Investigation of Methylation and Expression Levels in STK32C Gene in Oral Malignant Lesions

Seval Turna; Comert Sen; Kağan Avcı; Murat Ulusan; Nejat Vakur Olgaç; Semra Demokan

Makale detayları

Investigation of Methylation and Expression Levels in STK32C Gene in Oral Malignant Lesions

Yazarlar:

Seval Turna | Comert Sen | Kağan Avcı | Murat Ulusan | Nejat Vakur Olgaç | Semra Demokan

Yayıncı:

Giresun University

Ana Başlıklar:

Öngörücü ve Prognostik Belirteçler

Dergi:

Doğu Karadeniz Sağlık Bilimleri Dergisi Vol.4 Issue 3, pp.265-271

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001349985

pISSN:

2822-6445

Anahtar kelimeler:

Özet:

 Aim: As a result of the array analyses obtained within the scope of the TUBITAK-SBAG-114S497 project conducted by Demokan and his colleagues, STK32C gene was determined as a potential epigenetic biomarker candidate. In this study, the methylation and expression levels of STK32C gene were examined in a larger patient group consisting of oral squamous cell carcinoma OSCC cases; the biomarker potential of this gene in terms of early diagnosisprognosis evaluation was investigated. Method: DNA and RNA were isolated from tissue samples of 15 OSCC patients, and the methylation and expression levels of the STK32C gene were examined using Quantitative Methylation Specific PCR and Real-Time PCR methods, respectively. Results and Conclusions: In tumor samples of OSCC patients, 93.3 methylation was observed in the promoter region of the STK32C gene. When expression levels were evaluated, expression loss was detected in 53.3 of the samples in tumor tissues compared to normal tissues, while expression increase was observed in 13.3. Hypermethylation was detected in all patients with decreased expression levels. It is suggested that the loss of expression observed in the STK32C gene due to hypermethylation may play a role in the molecular characterization of a certain subgroup of OSCC patients and that this gene may be a significant biomarker candidate for early diagnosis and prognosis. However, further studies covering larger patient populations are needed to confirm this.

 Aim: As a result of the array analyses obtained within the scope of the TUBITAK-SBAG-114S497 project conducted by Demokan and his colleagues, STK32C gene was determined as a potential epigenetic biomarker candidate. In this study, the methylation and expression levels of STK32C gene were examined in a larger patient group consisting of oral squamous cell carcinoma OSCC cases; the biomarker potential of this gene in terms of early diagnosisprognosis evaluation was investigated. Method: DNA and RNA were isolated from tissue samples of 15 OSCC patients, and the methylation and expression levels of the STK32C gene were examined using Quantitative Methylation Specific PCR and Real-Time PCR methods, respectively. Results and Conclusions: In tumor samples of OSCC patients, 93.3 methylation was observed in the promoter region of the STK32C gene. When expression levels were evaluated, expression loss was detected in 53.3 of the samples in tumor tissues compared to normal tissues, while expression increase was observed in 13.3. Hypermethylation was detected in all patients with decreased expression levels. It is suggested that the loss of expression observed in the STK32C gene due to hypermethylation may play a role in the molecular characterization of a certain subgroup of OSCC patients and that this gene may be a significant biomarker candidate for early diagnosis and prognosis. However, further studies covering larger patient populations are needed to confirm this.

 Aim: As a result of the array analyses obtained within the scope of the TUBITAK-SBAG-114S497 project conducted by Demokan and his colleagues, STK32C gene was determined as a potential epigenetic biomarker candidate. In this study, the methylation and expression levels of STK32C gene were examined in a larger patient group consisting of oral squamous cell carcinoma OSCC cases; the biomarker potential of this gene in terms of early diagnosisprognosis evaluation was investigated. Method: DNA and RNA were isolated from tissue samples of 15 OSCC patients, and the methylation and expression levels of the STK32C gene were examined using Quantitative Methylation Specific PCR and Real-Time PCR methods, respectively. Results and Conclusions: In tumor samples of OSCC patients, 93.3 methylation was observed in the promoter region of the STK32C gene. When expression levels were evaluated, expression loss was detected in 53.3 of the samples in tumor tissues compared to normal tissues, while expression increase was observed in 13.3. Hypermethylation was detected in all patients with decreased expression levels. It is suggested that the loss of expression observed in the STK32C gene due to hypermethylation may play a role in the molecular characterization of a certain subgroup of OSCC patients and that this gene may be a significant biomarker candidate for early diagnosis and prognosis. However, further studies covering larger patient populations are needed to confirm this.

 Amaç: Demokan ve arkadaşları tarafından yürütülen TÜBİTAK-SBAG-114S497 kodlu proje kapsamında elde edilen array analizleri sonucunda, STK32C geni potansiyel bir epigenetik biyobelirteç adayı olarak belirlenmiştir. Bu çalışmada, oral skuamöz hücreli karsinom OSCC olgularından oluşan geniş bir hasta grubunda STK32C gen metilasyonu ve ekspresyonu düzeyleri incelenmiş; bu genin erken tanıprognoz değerlendirme açısından biyobelirteç potansiyeli araştırılmıştır. Yöntem: 15 OSCC hastasının doku örneklerinden DNA ve RNA izole edilmiş ve STK32C gen metilasyonu ve ekspresyonu düzeyleri sırasıyla Kantitatif Metilasyon Spesifik PCR ve Gerçek Zamanlı PCR yöntemleri kullanılarak incelenmiştir. Bulgular ve Sonuçlar: OSCC hastalarının tümör örneklerinde, STK32C geni promotör bölgesinde 93,3 metilasyon gözlenmiştir. Ekspresyon seviyeleri değerlendirildiğinde, tümörlü dokulardaki örneklerin 53,3ünde normal dokulara kıyasla ekspresyon kaybı, 13,3ünde ise ekspresyon artışı tespit edilmiştir. Ekspresyon seviyeleri düşük olan tüm hastalarda hipermetilasyon saptanmıştır. Hipermetilasyona bağlı STK32C geninde gözlenen ekspresyon kaybının, OSCC hastalarının belirli bir alt grubunun moleküler karakterizasyonunda rol oynayabileceği ve bu genin erken tanı ve prognoz için önemli bir biyobelirteç adayı olabileceği düşünülmektedir. Ancak, bunu doğrulamak için daha geniş hasta popülasyonlarını kapsayan daha fazla çalışmaya ihtiyaç bulunmaktadır.

Makale detayları

Investigation of Methylation and Expression Levels in STK32C Gene in Oral Malignant Lesions

Yazarlar:

Seval Turna | Comert Sen | Kağan Avcı | Murat Ulusan | Nejat Vakur Olgaç | Semra Demokan

Yayıncı:

Giresun University

Ana Başlıklar:

Öngörücü ve Prognostik Belirteçler

Dergi:

Doğu Karadeniz Sağlık Bilimleri Dergisi Vol.4 Issue 3, pp.265-271

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001349985

pISSN:

2822-6445

Oral squamous cell carcinoma

methylation

expression

biomarkerOral skuamöz hücreli karsinom

metilasyon

ekspresyon

biyobelirteç

Sayı

Makale detayları

Yazarlar: Seval Turna | Comert Sen | Kağan Avcı | Murat Ulusan | Nejat Vakur Olgaç | Semra Demokan

Yayıncı: Giresun University

Ana Başlıklar: Öngörücü ve Prognostik Belirteçler

Dergi: Doğu Karadeniz Sağlık Bilimleri Dergisi Vol.4 Issue 3, pp.265-271

Belge Tipi: Makale

Yayın Tarihi: 9/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001349985

pISSN: 2822-6445

Sayı

Yazarlar:

Seval Turna | Comert Sen | Kağan Avcı | Murat Ulusan | Nejat Vakur Olgaç | Semra Demokan

Yayıncı:

Giresun University

Ana Başlıklar:

Öngörücü ve Prognostik Belirteçler

Dergi:

Doğu Karadeniz Sağlık Bilimleri Dergisi Vol.4 Issue 3, pp.265-271

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001349985

pISSN:

2822-6445