Artichoke compound cynarin differentially affects the survival, growth, and stress response of normal, immortalized, and cancerous human cells

CEREN GEZER; SEVİNÇ YÜCECAN; SURESH INDER SINGH RATTAN

doi:https://doi.org/10.3906/biy-1407-67

Makale detayları

Artichoke compound cynarin differentially affects the survival, growth, and stress response of normal, immortalized, and cancerous human cells

Yazarlar:

CEREN GEZER | SEVİNÇ YÜCECAN | SURESH INDER SINGH RATTAN

Yayıncı:

TUBITAK

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 2

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

İngilizce

Document ID (Org No.):

2000811990

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

DOI:

https://doi.org/10.3906/biy-1407-67

Anahtar kelimeler:

Özet:

 

Cynarin (CYN) is the main derivative of caffeoylquinic acid, found in leaves and heads of artichoke. It may have hepatoprotective, antiatherosclerotic, antioxidative, choleretic, and cholesterol-lowering effects. We tested the effects of various doses of CYN on the proliferative potential, survival, morphology, and stress response (SR) markers heme oxygenase-1 (HO-1) and heat shock protein-70 (HSP70) in normal human skin fibroblasts (FSF-1), telomerase-immortalized mesenchymal stem cells (hTERT-MSC), and cervical cancer cells (HeLa). The effects of CYN on cell proliferation and morphology were dose- and cell type-dependent, with 500 µM CYN as the upper limit for all cell types. While the growth and proliferation of cells decreased after exposure to 75 µM CYN for 3 days, overall survival of FSF-1 and hTERT-MSC was higher than that of HeLa cells. Furthermore, CYN induced the oxidative SR marker HO-1 in both fibroblasts and stem cells in a biphasic manner. A slight induction of HSP70 was observed only in the stem cells. Thus, CYN may be useful for protection against the growth and survival of potentially cancerous cells and may promote longevity of normal cells by inducing SR proteins. Further advanced research related to CYN and artichoke is recommended.

 

Cynarin (CYN) is the main derivative of caffeoylquinic acid, found in leaves and heads of artichoke. It may have hepatoprotective, antiatherosclerotic, antioxidative, choleretic, and cholesterol-lowering effects. We tested the effects of various doses of CYN on the proliferative potential, survival, morphology, and stress response (SR) markers heme oxygenase-1 (HO-1) and heat shock protein-70 (HSP70) in normal human skin fibroblasts (FSF-1), telomerase-immortalized mesenchymal stem cells (hTERT-MSC), and cervical cancer cells (HeLa). The effects of CYN on cell proliferation and morphology were dose- and cell type-dependent, with 500 µM CYN as the upper limit for all cell types. While the growth and proliferation of cells decreased after exposure to 75 µM CYN for 3 days, overall survival of FSF-1 and hTERT-MSC was higher than that of HeLa cells. Furthermore, CYN induced the oxidative SR marker HO-1 in both fibroblasts and stem cells in a biphasic manner. A slight induction of HSP70 was observed only in the stem cells. Thus, CYN may be useful for protection against the growth and survival of potentially cancerous cells and may promote longevity of normal cells by inducing SR proteins. Further advanced research related to CYN and artichoke is recommended.

Makale detayları

Artichoke compound cynarin differentially affects the survival, growth, and stress response of normal, immortalized, and cancerous human cells

Yazarlar:

CEREN GEZER | SEVİNÇ YÜCECAN | SURESH INDER SINGH RATTAN

Yayıncı:

TUBITAK

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 2

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

İngilizce

Document ID (Org No.):

2000811990

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

DOI:

https://doi.org/10.3906/biy-1407-67

Cynarin

artichoke

skin fibroblasts

bone marrow

mesenchymal stem cells

HeLa

stress response

Sayı

Makale detayları

Yazarlar: CEREN GEZER | SEVİNÇ YÜCECAN | SURESH INDER SINGH RATTAN

Yayıncı: TUBITAK

Ana Başlıklar: Yaşam bilimleri

Dergi: Turkish Journal of Biology 2015 Vol.39 Issue 2

Belge Tipi: Makale

Yayın Tarihi: 8/2015

Belge Dili: İngilizce

Document ID (Org No.): 2000811990

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

DOI: https://doi.org/10.3906/biy-1407-67

Sayı

Yazarlar:

CEREN GEZER | SEVİNÇ YÜCECAN | SURESH INDER SINGH RATTAN

Yayıncı:

TUBITAK

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 2

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

İngilizce

Document ID (Org No.):

2000811990

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

DOI:

https://doi.org/10.3906/biy-1407-67