Stimulation of the production of hypericins in in vitro seedlings of Hypericum adenotrichum by some biotic elicitors

Ömer YAMANER; Bengi ERDAĞ; Cengiz GÖKBULUT

Makale detayları

Stimulation of the production of hypericins in in vitro seedlings of Hypericum adenotrichum by some biotic elicitors

Yazarlar:

Ömer YAMANER | Bengi ERDAĞ | Cengiz GÖKBULUT

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Botany 2013 Vol.37 Issue 1, pp.153-159

Belge Tipi:

Makale

Yayın Tarihi:

12/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000722852

Sıklık:

İki Aylık

eISSN:

1303-6106

pISSN:

1300-008X

Özet:

 

The goal of this work was to study the stimulating effects of mannan and pectin on the biosynthesis of hypericins in in vitro seedlings of Hypericum adenotrichum Spach. Seedlings of H. adenotrichum were grown on a modified MS medium containing mannan (10, 50, and 100 mg/L) or pectin (10, 50, and 100 mg/L) for 15 and 30 days. Seedlings of H. adenotrichum were extracted with methanol. The methanol extracts of the seedlings were analysed by HPLC to investigate the changes in hypericin and pseudohypericin levels. The best results were obtained by exposure to 50 mg/L mannan or pectin for 15 days. Mannan stimulated pseudohypericin production up to 2.8-fold and hypericin production up to 1.7-fold. Pectin stimulated pseudohypericin production up to 4.8-fold and hypericin production up to 2.7-fold. The increases in hypericins in response to mannan and pectin demonstrate that these elicitors can be evaluated for production of secondary metabolites in Hypericum species.

 

The goal of this work was to study the stimulating effects of mannan and pectin on the biosynthesis of hypericins in in vitro seedlings of Hypericum adenotrichum Spach. Seedlings of H. adenotrichum were grown on a modified MS medium containing mannan (10, 50, and 100 mg/L) or pectin (10, 50, and 100 mg/L) for 15 and 30 days. Seedlings of H. adenotrichum were extracted with methanol. The methanol extracts of the seedlings were analysed by HPLC to investigate the changes in hypericin and pseudohypericin levels. The best results were obtained by exposure to 50 mg/L mannan or pectin for 15 days. Mannan stimulated pseudohypericin production up to 2.8-fold and hypericin production up to 1.7-fold. Pectin stimulated pseudohypericin production up to 4.8-fold and hypericin production up to 2.7-fold. The increases in hypericins in response to mannan and pectin demonstrate that these elicitors can be evaluated for production of secondary metabolites in Hypericum species.

Makale detayları

Stimulation of the production of hypericins in in vitro seedlings of Hypericum adenotrichum by some biotic elicitors

Yazarlar: Ömer YAMANER | Bengi ERDAĞ | Cengiz GÖKBULUT

Yayıncı: TUBITAK

Dergi: Turkish Journal of Botany 2013 Vol.37 Issue 1, pp.153-159

Belge Tipi: Makale

Yayın Tarihi: 12/2012

Belge Dili: Türkçe

Document ID (Org No.): 2000722852

Sıklık: İki Aylık

eISSN: 1303-6106

pISSN: 1300-008X

Sayı

Yazarlar:

Ömer YAMANER | Bengi ERDAĞ | Cengiz GÖKBULUT

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Botany 2013 Vol.37 Issue 1, pp.153-159

Belge Tipi:

Makale

Yayın Tarihi:

12/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000722852

Sıklık:

İki Aylık

eISSN:

1303-6106

pISSN:

1300-008X