Effects of Plant-derived Smoke, Karrikin, and Salinity Stress on Prunus armeniaca cv. Şalak seeds and seedlings: A Morphological, Biochemical, and Molecular Approach

Yasemin Kemeç Hürkan; Cüneyt Akı

Makale detayları

Effects of Plant-derived Smoke, Karrikin, and Salinity Stress on Prunus armeniaca cv. Şalak seeds and seedlings: A Morphological, Biochemical, and Molecular Approach

Yazarlar:

Yasemin Kemeç Hürkan | Cüneyt Akı

Yayıncı:

Ankara University

Ana Başlıklar:

Agricultural Engineering (Other)

Dergi:

Journal of Agricultural Sciences Vol.30 Issue 2, pp.273-283

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001313940

pISSN:

1300-7580

Anahtar kelimeler:

Özet:

 The effects of plant-derived smoke on seed germination and plant growth, depending on concentration and time, are widely known. However, there are very few studies demonstrating that it provides tolerance to abiotic stresses. This study comprehensively compares the effects of SW and KAR1 on seed germination and morphological, biochemical, and molecular changes observable in the examined seeds. Moreover, the study shows that it regulates the expression of some genes encoding antioxidant enzymes in apricot seedlings Prunus armeniaca L. exposed to salinity stress 100 mM NaCl. The highest germination rate was 1:1000 DS with 60 and 1 μM KAR1 with 72. In terms of shoot development, root and stem length, 1:100 concentration in the DS group and 1 μM concentration in the KAR1 group gave the best results. The shoot development rates were 95.83 and 87.50 in the DS and KAR1 groups, respectively. While the root length was 137.68 and 141.92 mm in the DS and KAR1 groups, respectively, the stem length was 103.78 and 102.67 mm, respectively. The data revealed that SW 1:1000 vv and KAR1 1μM increased the expression levels of catalase CAT, superoxide dismutase SOD, and glutathione peroxidase GPX genes in the samples taken from the apricot seedlings treated with salt at hours 3, 6 and 9. This increase varies in SW and KAR1 depending on time. When the biochemical results were examined, it was seen that the application of SW and KAR1 to the seedlings under salinity stress led to a significant decrease in the thiobarbituric acid reactive substances TBARS content. We can assert that SW is more effective than KAR1 on TBARS content. Morphological, molecular, and biochemical results revealed enhanced germination, growth, gene expression, and TBARS content in apricot seeds and seedlings exposed to SW and KAR1. This data may be applicable to more comprehensive trials.

 The effects of plant-derived smoke on seed germination and plant growth, depending on concentration and time, are widely known. However, there are very few studies demonstrating that it provides tolerance to abiotic stresses. This study comprehensively compares the effects of SW and KAR1 on seed germination and morphological, biochemical, and molecular changes observable in the examined seeds. Moreover, the study shows that it regulates the expression of some genes encoding antioxidant enzymes in apricot seedlings Prunus armeniaca L. exposed to salinity stress 100 mM NaCl. The highest germination rate was 1:1000 DS with 60 and 1 μM KAR1 with 72. In terms of shoot development, root and stem length, 1:100 concentration in the DS group and 1 μM concentration in the KAR1 group gave the best results. The shoot development rates were 95.83 and 87.50 in the DS and KAR1 groups, respectively. While the root length was 137.68 and 141.92 mm in the DS and KAR1 groups, respectively, the stem length was 103.78 and 102.67 mm, respectively. The data revealed that SW 1:1000 vv and KAR1 1μM increased the expression levels of catalase CAT, superoxide dismutase SOD, and glutathione peroxidase GPX genes in the samples taken from the apricot seedlings treated with salt at hours 3, 6 and 9. This increase varies in SW and KAR1 depending on time. When the biochemical results were examined, it was seen that the application of SW and KAR1 to the seedlings under salinity stress led to a significant decrease in the thiobarbituric acid reactive substances TBARS content. We can assert that SW is more effective than KAR1 on TBARS content. Morphological, molecular, and biochemical results revealed enhanced germination, growth, gene expression, and TBARS content in apricot seeds and seedlings exposed to SW and KAR1. This data may be applicable to more comprehensive trials.

 The effects of plant-derived smoke on seed germination and plant growth, depending on concentration and time, are widely known. However, there are very few studies demonstrating that it provides tolerance to abiotic stresses. This study comprehensively compares the effects of SW and KAR1 on seed germination and morphological, biochemical, and molecular changes observable in the examined seeds. Moreover, the study shows that it regulates the expression of some genes encoding antioxidant enzymes in apricot seedlings Prunus armeniaca L. exposed to salinity stress 100 mM NaCl. The highest germination rate was 1:1000 DS with 60 and 1 μM KAR1 with 72. In terms of shoot development, root and stem length, 1:100 concentration in the DS group and 1 μM concentration in the KAR1 group gave the best results. The shoot development rates were 95.83 and 87.50 in the DS and KAR1 groups, respectively. While the root length was 137.68 and 141.92 mm in the DS and KAR1 groups, respectively, the stem length was 103.78 and 102.67 mm, respectively. The data revealed that SW 1:1000 vv and KAR1 1μM increased the expression levels of catalase CAT, superoxide dismutase SOD, and glutathione peroxidase GPX genes in the samples taken from the apricot seedlings treated with salt at hours 3, 6 and 9. This increase varies in SW and KAR1 depending on time. When the biochemical results were examined, it was seen that the application of SW and KAR1 to the seedlings under salinity stress led to a significant decrease in the thiobarbituric acid reactive substances TBARS content. We can assert that SW is more effective than KAR1 on TBARS content. Morphological, molecular, and biochemical results revealed enhanced germination, growth, gene expression, and TBARS content in apricot seeds and seedlings exposed to SW and KAR1. This data may be applicable to more comprehensive trials.

 The effects of plant-derived smoke on seed germination and plant growth, depending on concentration and time, are widely known. However, there are very few studies demonstrating that it provides tolerance to abiotic stresses. This study comprehensively compares the effects of SW and KAR1 on seed germination and morphological, biochemical, and molecular changes observable in the examined seeds. Moreover, the study shows that it regulates the expression of some genes encoding antioxidant enzymes in apricot seedlings Prunus armeniaca L. exposed to salinity stress 100 mM NaCl. The highest germination rate was 1:1000 DS with 60 and 1 μM KAR1 with 72. In terms of shoot development, root and stem length, 1:100 concentration in the DS group and 1 μM concentration in the KAR1 group gave the best results. The shoot development rates were 95.83 and 87.50 in the DS and KAR1 groups, respectively. While the root length was 137.68 and 141.92 mm in the DS and KAR1 groups, respectively, the stem length was 103.78 and 102.67 mm, respectively. The data revealed that SW 1:1000 vv and KAR1 1μM increased the expression levels of catalase CAT, superoxide dismutase SOD, and glutathione peroxidase GPX genes in the samples taken from the apricot seedlings treated with salt at hours 3, 6 and 9. This increase varies in SW and KAR1 depending on time. When the biochemical results were examined, it was seen that the application of SW and KAR1 to the seedlings under salinity stress led to a significant decrease in the thiobarbituric acid reactive substances TBARS content. We can assert that SW is more effective than KAR1 on TBARS content. Morphological, molecular, and biochemical results revealed enhanced germination, growth, gene expression, and TBARS content in apricot seeds and seedlings exposed to SW and KAR1. This data may be applicable to more comprehensive trials.

Makale detayları

Effects of Plant-derived Smoke, Karrikin, and Salinity Stress on Prunus armeniaca cv. Şalak seeds and seedlings: A Morphological, Biochemical, and Molecular Approach

Yazarlar:

Yasemin Kemeç Hürkan | Cüneyt Akı

Yayıncı:

Ankara University

Ana Başlıklar:

Agricultural Engineering (Other)

Dergi:

Journal of Agricultural Sciences Vol.30 Issue 2, pp.273-283

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001313940

pISSN:

1300-7580

Antioxidant enzyme

Smoke water

TBARS

Salt stress

Karrikin

Gene expression

Sayı

Makale detayları

Effects of Plant-derived Smoke, Karrikin, and Salinity Stress on Prunus armeniaca cv. Şalak seeds and seedlings: A Morphological, Biochemical, and Molecular Approach

Yazarlar: Yasemin Kemeç Hürkan | Cüneyt Akı

Yayıncı: Ankara University

Ana Başlıklar: Agricultural Engineering (Other)

Dergi: Journal of Agricultural Sciences Vol.30 Issue 2, pp.273-283

Belge Tipi: Makale

Yayın Tarihi: 3/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001313940

pISSN: 1300-7580

Antioxidant enzyme

Smoke water

TBARS

Salt stress

Karrikin

Gene expression

Sayı

Yazarlar:

Yasemin Kemeç Hürkan | Cüneyt Akı

Yayıncı:

Ankara University

Ana Başlıklar:

Agricultural Engineering (Other)

Dergi:

Journal of Agricultural Sciences Vol.30 Issue 2, pp.273-283

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001313940

pISSN:

1300-7580