INVESTIGATION OF BIOCHEMICAL PROPERTIES, ANTIMICROBIAL, ANTIOXIDANT AND ANTIPROLIFERATIVE ACTIVITY OF THE RHAMNUS CATHARTICA L. EXTRACTS COLLECTED FROM KAZAKHSTAN

Sofiakhan Alimova; Yessimsiitova Zura; Tutku Tunç; Hülya Özpınar

Makale detayları

INVESTIGATION OF BIOCHEMICAL PROPERTIES, ANTIMICROBIAL, ANTIOXIDANT AND ANTIPROLIFERATIVE ACTIVITY OF THE RHAMNUS CATHARTICA L. EXTRACTS COLLECTED FROM KAZAKHSTAN

Yazarlar:

Sofiakhan Alimova | Yessimsiitova Zura | Tutku Tunç | Hülya Özpınar

Yayıncı:

Ankara University

Ana Başlıklar:

Farmasotik Botanik | Farmasotik Mikrobiyoloji

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.49 Issue 3, pp.743-755

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001356404

pISSN:

1015-3918

Anahtar kelimeler:

Özet:

 Objectives: This research evaluated the biochemical composition of hexane, ethyl acetate, and ethanol extracts and analyzed the antimicrobial, antioxidant and antiproliferative activities of the cortex, leaf, and fruit of the Rhamnus cathartica L. plant from Almaty, Kazakhstan. Material and Method: The GC-MS method was used to evaluate the chemicals of Rhamnus cathartica L. cortex, leaf, and fruit extracts. The microdilution technique was used to evaluate the MIC values of plant extracts against pathogenic microorganisms. DPPH, FRAP, and Total Flavonoid Content were evaluated for antioxidant activity. The antiproliferative effect of the extracts on MCF-7, A549, C6 and WI-38 cells was measured by XTT assay. Result and Discussion: According to GC-MS results, Trans-2-Decenal had the highest proportion at 2.88. Eicosane is second at 1.71. DPPH, FRAP and flavonoid values showed that the fruit ethyl acetate extract F-EtAc had the strongest antioxidant activity. F-EtAc extract had the strongest anticancer activity according to the selectivity index table WI-38MCF-7: 12.67, WI-38C6: 31.38, WI-38A549: 8.55. All plant extracts had high MIC values against Staphylococcus aureus based on reference source values. The MIC value of cortex and leaf in ethyl acetate was 0.078 mgml, cortex and leaf extracts had strong antimicrobial activity 0.312 mgml for Bacillus cereus, while fruits had weakly effective MIC values SignificantMIC 0.1 mgml. According to the results of this study, it can be said that Rhamnus cathartica L. plant extracts have an active content in terms of biological properties.

 Objectives: This research evaluated the biochemical composition of hexane, ethyl acetate, and ethanol extracts and analyzed the antimicrobial, antioxidant and antiproliferative activities of the cortex, leaf, and fruit of the Rhamnus cathartica L. plant from Almaty, Kazakhstan. Material and Method: The GC-MS method was used to evaluate the chemicals of Rhamnus cathartica L. cortex, leaf, and fruit extracts. The microdilution technique was used to evaluate the MIC values of plant extracts against pathogenic microorganisms. DPPH, FRAP, and Total Flavonoid Content were evaluated for antioxidant activity. The antiproliferative effect of the extracts on MCF-7, A549, C6 and WI-38 cells was measured by XTT assay. Result and Discussion: According to GC-MS results, Trans-2-Decenal had the highest proportion at 2.88. Eicosane is second at 1.71. DPPH, FRAP and flavonoid values showed that the fruit ethyl acetate extract F-EtAc had the strongest antioxidant activity. F-EtAc extract had the strongest anticancer activity according to the selectivity index table WI-38MCF-7: 12.67, WI-38C6: 31.38, WI-38A549: 8.55. All plant extracts had high MIC values against Staphylococcus aureus based on reference source values. The MIC value of cortex and leaf in ethyl acetate was 0.078 mgml, cortex and leaf extracts had strong antimicrobial activity 0.312 mgml for Bacillus cereus, while fruits had weakly effective MIC values SignificantMIC 0.1 mgml. According to the results of this study, it can be said that Rhamnus cathartica L. plant extracts have an active content in terms of biological properties.

 Objectives: This research evaluated the biochemical composition of hexane, ethyl acetate, and ethanol extracts and analyzed the antimicrobial, antioxidant and antiproliferative activities of the cortex, leaf, and fruit of the Rhamnus cathartica L. plant from Almaty, Kazakhstan. Material and Method: The GC-MS method was used to evaluate the chemicals of Rhamnus cathartica L. cortex, leaf, and fruit extracts. The microdilution technique was used to evaluate the MIC values of plant extracts against pathogenic microorganisms. DPPH, FRAP, and Total Flavonoid Content were evaluated for antioxidant activity. The antiproliferative effect of the extracts on MCF-7, A549, C6 and WI-38 cells was measured by XTT assay. Result and Discussion: According to GC-MS results, Trans-2-Decenal had the highest proportion at 2.88. Eicosane is second at 1.71. DPPH, FRAP and flavonoid values showed that the fruit ethyl acetate extract F-EtAc had the strongest antioxidant activity. F-EtAc extract had the strongest anticancer activity according to the selectivity index table WI-38MCF-7: 12.67, WI-38C6: 31.38, WI-38A549: 8.55. All plant extracts had high MIC values against Staphylococcus aureus based on reference source values. The MIC value of cortex and leaf in ethyl acetate was 0.078 mgml, cortex and leaf extracts had strong antimicrobial activity 0.312 mgml for Bacillus cereus, while fruits had weakly effective MIC values SignificantMIC 0.1 mgml. According to the results of this study, it can be said that Rhamnus cathartica L. plant extracts have an active content in terms of biological properties.

 Amaç: Bu araştırmada, Kazakistanın Almatı kentinden elde edilen Rhamnus cathartica L. bitkisinin korteks, yaprak ve meyvesinin hekzan, etil asetat ve etanol özütlerinin biyokimyasal bileşimi değerlendirilmiş ve antimikrobiyal, antioksidan ve antiproliferatif aktiviteleri analiz edilmiştir. Gereç ve Yöntem: Rhamnus cathartica L. korteks, yaprak ve meyve özütü kimyasallarını belirlemek için GC-MS yöntemi kullanıldı. Bitki ekstraktlarının patojen mikroorganizmalara karşı MİK değerlerini değerlendirmek için mikrodilüsyon yöntemi kullanılmıştır. Antioksidan aktivite tayini için DPPH, FRAP ve Toplam flavonoid içeriği belirlenmiştir. Ekstraktların MCF-7, A549, C6 ve WI-38 hücreleri üzerine antiproliferatif etkisi XTT testi ile ölçülmüştür. Sonuç ve Tartışma: GC-MS sonuçlarına göre; Trans-2-Decenal 2.88 ile en yüksek orana sahiptir. Eicosane 1.71 ile ikinci sıradadır. DPPH, FRAP ve flavonoid değerleri meyve etil asetat ekstresinin F-EtAc en güçlü antioksidan aktiviteye sahip olduğunu göstermiştir. F-EtAc ekstresi, seçicilik indeksi tablosuna göre en güçlü antikanser aktivitesine sahiptir WI-38MCF-7: 12.67, WI-38C6: 31.38, WI-38A549: 8.55. Tüm bitki ekstraktları, referans kaynak değerlerine göre Staphylococcus aureusa karşı anlamlı MİK değerlerine ulaşmıştır. Korteks ve yaprağın etil asetat içindeki MİK değeri 0.078 mgml, korteks ve yaprak ekstraktları Bacillus cereus için anlamlı antimikrobiyal aktiviteye 0.312 mgml sahipken, meyveler zayıf etkili MİK değerlerine ulaşmıştır AnlamlıMİK 0.1 mgml. Bu çalışmanın sonuçlarna göre, Rhamnus cathartica L. bitki ekstraktlarının biyolojik özellikler açısından aktif bir içeriğe sahip olduğu söylenebilir.

Makale detayları

INVESTIGATION OF BIOCHEMICAL PROPERTIES, ANTIMICROBIAL, ANTIOXIDANT AND ANTIPROLIFERATIVE ACTIVITY OF THE RHAMNUS CATHARTICA L. EXTRACTS COLLECTED FROM KAZAKHSTAN

Yazarlar:

Sofiakhan Alimova | Yessimsiitova Zura | Tutku Tunç | Hülya Özpınar

Yayıncı:

Ankara University

Ana Başlıklar:

Farmasotik Botanik | Farmasotik Mikrobiyoloji

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.49 Issue 3, pp.743-755

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001356404

pISSN:

1015-3918

Antimicrobial

antioxidant

antiproliferative

Rhamnus catharticaAntimikrobiyal

antioksidan

antiproliferatif

Rhamnus cathartica

Sayı

Makale detayları

Yazarlar: Sofiakhan Alimova | Yessimsiitova Zura | Tutku Tunç | Hülya Özpınar

Yayıncı: Ankara University

Ana Başlıklar: Farmasotik Botanik | Farmasotik Mikrobiyoloji

Dergi: Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.49 Issue 3, pp.743-755

Belge Tipi: Makale

Yayın Tarihi: 9/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001356404

pISSN: 1015-3918

Sayı

Yazarlar:

Sofiakhan Alimova | Yessimsiitova Zura | Tutku Tunç | Hülya Özpınar

Yayıncı:

Ankara University

Ana Başlıklar:

Farmasotik Botanik | Farmasotik Mikrobiyoloji

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.49 Issue 3, pp.743-755

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001356404

pISSN:

1015-3918