Evaluation of Mutagenicity of Torpedo EC Pesticide by Ames Salmonella/Microsome Method

Arzu Özkara

Makale detayları

Evaluation of Mutagenicity of Torpedo EC Pesticide by Ames Salmonella/Microsome Method

Yazarlar:

Arzu Özkara

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Yapısal Biyoloji

Dergi:

Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi Vol.25 Issue 5, pp.984-989

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001358486

pISSN:

2149-3367

Anahtar kelimeler:

Özet:

 In this study, the mutagenicity of Torpedo EC, a commercial form of abamectin, a pesticide from the Avermectin group, was evaluated using the AmesSalmonella microsome test. The test was performed in two stages on TA98 and TA100 strains of Salmonella typhimurium in the presence and absence of S9 fraction. In order to determine the doses to be applied, cytotoxic doses of the test substance were determined and 6 different non-cytotoxic test doses 1000, 800, 400, 200, 100, 50 μgplate were studied in triplicate. The obtained data were statistically evaluated using the Dunnett-t test and mutagenic activity was evaluated by comparing with the negative control group. According to the study results, it was determined that Torpedo EC at a dose of 1000 μgplate showed mutagenic activity on TA98 and TA100 strains both in the presence and absence of S9 fraction.

 In this study, the mutagenicity of Torpedo EC, a commercial form of abamectin, a pesticide from the Avermectin group, was evaluated using the AmesSalmonella microsome test. The test was performed in two stages on TA98 and TA100 strains of Salmonella typhimurium in the presence and absence of S9 fraction. In order to determine the doses to be applied, cytotoxic doses of the test substance were determined and 6 different non-cytotoxic test doses 1000, 800, 400, 200, 100, 50 μgplate were studied in triplicate. The obtained data were statistically evaluated using the Dunnett-t test and mutagenic activity was evaluated by comparing with the negative control group. According to the study results, it was determined that Torpedo EC at a dose of 1000 μgplate showed mutagenic activity on TA98 and TA100 strains both in the presence and absence of S9 fraction.

 In this study, the mutagenicity of Torpedo EC, a commercial form of abamectin, a pesticide from the Avermectin group, was evaluated using the AmesSalmonella microsome test. The test was performed in two stages on TA98 and TA100 strains of Salmonella typhimurium in the presence and absence of S9 fraction. In order to determine the doses to be applied, cytotoxic doses of the test substance were determined and 6 different non-cytotoxic test doses 1000, 800, 400, 200, 100, 50 μgplate were studied in triplicate. The obtained data were statistically evaluated using the Dunnett-t test and mutagenic activity was evaluated by comparing with the negative control group. According to the study results, it was determined that Torpedo EC at a dose of 1000 μgplate showed mutagenic activity on TA98 and TA100 strains both in the presence and absence of S9 fraction.

 Bu çalışmada avermectin grubu bir pestisit olan abamektinin ticari formu Torpedo ECnin AmesSalmonella mikrozom testi ile mutajenitesinin değerlendirilmesi gerçekleştirilmiştir. Test Salmonella typhimuriuma ait TA98 ve TA100 suşlarında S9 fraksiyonu varlığında ve yokluğunda iki aşamalı olarak yapılmıştır. Uygulanacak dozların belirlenmesi için test maddesinin sitotoksik dozları belirlenmiş ve deney için sitotoksik olmayan 6 farklı test dozu 1000, 800, 400, 200, 100, 50 μgplak üç tekrarlı olarak çalışılmıştır. Elde edilen veriler Dunnett-t testi kullanılarak istatistiksel açıdan değerlendirmeye alınmış ve negatif kontrol grubuyla karşılaştırılarak mutajenik aktivite değerlendirilmesi yapılmıştır. Çalışma sonucuna göre, Torpedo ECnin 1000 μgplak dozu hem S9 fraksiyonu varlığı hem de yokluğunda TA98 ve TA100 suşları üzerinde muatjenik aktivite gösterdiği tespit edilmiştir.

Makale detayları

Evaluation of Mutagenicity of Torpedo EC Pesticide by Ames Salmonella/Microsome Method

Yazarlar:

Arzu Özkara

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Yapısal Biyoloji

Dergi:

Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi Vol.25 Issue 5, pp.984-989

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001358486

pISSN:

2149-3367

Abamectin

Acaricide

Ames Test

mutagenicity

Torpedo ECAbamektin

Akarisit

Ames Test

Mutajenite

Torpedeo EC

Sayı

Makale detayları

Yazarlar: Arzu Özkara

Yayıncı: Afyon Kocatepe University

Ana Başlıklar: Yapısal Biyoloji

Dergi: Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi Vol.25 Issue 5, pp.984-989

Belge Tipi: Makale

Yayın Tarihi: 1/2025

Belge Dili: Türkçe

Document ID (Org No.): 2001358486

pISSN: 2149-3367

Sayı

Yazarlar:

Arzu Özkara

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Yapısal Biyoloji

Dergi:

Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi Vol.25 Issue 5, pp.984-989

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001358486

pISSN:

2149-3367