Localization of plasminogen activator inhibitor type 1 and 2 in preimplantation mouse development in vitro

Duygu MUTLUAY; Yukiko YAMAZAKİ; Kanani HOKUTAN; Charles J ROSSER; Hideki FURUYA

doi:https://doi.org/10.33988/auvfd.533634

Makale detayları

Localization of plasminogen activator inhibitor type 1 and 2 in preimplantation mouse development in vitro

Yazarlar:

Duygu MUTLUAY | Yukiko YAMAZAKİ | Kanani HOKUTAN | Charles J ROSSER | Hideki FURUYA

Ana Başlıklar:

Hayvancılık

Dergi:

Ankara Üniversitesi Veteriner Fakültesi Dergisi 2019 Vol.66 Issue 4, pp.357-361

Belge Tipi:

Makale

Yayın Tarihi:

9/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000849580

DOI:

https://doi.org/10.33988/auvfd.533634

Özet:

 


Plasminogen activator
inhibitor type 1 (PAI-1) and type 2 (PAI-2) are the major endogenous inhibitors
of fibrinolysis, or thrombolysis, as it is effective in blocking the conversion
of plasminogen to plasmin. In mammalian embryos, both PAI-1 and PAI-2 proteins
are expressed in the trophoblasts during and after implantation, suggesting
their critical roles in implantation and placentation during pregnancy.
However, it remains unclear how both proteins localize in the early stage
embryos before implantation. In this
study, 2 cell stage embryos were flushed from the oviducts and cultured to
specified stages in medium at 37 ̊C in a 5% CO2 incubator. Embryos
were fixed and double immunostained with anti-PAI-1 and anti- PAI-2 antibody.
We determined the critical expression and localization patterns of PAI-1 and
PAI-2 proteins in murine preimplantation embryos at 2 cell, 8 cell, morula and
blastocyst stages by using confocal laser scanning microscope. We found that
PAI-1 and PAI-2 constantly express in the embryos during preimplantation
development, and these proteins localize in both the cytoplasm and the nucleus
of each blastomere regardless of their developmental stage. Our results suggest
that PAI-1 and PAI-2 proteins may play roles in
early embryonic development before implantation.


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Makale detayları

Localization of plasminogen activator inhibitor type 1 and 2 in preimplantation mouse development in vitro

Yazarlar: Duygu MUTLUAY | Yukiko YAMAZAKİ | Kanani HOKUTAN | Charles J ROSSER | Hideki FURUYA

Ana Başlıklar: Hayvancılık

Dergi: Ankara Üniversitesi Veteriner Fakültesi Dergisi 2019 Vol.66 Issue 4, pp.357-361

Belge Tipi: Makale

Yayın Tarihi: 9/2019

Belge Dili: İngilizce

Document ID (Org No.): 2000849580

DOI: https://doi.org/10.33988/auvfd.533634

Sayı

Yazarlar:

Duygu MUTLUAY | Yukiko YAMAZAKİ | Kanani HOKUTAN | Charles J ROSSER | Hideki FURUYA

Ana Başlıklar:

Hayvancılık

Dergi:

Ankara Üniversitesi Veteriner Fakültesi Dergisi 2019 Vol.66 Issue 4, pp.357-361

Belge Tipi:

Makale

Yayın Tarihi:

9/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000849580

DOI:

https://doi.org/10.33988/auvfd.533634