Characterization of Catalase Enzyme from Leaf Tissue of Aronia (Aronia melanocarpa) Plant

Ömer Taş; Betül Mitrovica; Deniz Ekinci

Makale detayları

Characterization of Catalase Enzyme from Leaf Tissue of Aronia (Aronia melanocarpa) Plant

Yazarlar:

Ömer Taş | Betül Mitrovica | Deniz Ekinci

Yayıncı:

Ondokuz Mayıs University

Dergi:

Anadolu Tarım Bilimleri Dergisi Vol.38 Issue 1, pp.199-208

Belge Tipi:

Makale

Yayın Tarihi:

2/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001283743

pISSN:

1308-8750

Anahtar kelimeler:

Özet:

 Aronia is among the most antioxidant containing plants which is found commonly around the world. Aronia cultivation started in Turkey for the first time in 2012 at the Atatürk Central Research Institute of Garden Cultures, and a plantation was constructed in the experimental area. Since antioxidants help to preserve food by blocking oxidation processes and contributing to the health promotion provided by numerous dietary supplements, nutraceutical and functional food additives, antioxidant capacity of these plants should be well characterized. To assess and evaluate the antioxidant content of foods and plant products, many approaches are utilized. In this study, catalase CAT enzyme was partially purified from aronia plant leaf tissue and characterization was carried out. Purification process consisted of homogenate preparation, ammonium sulfate precipitation and dialysis. The optimal ionic strength, pH, substrate concentration and enzyme quantity were examined. These values were found to be 300 mM Tris, pH:8.0, 12 mM H 2 O 2 and 75 μl, respectively, for the catalase enzyme of the Aronia plant leaf tissue. This study is the first in the literature dealing with the characterization of antioxidant enzyme from Aronia plant.

 Aronia is among the most antioxidant containing plants which is found commonly around the world. Aronia cultivation started in Turkey for the first time in 2012 at the Atatürk Central Research Institute of Garden Cultures, and a plantation was constructed in the experimental area. Since antioxidants help to preserve food by blocking oxidation processes and contributing to the health promotion provided by numerous dietary supplements, nutraceutical and functional food additives, antioxidant capacity of these plants should be well characterized. To assess and evaluate the antioxidant content of foods and plant products, many approaches are utilized. In this study, catalase CAT enzyme was partially purified from aronia plant leaf tissue and characterization was carried out. Purification process consisted of homogenate preparation, ammonium sulfate precipitation and dialysis. The optimal ionic strength, pH, substrate concentration and enzyme quantity were examined. These values were found to be 300 mM Tris, pH:8.0, 12 mM H 2 O 2 and 75 μl, respectively, for the catalase enzyme of the Aronia plant leaf tissue. This study is the first in the literature dealing with the characterization of antioxidant enzyme from Aronia plant.

 Aronia is among the most antioxidant containing plants which is found commonly around the world. Aronia cultivation started in Turkey for the first time in 2012 at the Atatürk Central Research Institute of Garden Cultures, and a plantation was constructed in the experimental area. Since antioxidants help to preserve food by blocking oxidation processes and contributing to the health promotion provided by numerous dietary supplements, nutraceutical and functional food additives, antioxidant capacity of these plants should be well characterized. To assess and evaluate the antioxidant content of foods and plant products, many approaches are utilized. In this study, catalase CAT enzyme was partially purified from aronia plant leaf tissue and characterization was carried out. Purification process consisted of homogenate preparation, ammonium sulfate precipitation and dialysis. The optimal ionic strength, pH, substrate concentration and enzyme quantity were examined. These values were found to be 300 mM Tris, pH:8.0, 12 mM H 2 O 2 and 75 μl, respectively, for the catalase enzyme of the Aronia plant leaf tissue. This study is the first in the literature dealing with the characterization of antioxidant enzyme from Aronia plant.

 Aronia, dünya çapında yaygın olarak bulunan en çok antioksidan içeren bitkilerden biridir. Türkiyede ilk kez 2012 yılında Atatürk Bahçe Kültürleri Merkez Araştırma Enstitüsünde Aronia yetiştiriciliğine başlanmış ve deneme alanına ağaçlandırma yapılmıştır. Antioksidanlar, oksidasyon süreçlerini bloke ederek ve çok sayıda diyet takviyesi, nutrasötik ve fonksiyonel gıda katkı maddesi tarafından sağlanan sağlığın önemine katkıda bulunarak gıdaların korunmasına yardımcı olduklarından, bu bitkilerin antioksidan kapasiteleri iyi karakterize edilmelidir. Gıdaların ve bitkisel ürünlerin antioksidan içeriğini değerlendirmek ve belirlemek için birçok yaklaşım kullanılmaktadır. Bu çalışmada aronia bitkisinin yaprak dokusundan katalaz CAT enzimi kısmen saflaştırılmış ve karakterizasyonu yapılmıştır. Saflaştırma işlemi, homojenat hazırlama, amonyum sülfat çökeltme ve diyalizden oluşmaktadır. Optimum iyonik kuvvet, pH, substrat konsantrasyonu ve enzim miktarı incelenmiştir. Bu değerler Aronia bitki yaprak dokusunun katalaz enzimi için sırasıyla 300 mM Tris, pH:8.0, 12 mM H2O2 ve 75 µl olarak bulunmuştur. Bu çalışma Aronia bitkisinden elde edilen antioksidan enzimin karakterizasyonu ile ilgili literatürdeki ilk çalışmadır.

Makale detayları

Characterization of Catalase Enzyme from Leaf Tissue of Aronia (Aronia melanocarpa) Plant

Yazarlar:

Ömer Taş | Betül Mitrovica | Deniz Ekinci

Yayıncı:

Ondokuz Mayıs University

Dergi:

Anadolu Tarım Bilimleri Dergisi Vol.38 Issue 1, pp.199-208

Belge Tipi:

Makale

Yayın Tarihi:

2/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001283743

pISSN:

1308-8750

Antioxidant

Aronia

Catalase

Characterization

Antioksidan

Aronya

Katalaz

Karakterizasyon

Antioxidant

Sayı

Makale detayları

Yazarlar: Ömer Taş | Betül Mitrovica | Deniz Ekinci

Yayıncı: Ondokuz Mayıs University

Dergi: Anadolu Tarım Bilimleri Dergisi Vol.38 Issue 1, pp.199-208

Belge Tipi: Makale

Yayın Tarihi: 2/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001283743

pISSN: 1308-8750

Sayı

Yazarlar:

Ömer Taş | Betül Mitrovica | Deniz Ekinci

Yayıncı:

Ondokuz Mayıs University

Dergi:

Anadolu Tarım Bilimleri Dergisi Vol.38 Issue 1, pp.199-208

Belge Tipi:

Makale

Yayın Tarihi:

2/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001283743

pISSN:

1308-8750