Genetic characterization of Varroa destructor (Family: Varroidae) prevalent in honeybees (Apis mellifera) in the province of Aydin in Turkey

Adnan AYAN; Osman Selçuk ALDEMİR

Makale detayları

Genetic characterization of Varroa destructor (Family: Varroidae) prevalent in honeybees (Apis mellifera) in the province of Aydin in Turkey

Yazarlar:

Adnan AYAN | Osman Selçuk ALDEMİR

Yayıncı:

Burdur Mehmet Akif Ersoy University

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

Mehmet Akif Ersoy University Journal of Health Sciences Institute 2018 Vol.6 Issue 1, pp.26-32

Belge Tipi:

Makale

Yayın Tarihi:

7/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000552165

Sıklık:

Üç Yıllık

pISSN:

2148-2837

Özet:

 


The aim of the present study was to identify
the haplotypes of the Varroa destructor mite which infects honeybees in
the province of Aydın in Turkey, using two different modified techniques for
the mitochondrial Cox1 gene of the mite.
In order to confirm the haplotype, two primers differing in their sequence i.e.
(ADA 01) as
forward primer 5′-TACAAAGAGGGAAGAAGCAGCC-3′ and reverse primer 5′-
GCCCCTATTCTTAATACATAGTGAAAATG-3′ and (ADA 02) with COXF primer
[5′GG(A/G)GG(A/T)GA(C/T)CC(A/T)ATT(C/T)T(A/T)TATCAAC3′] and COXRa primer
[5′GG(A/T)GACCTGT(A/TA(A/T)AATAGCAAATAC3′], were selected.
Amplified DNA 376 bp in size was acquired using (ADA 01) forward primer 5′-
TACAAAGAGGGAAGAAGCAGCC-3′ and reverse primer 5′-
GCCCCTATTCTTAATACATAGTGAAAATG-3′. SacI restriction enzyme was applied to
the amplified products; however, this restriction enzyme did not cut the DNA.
Amplified DNA, 570 bp in size was obtained using (ADA 02) COXF primer
[5’GG(A/G)GG(A/T)GA(C/T)CC(A/T)ATT(C/T)T(A/T)TATCAAC3’] COXRa and
[5’GG(A/T)GACCTGT(A/TA(A/T)AATAGCAAATAC3’]. XhoI and SacI
restriction
enzymes were used for the amplified products. Although, the SacI
restriction enzyme did not cut the DNA, the XhoI restriction enzyme cut
the amplified DNA into two fragments (bands), with the sizes of 270 and 300 bp
two bands 270 and 300 bp. While comparing the results, these bands were found
specific for Korean haplotype of V. destructor.
In conclusion, all of the 200 samples of V. destructor examined in this
study were identified to be the Korean haplotype.


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Makale detayları

Genetic characterization of Varroa destructor (Family: Varroidae) prevalent in honeybees (Apis mellifera) in the province of Aydin in Turkey

Yazarlar: Adnan AYAN | Osman Selçuk ALDEMİR

Yayıncı: Burdur Mehmet Akif Ersoy University

Ana Başlıklar: Sağlık teşvikleri

Dergi: Mehmet Akif Ersoy University Journal of Health Sciences Institute 2018 Vol.6 Issue 1, pp.26-32

Belge Tipi: Makale

Yayın Tarihi: 7/2018

Belge Dili: İngilizce

Document ID (Org No.): 2000552165

Sıklık: Üç Yıllık

pISSN: 2148-2837

Sayı

Yazarlar:

Adnan AYAN | Osman Selçuk ALDEMİR

Yayıncı:

Burdur Mehmet Akif Ersoy University

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

Mehmet Akif Ersoy University Journal of Health Sciences Institute 2018 Vol.6 Issue 1, pp.26-32

Belge Tipi:

Makale

Yayın Tarihi:

7/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000552165

Sıklık:

Üç Yıllık

pISSN:

2148-2837