Evaluation of the genetic relationship between Fritillaria species from Turkey&#8217;s flora using fluorescent-based AFLP

Özge Karakaş METİN; Mine TÜRKTAŞ; Meral ASLAY; Erdal KAYA

Makale detayları

Evaluation of the genetic relationship between Fritillaria species from Turkey’s flora using fluorescent-based AFLP

Yazarlar:

Özge Karakaş METİN | Mine TÜRKTAŞ | Meral ASLAY | Erdal KAYA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2013 Vol.37 Issue 3, pp.273-279

Belge Tipi:

Makale

Yayın Tarihi:

2/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000812428

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

In this study, the genetic relationships among 12 Fritillaria taxa collected from different regions of Turkey were investigated using the amplified fragment length polymorphism (AFLP) technique. Seven primer pair combinations were used for the analysis. The neighbor-joining and principal coordinate analysis methods were implemented to identify the taxonomy of the species. The analyses generated congruent results with each other and with the previous studies. The neighbor-joining analysis clustered the species into 3 groups, while the principal coordinates analysis did not support separation of the third group. The subgenera Fritillaria and Petilium diverged from each other with strong support in all analyses. The neighbor-joining tree further divided the members of subgenus Fritillaria into 2 subgroups. Statistical analysis of the data set using NTSYS 2.1 showed a very good fit between the data matrix and the cophenetic matrix for the AFLP data with an r-value of 0.91. Generating a high polymorphic band profile, this analysis presented the feasibility of using AFLP for a genetic relationship analysis of Fritillaria. This primary report on AFLP analysis of Fritillaria species could facilitate achieving a deeper understanding of genetic relationships between the species.

 

In this study, the genetic relationships among 12 Fritillaria taxa collected from different regions of Turkey were investigated using the amplified fragment length polymorphism (AFLP) technique. Seven primer pair combinations were used for the analysis. The neighbor-joining and principal coordinate analysis methods were implemented to identify the taxonomy of the species. The analyses generated congruent results with each other and with the previous studies. The neighbor-joining analysis clustered the species into 3 groups, while the principal coordinates analysis did not support separation of the third group. The subgenera Fritillaria and Petilium diverged from each other with strong support in all analyses. The neighbor-joining tree further divided the members of subgenus Fritillaria into 2 subgroups. Statistical analysis of the data set using NTSYS 2.1 showed a very good fit between the data matrix and the cophenetic matrix for the AFLP data with an r-value of 0.91. Generating a high polymorphic band profile, this analysis presented the feasibility of using AFLP for a genetic relationship analysis of Fritillaria. This primary report on AFLP analysis of Fritillaria species could facilitate achieving a deeper understanding of genetic relationships between the species.

Makale detayları

Evaluation of the genetic relationship between Fritillaria species from Turkey’s flora using fluorescent-based AFLP

Yazarlar:

Özge Karakaş METİN | Mine TÜRKTAŞ | Meral ASLAY | Erdal KAYA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2013 Vol.37 Issue 3, pp.273-279

Belge Tipi:

Makale

Yayın Tarihi:

2/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000812428

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Key words: AFLP

Fritillaria

neighbor joining

PCoA

genetic relationships

Sayı

Makale detayları

Evaluation of the genetic relationship between Fritillaria species from Turkey&#8217;s flora using fluorescent-based AFLP

Yazarlar: Özge Karakaş METİN | Mine TÜRKTAŞ | Meral ASLAY | Erdal KAYA

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2013 Vol.37 Issue 3, pp.273-279

Belge Tipi: Makale

Yayın Tarihi: 2/2013

Belge Dili: Türkçe

Document ID (Org No.): 2000812428

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Key words: AFLP

Fritillaria

neighbor joining

PCoA

genetic relationships

Sayı

Evaluation of the genetic relationship between Fritillaria species from Turkey’s flora using fluorescent-based AFLP

Yazarlar:

Özge Karakaş METİN | Mine TÜRKTAŞ | Meral ASLAY | Erdal KAYA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2013 Vol.37 Issue 3, pp.273-279

Belge Tipi:

Makale

Yayın Tarihi:

2/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000812428

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152