Saccharification of Hazelnut and Rhododendron Biomasses Using β-xylanase from Thermotoga naphthophila

Özgenur DİNÇER; Hasan Ufuk CELEBİOGLU; Attia HAMID; Muhammad Nauman AFTAB; Ahmet KARADAĞ

doi:https://doi.org/10.21597/jist.827308

Makale detayları

Saccharification of Hazelnut and Rhododendron Biomasses Using β-xylanase from Thermotoga naphthophila

Yazarlar:

Özgenur DİNÇER | Hasan Ufuk CELEBİOGLU | Attia HAMID | Muhammad Nauman AFTAB | Ahmet KARADAĞ

Yayıncı:

Igdir University

Ana Başlıklar:

Kimya mühendisliği

Dergi:

Journal of the Institute of Science and Technology 2021 Vol.11 Issue 2, pp.1321-1328

Belge Tipi:

Makale

Yayın Tarihi:

5/2021

Belge Dili:

İngilizce

Document ID (Org No.):

2000462898

Sıklık:

Üç Aylık

eISSN:

2536-4618

DOI:

https://doi.org/10.21597/jist.827308

Özet:

 

Enzymes can be used in various biotechnological applications due to the easy and cheap production. Since xylanase enzymes are preferred in various industries, researchon this enzyme is extensively being carried out. In this study, the β-xylanase gene was cloned from Thermotoga naphthophila, a thermophilic organism. The expression vector pET21a(+) was expressed in Escherichia coli BL21 (DE3). As a result of the studies, the pH, temperature and IPTG concentration of the enzyme were optimized to obtain highest expression. Dinitrosalicylic acid (DNS) was used to determine sugar content of the enzyme. The molecular mass of the
purified β-xylanase enzyme was determined using sodium dodecyl sulfate (SDS) polyacrylamide gel
electrophoresis. The molecular mass of the enzyme was calculated to be 38 kDa. Enzymatic hydrolysis of
hazelnut shell, rhododendron branch and rhododendron leaves was performed. Released reducing sugar contents from the enzymatic hydrolysis were calculated as 0.8461 mg mL-1, 0.6976 mg mL-1 and 0.3605 mg mL-1 for hazelnut shell, rhododendron branch, and rhododendron leaf respectively. In conclusion, β-xylanase enzyme can be an effective source for enzymatic hydrolysis to produce fermentable sugars from such biomasses.

 

Enzymes can be used in various biotechnological applications due to the easy and cheap production. Since xylanase enzymes are preferred in various industries, researchon this enzyme is extensively being carried out. In this study, the β-xylanase gene was cloned from Thermotoga naphthophila, a thermophilic organism. The expression vector pET21a(+) was expressed in Escherichia coli BL21 (DE3). As a result of the studies, the pH, temperature and IPTG concentration of the enzyme were optimized to obtain highest expression. Dinitrosalicylic acid (DNS) was used to determine sugar content of the enzyme. The molecular mass of the
purified β-xylanase enzyme was determined using sodium dodecyl sulfate (SDS) polyacrylamide gel
electrophoresis. The molecular mass of the enzyme was calculated to be 38 kDa. Enzymatic hydrolysis of
hazelnut shell, rhododendron branch and rhododendron leaves was performed. Released reducing sugar contents from the enzymatic hydrolysis were calculated as 0.8461 mg mL-1, 0.6976 mg mL-1 and 0.3605 mg mL-1 for hazelnut shell, rhododendron branch, and rhododendron leaf respectively. In conclusion, β-xylanase enzyme can be an effective source for enzymatic hydrolysis to produce fermentable sugars from such biomasses.

Makale detayları

Saccharification of Hazelnut and Rhododendron Biomasses Using β-xylanase from Thermotoga naphthophila

Yazarlar: Özgenur DİNÇER | Hasan Ufuk CELEBİOGLU | Attia HAMID | Muhammad Nauman AFTAB | Ahmet KARADAĞ

Yayıncı: Igdir University

Ana Başlıklar: Kimya mühendisliği

Dergi: Journal of the Institute of Science and Technology 2021 Vol.11 Issue 2, pp.1321-1328

Belge Tipi: Makale

Yayın Tarihi: 5/2021

Belge Dili: İngilizce

Document ID (Org No.): 2000462898

Sıklık: Üç Aylık

eISSN: 2536-4618

DOI: https://doi.org/10.21597/jist.827308

Sayı

Yazarlar:

Özgenur DİNÇER | Hasan Ufuk CELEBİOGLU | Attia HAMID | Muhammad Nauman AFTAB | Ahmet KARADAĞ

Yayıncı:

Igdir University

Ana Başlıklar:

Kimya mühendisliği

Dergi:

Journal of the Institute of Science and Technology 2021 Vol.11 Issue 2, pp.1321-1328

Belge Tipi:

Makale

Yayın Tarihi:

5/2021

Belge Dili:

İngilizce

Document ID (Org No.):

2000462898

Sıklık:

Üç Aylık

eISSN:

2536-4618

DOI:

https://doi.org/10.21597/jist.827308