Investigation of the Possible Role of Macrophage Migration Inhibitory Factor Gene -173G/C Polymorphism ın Patients with Atherosclerosis

Rojda Tanrıverdi; Şenay Balcı; Merve Türkegün Şengül; Ahmet Çelik; Lülüfer Tamer

Makale detayları

Investigation of the Possible Role of Macrophage Migration Inhibitory Factor Gene -173G/C Polymorphism ın Patients with Atherosclerosis

Yazarlar:

Rojda Tanrıverdi | Şenay Balcı | Merve Türkegün Şengül | Ahmet Çelik | Lülüfer Tamer

Yayıncı:

Kirsehir Ahi Evran University

Ana Başlıklar:

Klinik Tıp Bilimleri | Klinik Tıp Bilimleri (Diğer)

Dergi:

Ahi Evran Tıp Dergisi Vol.7 Issue 2, pp.171-176

Belge Tipi:

Makale

Yayın Tarihi:

7/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001291841

pISSN:

2619-9203

Anahtar kelimeler:

Özet:

 Purpose: Atherosclerosis is defined as an inflammatory disease that results in the formation of atherosclerotic plaques caused by the deposition of cholesterol in the arterial intima. As a result of damage to the intima layer of the vessel, foam cell formation following cholesterol accumulation and plaque development due to smooth muscle cell increase are observed. In different stages of atherosclerosis, migration of leukocytes to the vessel wall is provided by functional type chemokines; ıt was aimed to investigate the possible role of MIF -173GC polymorphism in atherosclerosis disease depending on this function, since it has the same functional properties as chemokines. Materials and Methods: Thirty patients with 70 occlusion detected by angiography and 30 healthy individuals were included in the study. DNA isolation was performed with DNA isolation kit from blood samples taken into EDTA tubes from individuals participating in the study. Analysis of MIF -173GC polymorphism was performed on Real Time PCR LC480, Roche. Statistical analyzes were performed with the program STATISTICA version 13.5.0.17 TIBCO Software Inc. 2017. Statistical significance level p 0.05 was taken in all comparisons. Results: The frequency of GG, GC and CC genotypes in the MIF -173GC polymorphism was 55.26, 41.18 and 66.66 in the patient group, respectively, and 44.74, 58.82 and 33.33 in the control group. When compared with the GG genotype, it was determined that those with the GC genotype had a 0.567-fold p0.3367 risk of developing the disease and those with the CC genotype had a 1.6190-fold p 0.7038 risk of developing the disease. Conclusion: It was determined that those with the C allele in the MIF-173 GC polymorphism pose a risk for atherosclerosis disease.

 Purpose: Atherosclerosis is defined as an inflammatory disease that results in the formation of atherosclerotic plaques caused by the deposition of cholesterol in the arterial intima. As a result of damage to the intima layer of the vessel, foam cell formation following cholesterol accumulation and plaque development due to smooth muscle cell increase are observed. In different stages of atherosclerosis, migration of leukocytes to the vessel wall is provided by functional type chemokines; ıt was aimed to investigate the possible role of MIF -173GC polymorphism in atherosclerosis disease depending on this function, since it has the same functional properties as chemokines. Materials and Methods: Thirty patients with 70 occlusion detected by angiography and 30 healthy individuals were included in the study. DNA isolation was performed with DNA isolation kit from blood samples taken into EDTA tubes from individuals participating in the study. Analysis of MIF -173GC polymorphism was performed on Real Time PCR LC480, Roche. Statistical analyzes were performed with the program STATISTICA version 13.5.0.17 TIBCO Software Inc. 2017. Statistical significance level p 0.05 was taken in all comparisons. Results: The frequency of GG, GC and CC genotypes in the MIF -173GC polymorphism was 55.26, 41.18 and 66.66 in the patient group, respectively, and 44.74, 58.82 and 33.33 in the control group. When compared with the GG genotype, it was determined that those with the GC genotype had a 0.567-fold p0.3367 risk of developing the disease and those with the CC genotype had a 1.6190-fold p 0.7038 risk of developing the disease. Conclusion: It was determined that those with the C allele in the MIF-173 GC polymorphism pose a risk for atherosclerosis disease.

 Purpose: Atherosclerosis is defined as an inflammatory disease that results in the formation of atherosclerotic plaques caused by the deposition of cholesterol in the arterial intima. As a result of damage to the intima layer of the vessel, foam cell formation following cholesterol accumulation and plaque development due to smooth muscle cell increase are observed. In different stages of atherosclerosis, migration of leukocytes to the vessel wall is provided by functional type chemokines; ıt was aimed to investigate the possible role of MIF -173GC polymorphism in atherosclerosis disease depending on this function, since it has the same functional properties as chemokines. Materials and Methods: Thirty patients with 70 occlusion detected by angiography and 30 healthy individuals were included in the study. DNA isolation was performed with DNA isolation kit from blood samples taken into EDTA tubes from individuals participating in the study. Analysis of MIF -173GC polymorphism was performed on Real Time PCR LC480, Roche. Statistical analyzes were performed with the program STATISTICA version 13.5.0.17 TIBCO Software Inc. 2017. Statistical significance level p 0.05 was taken in all comparisons. Results: The frequency of GG, GC and CC genotypes in the MIF -173GC polymorphism was 55.26, 41.18 and 66.66 in the patient group, respectively, and 44.74, 58.82 and 33.33 in the control group. When compared with the GG genotype, it was determined that those with the GC genotype had a 0.567-fold p0.3367 risk of developing the disease and those with the CC genotype had a 1.6190-fold p 0.7038 risk of developing the disease. Conclusion: It was determined that those with the C allele in the MIF-173 GC polymorphism pose a risk for atherosclerosis disease.

 Amaç: Ateroskleroz, arteriyel intimada kolesterol birikiminin neden olduğu aterosklerotik plak oluşumu ile sonuçlanan enflamatuar bir hastalık olarak tanımlanmaktadır. Damarın intima tabakasındaki hasarlanma sonucunda kolesterol birikmesini takiben köpük hücre oluşumu ve düz kas hücre artışına bağlı olarak plak gelişimi görülmektedir. Aterosklerozun farklı aşamalarında, lökositlerin damar duvarına göçü fonksiyonel türdeki kemokinler ile sağlanmaktadır; kemokinlerle aynı fonksiyonel özelliklere sahip olduğu için MIF -173GC polimorfizminin bu fonksiyona bağlı olarak ateroskleroz hastalığındaki olası rolünün araştırılması amaçlanmıştır. Araçlar ve Yöntem: Çalışmaya, anjiyografi ile 70 tıkanıklık tespit edilen 30 hasta ve 30 sağlıklı birey dahil edildi. Çalışmaya katılan bireylerden EDTAlı tüplere alınan kan örneklerinden DNA izolasyon kiti ile DNA izolasyonu yapıldı. MIF -173GC polimorfizminin analizi Real Time PCR LC480, Roche cihazında gerçekleştirildi. İstatistiksel analizler STATISTICA version 13.5.0.17 TIBCO Software Inc. 2017 programı ile yapıldı. Tüm karşılaştırmalarda istatistik önem seviyesi p 0.05 alınmıştır. Bulgular: MIF -173GC polimorfizminde, hasta grubunun GG, GC ve CC genotipi sıklığı sırasıyla 55.26 41.18 ve 66.66 kontrol grubunda ise 44.74 58.82 ve 33.33 olarak saptandı. GG genotipiyle karşılaştırıldığında, GC genotipine sahip olanlar 0.567 kat p0.3367, CC genotipine sahip olanlar 1.6190 kat p0.7038 hastalık geliştirme riskine sahip olduğu belirlendi. Sonuç: MIF -173 GC polimorfizminde C aleline sahip olanların ateroskleroz hastalığı için risk oluşturduğu saptandı.

Makale detayları

Investigation of the Possible Role of Macrophage Migration Inhibitory Factor Gene -173G/C Polymorphism ın Patients with Atherosclerosis

Yazarlar:

Rojda Tanrıverdi | Şenay Balcı | Merve Türkegün Şengül | Ahmet Çelik | Lülüfer Tamer

Yayıncı:

Kirsehir Ahi Evran University

Ana Başlıklar:

Klinik Tıp Bilimleri | Klinik Tıp Bilimleri (Diğer)

Dergi:

Ahi Evran Tıp Dergisi Vol.7 Issue 2, pp.171-176

Belge Tipi:

Makale

Yayın Tarihi:

7/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001291841

pISSN:

2619-9203

MIF

PCR

ATEROSCLEROSISMIF

PCR

ATEROSKLEROZ

Sayı

Makale detayları

Investigation of the Possible Role of Macrophage Migration Inhibitory Factor Gene -173G/C Polymorphism ın Patients with Atherosclerosis

Yazarlar: Rojda Tanrıverdi | Şenay Balcı | Merve Türkegün Şengül | Ahmet Çelik | Lülüfer Tamer

Yayıncı: Kirsehir Ahi Evran University

Ana Başlıklar: Klinik Tıp Bilimleri | Klinik Tıp Bilimleri (Diğer)

Dergi: Ahi Evran Tıp Dergisi Vol.7 Issue 2, pp.171-176

Belge Tipi: Makale

Yayın Tarihi: 7/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001291841

pISSN: 2619-9203

MIF

PCR

ATEROSCLEROSISMIF

PCR

ATEROSKLEROZ

Sayı

Yazarlar:

Rojda Tanrıverdi | Şenay Balcı | Merve Türkegün Şengül | Ahmet Çelik | Lülüfer Tamer

Yayıncı:

Kirsehir Ahi Evran University

Ana Başlıklar:

Klinik Tıp Bilimleri | Klinik Tıp Bilimleri (Diğer)

Dergi:

Ahi Evran Tıp Dergisi Vol.7 Issue 2, pp.171-176

Belge Tipi:

Makale

Yayın Tarihi:

7/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001291841

pISSN:

2619-9203