid içerikleri açısından karakterize edildi. Bacillus sp. E40 ve E226 bakteri izolatlarının biyokütlesi sırasıyla, 0,28 ve 0,22 g.L-1 idi. En yüksek lipid içeriğine 54.5 ile E226da ulaşılmıştır. Bacillus sp. E40 lipid içeriği 46.4 olarak belirlendi. Ek olarak, lipid varlığı hem hücre içi lipid boyama hem de koloni boyama ile gösterilmiştir Bu çalışma ile topraktan izole edilen yerel Bacillus sp. suşlarının lipit üretme kapasitesine sahip olduğu ilk kez rapor edilmiştir.

Makale detayları

Screening of Lipid Production Capacities of Bacillus sp. Strains Isolated from Soil and Lipid Staining with Different Staining Techniques

Yazarlar:

Elif Demirkan | İrem Yıldırım

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Endüstriyel Biyoteknoloji

Dergi:

Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi Vol.23 Issue 5, pp.1094-1102

Belge Tipi:

Makale

Yayın Tarihi:

10/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001302511

pISSN:

2149-3367

Bacillus

Lipid

Staining

Sudan Black BBacillus

Lipid

Boyama

,Sudan Siyahı B

Sayı

Makale detayları

Yazarlar: Elif Demirkan | İrem Yıldırım

Yayıncı: Afyon Kocatepe University

Ana Başlıklar: Endüstriyel Biyoteknoloji

Dergi: Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi Vol.23 Issue 5, pp.1094-1102

Belge Tipi: Makale

Yayın Tarihi: 10/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001302511

pISSN: 2149-3367

Sayı

Yazarlar:

Elif Demirkan | İrem Yıldırım

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Endüstriyel Biyoteknoloji

Dergi:

Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi Vol.23 Issue 5, pp.1094-1102

Belge Tipi:

Makale

Yayın Tarihi:

10/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001302511

pISSN:

2149-3367