Polyphenolic contents of Teucrium polium L. and Teucrium scordium L. associated with their protective effects against MMC-induced chromosomal damage in cultured human peripheral blood lymphocytes

Olivera MILOŠEVIĆ-DJORDJEVIĆ; Marina ADOVIĆ JAKOVLJEVIĆ; Aleksandra MARKOVIĆ; Milan STANKOVIĆ; Andrija ĆIRIĆ; Dragoslav MARINKOVIĆ; Darko GRUJIČIĆ

Makale detayları

Polyphenolic contents of Teucrium polium L. and Teucrium scordium L. associated with their protective effects against MMC-induced chromosomal damage in cultured human peripheral blood lymphocytes

Yazarlar:

Olivera MILOŠEVIĆ-DJORDJEVIĆ | Marina ADOVIĆ JAKOVLJEVIĆ | Aleksandra MARKOVIĆ | Milan STANKOVIĆ | Andrija ĆIRIĆ | Dragoslav MARINKOVIĆ | Darko GRUJIČIĆ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2018 Vol.42 Issue 2, pp.152-162

Belge Tipi:

Makale

Yayın Tarihi:

4/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000811243

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Özet:

 

Teucrium
  species  have  been  used  in  traditional  medicine  for  treatment  of  different  diseases.  The  aim  of  this  study  was  to  
investigate polyphenolic contents by high-performance liquid chromatography (HPLC), and the genotoxic effect of methanolic extracts 
of 
Teucrium  polium 
and
  Teucrium  scordium  
using  the  cytokinesis-block  micronucleus  (CBMN)  assay  on  human  peripheral  blood  
lymphocytes  (PBLs)  from  healthy  donors.  The  HPLC  analysis  showed  that  extracts  consist  of  phenolic  acid  (gallic,  vanillic,  caffeic,  
chlorogenic, p-coumaric, sinapic) and flavonoids (catechin, rutin, myricetin, luteolin, quercetin and apigenin). Cultures were treated 
with  extracts  of  both  plants  separately  and  in  combinations  with  mitomycin  C  (MMC).  In  separate  treatments,  both  herbal  extracts  
significantly induced micronucleus (MN) frequency only at the highest concentrations. All concentrations of 
T.  scordium
, except the 
lowest,  and  all  concentrations  of  
T.  polium
  extracts  in  combined  treatment  with  MMC  significantly  reduced  the  frequency  of  MN.  
The  extract  of  
T.
polium
  did  not  significantly  affect  the  nuclear  division  index  (NDI),  whereas  
T.  scordium
  in  higher  concentrations,  
separately and in combined treatment with MMC, significantly decreased the NDI value. Our results suggest that both herbal extracts in 
combination with MMC have antimutagenic (
T.
polium) 
and
proapoptotic effects
(
T. scordium
), which indicates their protective effects 
in PBLs.

Makale detayları

Polyphenolic contents of <i>Teucrium polium</i> L. and <i>Teucrium scordium</i> L. associated with their protective effects against MMC-induced chromosomal damage in cultured human peripheral blood lymphocytes

Yazarlar:

Olivera MILOŠEVIĆ-DJORDJEVIĆ | Marina ADOVIĆ JAKOVLJEVIĆ | Aleksandra MARKOVIĆ | Milan STANKOVIĆ | Andrija ĆIRIĆ | Dragoslav MARINKOVIĆ | Darko GRUJIČIĆ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2018 Vol.42 Issue 2, pp.152-162

Belge Tipi:

Makale

Yayın Tarihi:

4/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000811243

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Sayı

Makale detayları

Polyphenolic contents of <i>Teucrium polium</i> L. and <i>Teucrium scordium</i> L. associated with their protective effects against MMC-induced chromosomal damage in cultured human peripheral blood lymphocytes

Yazarlar: Olivera MILOŠEVIĆ-DJORDJEVIĆ | Marina ADOVIĆ JAKOVLJEVIĆ | Aleksandra MARKOVIĆ | Milan STANKOVIĆ | Andrija ĆIRIĆ | Dragoslav MARINKOVIĆ | Darko GRUJIČIĆ

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2018 Vol.42 Issue 2, pp.152-162

Belge Tipi: Makale

Yayın Tarihi: 4/2018

Belge Dili: İngilizce

Document ID (Org No.): 2000811243

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Sayı

Yazarlar:

Olivera MILOŠEVIĆ-DJORDJEVIĆ | Marina ADOVIĆ JAKOVLJEVIĆ | Aleksandra MARKOVIĆ | Milan STANKOVIĆ | Andrija ĆIRIĆ | Dragoslav MARINKOVIĆ | Darko GRUJIČIĆ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2018 Vol.42 Issue 2, pp.152-162

Belge Tipi:

Makale

Yayın Tarihi:

4/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000811243

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152