Screening of antimicrobial and antiinflammatory activities of three lichenized fungal extracts collected from Northwest Anatolia (Türkiye)

Mehmet Gökhan Halıcı

Makale detayları

Screening of antimicrobial and antiinflammatory activities of three lichenized fungal extracts collected from Northwest Anatolia (Türkiye)

Yazarlar:

Mehmet Gökhan Halıcı

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Journal of Research in Pharmacy Vol.28 Issue 4, pp.1166-1173

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347032

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 In this study, we investigated the anti-microbial and anti-inflammatory activities of the cosmopolite macrolichens Usnea articulata L. Hoffm., Umbilicaria crustulosa Ach. Lamy and Bryoria fuscescens Gyeln. Brodo  D.Hawksw hydroalcoholic extracts to contribute the potential pharmacological uses of lichens. In vitro antimicrobial activities of ethanol extracts against Gram-negative bacteria Escherichia coli, Gram-positive bacteria Staphylococcus aureus, and the yeast Candida albicans were presented using the Broth microdilution method. The most effective lichen extract against gram-positive bacteria S. aureus was U. articulata ethanol extract with a MIC value of 0.125 mgml. U. articulata and B. fuscences extracts have similar anti-fungal activities despite having MIC values of 0.5 mgml. The anti- inflammatory effects of the extracts on LipopolysaccharideInterferon-gamma LPSIF-γ induced macrophage-like cellular systems BV-2 microglia and RAW 264,7 macrophages were evaluated by measuring P38 mitogen-activated protein kinase phosphorylation P38MAPK, cyclooxygenase 2 COX-2 and inducible nitric oxide synthase 2 NOS2 mRNA and protein expression. Especially, Usnea and Umbilicaria extracts also attenuated the LPSIF-γ induced increase in P38MAPK phosphorylation, COX-2, and NOS2 expression in both macrophage-like cells without any cytotoxicity. According to the results of our study, we suggest that the anti-inflammatory mechanism of lichen extracts might result from the inhibition of P38MAPK phosphorylation through a reduction in COX-2 and NOS2 expressions.

 In this study, we investigated the anti-microbial and anti-inflammatory activities of the cosmopolite macrolichens Usnea articulata L. Hoffm., Umbilicaria crustulosa Ach. Lamy and Bryoria fuscescens Gyeln. Brodo  D.Hawksw hydroalcoholic extracts to contribute the potential pharmacological uses of lichens. In vitro antimicrobial activities of ethanol extracts against Gram-negative bacteria Escherichia coli, Gram-positive bacteria Staphylococcus aureus, and the yeast Candida albicans were presented using the Broth microdilution method. The most effective lichen extract against gram-positive bacteria S. aureus was U. articulata ethanol extract with a MIC value of 0.125 mgml. U. articulata and B. fuscences extracts have similar anti-fungal activities despite having MIC values of 0.5 mgml. The anti- inflammatory effects of the extracts on LipopolysaccharideInterferon-gamma LPSIF-γ induced macrophage-like cellular systems BV-2 microglia and RAW 264,7 macrophages were evaluated by measuring P38 mitogen-activated protein kinase phosphorylation P38MAPK, cyclooxygenase 2 COX-2 and inducible nitric oxide synthase 2 NOS2 mRNA and protein expression. Especially, Usnea and Umbilicaria extracts also attenuated the LPSIF-γ induced increase in P38MAPK phosphorylation, COX-2, and NOS2 expression in both macrophage-like cells without any cytotoxicity. According to the results of our study, we suggest that the anti-inflammatory mechanism of lichen extracts might result from the inhibition of P38MAPK phosphorylation through a reduction in COX-2 and NOS2 expressions.

 In this study, we investigated the anti-microbial and anti-inflammatory activities of the cosmopolite macrolichens Usnea articulata L. Hoffm., Umbilicaria crustulosa Ach. Lamy and Bryoria fuscescens Gyeln. Brodo  D.Hawksw hydroalcoholic extracts to contribute the potential pharmacological uses of lichens. In vitro antimicrobial activities of ethanol extracts against Gram-negative bacteria Escherichia coli, Gram-positive bacteria Staphylococcus aureus, and the yeast Candida albicans were presented using the Broth microdilution method. The most effective lichen extract against gram-positive bacteria S. aureus was U. articulata ethanol extract with a MIC value of 0.125 mgml. U. articulata and B. fuscences extracts have similar anti-fungal activities despite having MIC values of 0.5 mgml. The anti- inflammatory effects of the extracts on LipopolysaccharideInterferon-gamma LPSIF-γ induced macrophage-like cellular systems BV-2 microglia and RAW 264,7 macrophages were evaluated by measuring P38 mitogen-activated protein kinase phosphorylation P38MAPK, cyclooxygenase 2 COX-2 and inducible nitric oxide synthase 2 NOS2 mRNA and protein expression. Especially, Usnea and Umbilicaria extracts also attenuated the LPSIF-γ induced increase in P38MAPK phosphorylation, COX-2, and NOS2 expression in both macrophage-like cells without any cytotoxicity. According to the results of our study, we suggest that the anti-inflammatory mechanism of lichen extracts might result from the inhibition of P38MAPK phosphorylation through a reduction in COX-2 and NOS2 expressions.

 In this study, we investigated the anti-microbial and anti-inflammatory activities of the cosmopolite macrolichens Usnea articulata L. Hoffm., Umbilicaria crustulosa Ach. Lamy and Bryoria fuscescens Gyeln. Brodo  D.Hawksw hydroalcoholic extracts to contribute the potential pharmacological uses of lichens. In vitro antimicrobial activities of ethanol extracts against Gram-negative bacteria Escherichia coli, Gram-positive bacteria Staphylococcus aureus, and the yeast Candida albicans were presented using the Broth microdilution method. The most effective lichen extract against gram-positive bacteria S. aureus was U. articulata ethanol extract with a MIC value of 0.125 mgml. U. articulata and B. fuscences extracts have similar anti-fungal activities despite having MIC values of 0.5 mgml. The anti- inflammatory effects of the extracts on LipopolysaccharideInterferon-gamma LPSIF-γ induced macrophage-like cellular systems BV-2 microglia and RAW 264,7 macrophages were evaluated by measuring P38 mitogen-activated protein kinase phosphorylation P38MAPK, cyclooxygenase 2 COX-2 and inducible nitric oxide synthase 2 NOS2 mRNA and protein expression. Especially, Usnea and Umbilicaria extracts also attenuated the LPSIF-γ induced increase in P38MAPK phosphorylation, COX-2, and NOS2 expression in both macrophage-like cells without any cytotoxicity. According to the results of our study, we suggest that the anti-inflammatory mechanism of lichen extracts might result from the inhibition of P38MAPK phosphorylation through a reduction in COX-2 and NOS2 expressions.

Makale detayları

Screening of antimicrobial and antiinflammatory activities of three lichenized fungal extracts collected from Northwest Anatolia (Türkiye)

Yazarlar:

Mehmet Gökhan Halıcı

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Journal of Research in Pharmacy Vol.28 Issue 4, pp.1166-1173

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347032

pISSN:

2630-6344

Inflammation

Lichens

Microglia

Lipopolysaccharide

Interferon-gamma

Sayı

Makale detayları

Screening of antimicrobial and antiinflammatory activities of three lichenized fungal extracts collected from Northwest Anatolia (Türkiye)

Yazarlar: Mehmet Gökhan Halıcı

Yayıncı: Marmara University

Ana Başlıklar: Pharmacology and Pharmaceutical Sciences (Other)

Dergi: Journal of Research in Pharmacy Vol.28 Issue 4, pp.1166-1173

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001347032

pISSN: 2630-6344

Inflammation

Lichens

Microglia

Lipopolysaccharide

Interferon-gamma

Sayı

Yazarlar:

Mehmet Gökhan Halıcı

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Journal of Research in Pharmacy Vol.28 Issue 4, pp.1166-1173

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347032

pISSN:

2630-6344