Isolation and characterization of alkane hydrocarbons-degrading Delftia tsuruhatensis strain D9 from petroleum-contaminated soils

Ayşe EREN; Kemal GÜVEN

doi:https://doi.org/10.38042/biotechstudies.1103695

Makale detayları

Isolation and characterization of alkane hydrocarbons-degrading Delftia tsuruhatensis strain D9 from petroleum-contaminated soils

Yazarlar:

Ayşe EREN | Kemal GÜVEN

Yayıncı:

Tarla Bitkileri Merkez Araştırma Enstitüsü

Ana Başlıklar:

Genetik ve evrim

Dergi:

Biotech Studies 2022 Vol.31 Issue 1, pp.36-44

Belge Tipi:

Makale

Yayın Tarihi:

6/2022

Belge Dili:

İngilizce

Document ID (Org No.):

2000904885

Sıklık:

Aylık

eISSN:

2757-5233

pISSN:

2687-3761

DOI:

https://doi.org/10.38042/biotechstudies.1103695

Özet:

 

A bacterial strain from petroleum-contaminated soil in south-eastern Turkey was isolated and characterized to determine the potential of alkane hydrocarbon biodegradation. Phenotypic characteristics and the sequence analysis of the 16S rRNA gene revealed that the strain D9 is a member of the Delfitia genus and most similar to Delftia tsuruhatensis (100%). The optimum pH and temperature values for the growth of D. tsuruhatensis strain D9 were found to be 9.0-10.0 and 35°C, respectively. The strain was found to grow in some single, medium and long-chain hydrocarbons such as decane, hexadecane, and squalene, tested by short-time incubation in basal medium (BM) in the presence of 1% hydrocarbon concentrations under optimum conditions. After incubation for 3 days, 65% of the single hydrocarbon hexadecane was degraded by the D. tsuruhatensis strain D9, revealed by GC-MS analysis. The biodegradation of petroleum hydrocarbons by D. tsuruhatensis strain D9 isolated and characterized in the present study shows that it can be a good candidate in the bioremediation process.

Makale detayları

Isolation and characterization of alkane hydrocarbons-degrading Delftia tsuruhatensis strain D9 from petroleum-contaminated soils

Yazarlar: Ayşe EREN | Kemal GÜVEN

Yayıncı: Tarla Bitkileri Merkez Araştırma Enstitüsü

Ana Başlıklar: Genetik ve evrim

Dergi: Biotech Studies 2022 Vol.31 Issue 1, pp.36-44

Belge Tipi: Makale

Yayın Tarihi: 6/2022

Belge Dili: İngilizce

Document ID (Org No.): 2000904885

Sıklık: Aylık

eISSN: 2757-5233

pISSN: 2687-3761

DOI: https://doi.org/10.38042/biotechstudies.1103695

Sayı

Yazarlar:

Ayşe EREN | Kemal GÜVEN

Yayıncı:

Tarla Bitkileri Merkez Araştırma Enstitüsü

Ana Başlıklar:

Genetik ve evrim

Dergi:

Biotech Studies 2022 Vol.31 Issue 1, pp.36-44

Belge Tipi:

Makale

Yayın Tarihi:

6/2022

Belge Dili:

İngilizce

Document ID (Org No.):

2000904885

Sıklık:

Aylık

eISSN:

2757-5233

pISSN:

2687-3761

DOI:

https://doi.org/10.38042/biotechstudies.1103695