The development of forced degradation studies for the determination of daptomycin in urine at various conditions by using RP-LC method

Nurgul Karadas Bakirhan; Tuğçe Göver

Makale detayları

The development of forced degradation studies for the determination of daptomycin in urine at various conditions by using RP-LC method

Yazarlar:

Nurgul Karadas Bakirhan | Tuğçe Göver

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Analytical Chemistry | Pharmaceutical Chemistry

Dergi:

Journal of Research in Pharmacy Vol.23 Issue 2, pp.170-176

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346471

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 Daptomycin is known as the first lipopeptide antibiotic shows activity against gram-positive bacteria. A fast, simple and repeatabile RP-LC method has been developed for the determination of daptomycin in mobile phase solution and human urine. This drug was exposed to various stress conditions such as hydrolytic, oxidative, thermal and photolytic and the stressed samples were detected by the proposed method. Daptomycin was well separated on a X-Terra RP-18 column using the mobile phase consisting of a mixture of acetonitrile:water 45:55 vv at a flow rate of 1 mL min-1. The system operates at 222 nm for daptomycin and 319 nm and tinidazole was used as internal standard under these conditions. The linearity of daptomycin was obtained between 0.50 and 12 µg mL-1 concentration range. Limits of detection was determined as 0.1176 and 0.1412 µg mL-1 and the limits of quantification was obtained as 0.3563 and 0.4277 µg mL-1 in the mobile phase and urine, respectively. Hydrochloric acid, sodium hydroxide, hydrogen peroxide affected response of daptomycin under stress degradation conditions.

 Daptomycin is known as the first lipopeptide antibiotic shows activity against gram-positive bacteria. A fast, simple and repeatabile RP-LC method has been developed for the determination of daptomycin in mobile phase solution and human urine. This drug was exposed to various stress conditions such as hydrolytic, oxidative, thermal and photolytic and the stressed samples were detected by the proposed method. Daptomycin was well separated on a X-Terra RP-18 column using the mobile phase consisting of a mixture of acetonitrile:water 45:55 vv at a flow rate of 1 mL min-1. The system operates at 222 nm for daptomycin and 319 nm and tinidazole was used as internal standard under these conditions. The linearity of daptomycin was obtained between 0.50 and 12 µg mL-1 concentration range. Limits of detection was determined as 0.1176 and 0.1412 µg mL-1 and the limits of quantification was obtained as 0.3563 and 0.4277 µg mL-1 in the mobile phase and urine, respectively. Hydrochloric acid, sodium hydroxide, hydrogen peroxide affected response of daptomycin under stress degradation conditions.

 Daptomycin is known as the first lipopeptide antibiotic shows activity against gram-positive bacteria. A fast, simple and repeatabile RP-LC method has been developed for the determination of daptomycin in mobile phase solution and human urine. This drug was exposed to various stress conditions such as hydrolytic, oxidative, thermal and photolytic and the stressed samples were detected by the proposed method. Daptomycin was well separated on a X-Terra RP-18 column using the mobile phase consisting of a mixture of acetonitrile:water 45:55 vv at a flow rate of 1 mL min-1. The system operates at 222 nm for daptomycin and 319 nm and tinidazole was used as internal standard under these conditions. The linearity of daptomycin was obtained between 0.50 and 12 µg mL-1 concentration range. Limits of detection was determined as 0.1176 and 0.1412 µg mL-1 and the limits of quantification was obtained as 0.3563 and 0.4277 µg mL-1 in the mobile phase and urine, respectively. Hydrochloric acid, sodium hydroxide, hydrogen peroxide affected response of daptomycin under stress degradation conditions.

 Daptomycin is known as the first lipopeptide antibiotic shows activity against gram-positive bacteria. A fast, simple and repeatabile RP-LC method has been developed for the determination of daptomycin in mobile phase solution and human urine. This drug was exposed to various stress conditions such as hydrolytic, oxidative, thermal and photolytic and the stressed samples were detected by the proposed method. Daptomycin was well separated on a X-Terra RP-18 column using the mobile phase consisting of a mixture of acetonitrile:water 45:55 vv at a flow rate of 1 mL min-1. The system operates at 222 nm for daptomycin and 319 nm and tinidazole was used as internal standard under these conditions. The linearity of daptomycin was obtained between 0.50 and 12 µg mL-1 concentration range. Limits of detection was determined as 0.1176 and 0.1412 µg mL-1 and the limits of quantification was obtained as 0.3563 and 0.4277 µg mL-1 in the mobile phase and urine, respectively. Hydrochloric acid, sodium hydroxide, hydrogen peroxide affected response of daptomycin under stress degradation conditions.

Makale detayları

The development of forced degradation studies for the determination of daptomycin in urine at various conditions by using RP-LC method

Yazarlar:

Nurgul Karadas Bakirhan | Tuğçe Göver

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Analytical Chemistry | Pharmaceutical Chemistry

Dergi:

Journal of Research in Pharmacy Vol.23 Issue 2, pp.170-176

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346471

pISSN:

2630-6344

Drug

daptomycin

degradation conditions

high performance liquid chromatography

Sayı

Makale detayları

The development of forced degradation studies for the determination of daptomycin in urine at various conditions by using RP-LC method

Yazarlar: Nurgul Karadas Bakirhan | Tuğçe Göver

Yayıncı: Marmara University

Ana Başlıklar: Pharmaceutical Analytical Chemistry | Pharmaceutical Chemistry

Dergi: Journal of Research in Pharmacy Vol.23 Issue 2, pp.170-176

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001346471

pISSN: 2630-6344

Drug

daptomycin

degradation conditions

high performance liquid chromatography

Sayı

Yazarlar:

Nurgul Karadas Bakirhan | Tuğçe Göver

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Analytical Chemistry | Pharmaceutical Chemistry

Dergi:

Journal of Research in Pharmacy Vol.23 Issue 2, pp.170-176

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346471

pISSN:

2630-6344