Vulpinic Acid Targets WNT/β-Catenin Signalling Pathway in HeLa Cells

Şeyda Nur Kalin

Makale detayları

Vulpinic Acid Targets WNT/β-Catenin Signalling Pathway in HeLa Cells

Yazarlar:

Şeyda Nur Kalin

Yayıncı:

Erzincan Binali Yildirim University

Ana Başlıklar:

Tıbbi Farmakoloji

Dergi:

Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.18 Issue 2, pp.495-504

Belge Tipi:

Makale

Yayın Tarihi:

8/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001356180

pISSN:

2149-4584

Anahtar kelimeler:

Özet:

 Cervical cancer is one of the most common cancers in women. Due to the side effects and inadequate treatment methods of current cancer drugs used in the treatment of cervical cancer, it is important to develop new treatment strategies. Vulpinic acid VA, a natural lichen secondary metabolite with many remarkable biological activities, has no detailed study describing its potential anti-cancer molecular mechanism in cervical cancer HeLa cell line. We reported in our previous study that VA exhibited anti-proliferative, apoptotic, and anti-migratory properties in HeLa cells and the IC50 dose of VA in HeLa cells was calculated as 66.53 µgmL at 48 h. The effect of VA on the WNTβ-catenin signaling pathway, which plays a role in various biological processes including tumorigenesis, cell proliferation, cell cycle regulation, embryogenesis, metastasis, cellular differentiation, apoptosis and drug resistance, is unknown. In this study, we aimed to elucidate whether VA exerts its antimigratory effect on HeLa cells treated with IC50 dose through the WNTβ-catenin signalling pathway. In summary, this study demonstrated that the suppression of migration of HeLa cells by VA may be mediated by inhibition of the WNTβ-catenin signalling pathway. VA may be a natural active compound candidate for the therapy of human cervical cancer and may be among the inhibitory candidates of the WNTβ-catenin signalling pathway.

 Cervical cancer is one of the most common cancers in women. Due to the side effects and inadequate treatment methods of current cancer drugs used in the treatment of cervical cancer, it is important to develop new treatment strategies. Vulpinic acid VA, a natural lichen secondary metabolite with many remarkable biological activities, has no detailed study describing its potential anti-cancer molecular mechanism in cervical cancer HeLa cell line. We reported in our previous study that VA exhibited anti-proliferative, apoptotic, and anti-migratory properties in HeLa cells and the IC50 dose of VA in HeLa cells was calculated as 66.53 µgmL at 48 h. The effect of VA on the WNTβ-catenin signaling pathway, which plays a role in various biological processes including tumorigenesis, cell proliferation, cell cycle regulation, embryogenesis, metastasis, cellular differentiation, apoptosis and drug resistance, is unknown. In this study, we aimed to elucidate whether VA exerts its antimigratory effect on HeLa cells treated with IC50 dose through the WNTβ-catenin signalling pathway. In summary, this study demonstrated that the suppression of migration of HeLa cells by VA may be mediated by inhibition of the WNTβ-catenin signalling pathway. VA may be a natural active compound candidate for the therapy of human cervical cancer and may be among the inhibitory candidates of the WNTβ-catenin signalling pathway.

 Cervical cancer is one of the most common cancers in women. Due to the side effects and inadequate treatment methods of current cancer drugs used in the treatment of cervical cancer, it is important to develop new treatment strategies. Vulpinic acid VA, a natural lichen secondary metabolite with many remarkable biological activities, has no detailed study describing its potential anti-cancer molecular mechanism in cervical cancer HeLa cell line. We reported in our previous study that VA exhibited anti-proliferative, apoptotic, and anti-migratory properties in HeLa cells and the IC50 dose of VA in HeLa cells was calculated as 66.53 µgmL at 48 h. The effect of VA on the WNTβ-catenin signaling pathway, which plays a role in various biological processes including tumorigenesis, cell proliferation, cell cycle regulation, embryogenesis, metastasis, cellular differentiation, apoptosis and drug resistance, is unknown. In this study, we aimed to elucidate whether VA exerts its antimigratory effect on HeLa cells treated with IC50 dose through the WNTβ-catenin signalling pathway. In summary, this study demonstrated that the suppression of migration of HeLa cells by VA may be mediated by inhibition of the WNTβ-catenin signalling pathway. VA may be a natural active compound candidate for the therapy of human cervical cancer and may be among the inhibitory candidates of the WNTβ-catenin signalling pathway.

 Rahim ağzı kanseri kadınlarda en sık görülen kanserlerden biridir. Rahim ağzı kanseri tedavisinde kullanılan mevcut kanser ilaçlarının yan etkileri ve yetersiz tedavi yöntemleri nedeniyle yeni tedavi stratejilerinin geliştirilmesi önem arz etmektedir. Birçok dikkate değer biyolojik aktiviteye sahip doğal bir liken sekonder metaboliti olan vulpinik asit VA, serviks kanseri HeLa hücre hattında potansiyel anti-kanser moleküler mekanizmasını açıklayan ayrıntılı bir çalışmaya sahip değildir. Önceki çalışmamızda VAnın HeLa hücrelerinde anti-proliferatif, apoptotik ve anti-göç özellikleri sergilediğini ve VAnın HeLa hücrelerindeki IC50 dozunun 48 saatte 66,53 µgmL olarak hesaplandığını bildirmiştik. VAnın tümörigenez, hücre proliferasyonu, hücre döngüsü düzenlemesi, embriyogenez, metastaz, hücresel farklılaşma, apoptoz ve ilaç direnci gibi çeşitli biyolojik süreçlerde rol oynayan WNTβ-katenin sinyal yolağı üzerindeki etkisi bilinmemektedir. Bu çalışmada, VAnın IC50 dozu ile tedavi edilen HeLa hücreleri üzerindeki antimigratör etkisini WNTβ-katenin sinyal yolağı üzerinden gösterip göstermediğini aydınlatmayı amaçladık. Özetle, bu çalışma HeLa hücrelerinin göçünün VA tarafından baskılanmasına WNTβ-katenin sinyal yolağının inhibisyonunun aracılık edebileceğini göstermiştir. VA, insan serviks kanserinin tedavisi için doğal bir aktif bileşik adayı olabilir ve WNTβ-katenin sinyal yolağının inhibitör adayları arasında yer alabilir.

Makale detayları

Vulpinic Acid Targets WNT/β-Catenin Signalling Pathway in HeLa Cells

Yazarlar:

Şeyda Nur Kalin

Yayıncı:

Erzincan Binali Yildirim University

Ana Başlıklar:

Tıbbi Farmakoloji

Dergi:

Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.18 Issue 2, pp.495-504

Belge Tipi:

Makale

Yayın Tarihi:

8/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001356180

pISSN:

2149-4584

lichen secondary metabolite

antimigratory effect

gene expressionliken sekonder metabolit

anti-migratif etki

gen ekspresyonu

Sayı

Makale detayları

Vulpinic Acid Targets WNT/β-Catenin Signalling Pathway in HeLa Cells

Yazarlar: Şeyda Nur Kalin

Yayıncı: Erzincan Binali Yildirim University

Ana Başlıklar: Tıbbi Farmakoloji

Dergi: Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.18 Issue 2, pp.495-504

Belge Tipi: Makale

Yayın Tarihi: 8/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001356180

pISSN: 2149-4584

lichen secondary metabolite

antimigratory effect

gene expressionliken sekonder metabolit

anti-migratif etki

gen ekspresyonu

Sayı

Yazarlar:

Şeyda Nur Kalin

Yayıncı:

Erzincan Binali Yildirim University

Ana Başlıklar:

Tıbbi Farmakoloji

Dergi:

Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.18 Issue 2, pp.495-504

Belge Tipi:

Makale

Yayın Tarihi:

8/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001356180

pISSN:

2149-4584