IN VITRO EVALUATION OF THE EFFECT OF GLUTATHIONE ON CASPASE SYSTEM AND OXIDATIVE DNA DAMAGE IN HIGH GLUCOSE CONDITION

Gülay YAYCI; Semiha DEDE; Ayşe USTA; Veysel YÜKSEK; Sedat ÇETİN

doi:https://doi.org/10.18036/estubtdc.802794

Makale detayları

IN VITRO EVALUATION OF THE EFFECT OF GLUTATHIONE ON CASPASE SYSTEM AND OXIDATIVE DNA DAMAGE IN HIGH GLUCOSE CONDITION

Yazarlar:

Gülay YAYCI | Semiha DEDE | Ayşe USTA | Veysel YÜKSEK | Sedat ÇETİN

Yayıncı:

Eskisehir Technical University

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 2021 Vol.10 Issue 2, pp.132-137

Belge Tipi:

Makale

Yayın Tarihi:

7/2021

Belge Dili:

İngilizce

Document ID (Org No.):

2000417405

pISSN:

2667-4203

DOI:

https://doi.org/10.18036/estubtdc.802794

Özet:

 

This study was planned to investigate the effects of glutathione, known to be a strong antioxidant, on caspase-dependent apoptosis and oxidative DNA damage in the kidney cells (BHK-21) exposed to high glucose. BHK-21 cell line was cultivated by regular passages in vitro conditions. Study groups were set up as control, study and its combinations groups ((Glucose; (285 mM, HG), glutathione (250 µM)). After 24 hours of incubation, trypsinized cells were disrupted by freeze-thaw method and analyte was prepared. Caspase 3, 8, 9, M30 and 8-OHdG (oxidative DNA damage marker) levels were determined using commercial ELISA kits. M30, caspase 3, 8 and 9 which are the parameters of apoptosis, were found highest in the HG group. In GSH-treated groups, these parameters decreased slightly. There was no significant difference in the levels of 8-OHdG which is the indicator of oxidative DNA damage.

 

This study was planned to investigate the effects of glutathione, known to be a strong antioxidant, on caspase-dependent apoptosis and oxidative DNA damage in the kidney cells (BHK-21) exposed to high glucose. BHK-21 cell line was cultivated by regular passages in vitro conditions. Study groups were set up as control, study and its combinations groups ((Glucose; (285 mM, HG), glutathione (250 µM)). After 24 hours of incubation, trypsinized cells were disrupted by freeze-thaw method and analyte was prepared. Caspase 3, 8, 9, M30 and 8-OHdG (oxidative DNA damage marker) levels were determined using commercial ELISA kits. M30, caspase 3, 8 and 9 which are the parameters of apoptosis, were found highest in the HG group. In GSH-treated groups, these parameters decreased slightly. There was no significant difference in the levels of 8-OHdG which is the indicator of oxidative DNA damage.

Makale detayları

IN VITRO EVALUATION OF THE EFFECT OF GLUTATHIONE ON CASPASE SYSTEM AND OXIDATIVE DNA DAMAGE IN HIGH GLUCOSE CONDITION

Yazarlar: Gülay YAYCI | Semiha DEDE | Ayşe USTA | Veysel YÜKSEK | Sedat ÇETİN

Yayıncı: Eskisehir Technical University

Ana Başlıklar: Yaşam bilimleri

Dergi: Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 2021 Vol.10 Issue 2, pp.132-137

Belge Tipi: Makale

Yayın Tarihi: 7/2021

Belge Dili: İngilizce

Document ID (Org No.): 2000417405

pISSN: 2667-4203

DOI: https://doi.org/10.18036/estubtdc.802794

Sayı

Yazarlar:

Gülay YAYCI | Semiha DEDE | Ayşe USTA | Veysel YÜKSEK | Sedat ÇETİN

Yayıncı:

Eskisehir Technical University

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 2021 Vol.10 Issue 2, pp.132-137

Belge Tipi:

Makale

Yayın Tarihi:

7/2021

Belge Dili:

İngilizce

Document ID (Org No.):

2000417405

pISSN:

2667-4203

DOI:

https://doi.org/10.18036/estubtdc.802794