DIFFERENT APPROACHES FOR BREAST CANCER: VOLTAGE GATED  POTASSIUM CHANNELS AND MICRORNAS

Çağrı ÖNER; Ertuğrul ÇOLAK; Didem TURGUT COŞAN

doi:https://doi.org/10.1501/commuc_0000000181

Makale detayları

DIFFERENT APPROACHES FOR BREAST CANCER: VOLTAGE GATED POTASSIUM CHANNELS AND MICRORNAS

Yazarlar:

Çağrı ÖNER | Ertuğrul ÇOLAK | Didem TURGUT COŞAN

Yayıncı:

Ankara University

Dergi:

Communications Faculty of Sciences University of Ankara Series C Biology 2015 Vol.24 Issue 1, pp.1-17

Belge Tipi:

Makale

Yayın Tarihi:

5/2015

Belge Dili:

İngilizce

Document ID (Org No.):

2000911345

Sıklık:

Aylık

eISSN:

2651-3749

pISSN:

1303-6025

DOI:

https://doi.org/10.1501/commuc_0000000181

Özet:

 

Micro RNAs and voltage-gated potassium channels (VGPCs) both play critical roles in the development of cancer. We aimed to reveal the diversity of miR-126/126*, which effects angiogenesis and vascular development through the inhibition of VGPCs. In this study, potassium channel inhibitors, including tetraethylammonium (5mM), 4-aminopyridine (5mM), margatoxin (1µM) and astemizole (2µM), were applied to MCF-7 and MDA-MB-231 breast cancer cell lines. After totally isolating RNA from the cells, Real-Time Polymerase Chain Reaction was used in order to identify gene expressions. OneWay ANOVA was used for variation analyses, while Tukey HSD and Tamhane were used to assess whether multiple comparisons were statistically significant (P < 0.001). Our results showed an increase in miR-126/126* expressions after the channel inhibition of MCF-7 and MDA-MB-231 cell lines (P < 0.001). miR-126/126* expressions were increased using TEA, 4-AP and astemizole in both cell lines. miR-126/126* expressions were only increased through the use of margatoxin in MCF-7. miR-126/126* may interact with voltage-gated potassium channels. In our study, the inhibition of K channels using K channel blockers resulted in an increase of miR-126/126* expression. Therefore, our data suggested that there could be another perspective between K channels and non-coding RNAs in the development of breast cancer.

Makale detayları

DIFFERENT APPROACHES FOR BREAST CANCER: VOLTAGE GATED POTASSIUM CHANNELS AND MICRORNAS

Yazarlar: Çağrı ÖNER | Ertuğrul ÇOLAK | Didem TURGUT COŞAN

Yayıncı: Ankara University

Dergi: Communications Faculty of Sciences University of Ankara Series C Biology 2015 Vol.24 Issue 1, pp.1-17

Belge Tipi: Makale

Yayın Tarihi: 5/2015

Belge Dili: İngilizce

Document ID (Org No.): 2000911345

Sıklık: Aylık

eISSN: 2651-3749

pISSN: 1303-6025

DOI: https://doi.org/10.1501/commuc_0000000181

Sayı

Yazarlar:

Çağrı ÖNER | Ertuğrul ÇOLAK | Didem TURGUT COŞAN

Yayıncı:

Ankara University

Dergi:

Communications Faculty of Sciences University of Ankara Series C Biology 2015 Vol.24 Issue 1, pp.1-17

Belge Tipi:

Makale

Yayın Tarihi:

5/2015

Belge Dili:

İngilizce

Document ID (Org No.):

2000911345

Sıklık:

Aylık

eISSN:

2651-3749

pISSN:

1303-6025

DOI:

https://doi.org/10.1501/commuc_0000000181