Effect of Tryptanthrin Compound on Lipop olysa cchar ide-I nduce d Inflammation Model in L929 Cell Line

Zerrin Kutlu

Makale detayları

Effect of Tryptanthrin Compound on Lipop olysa cchar ide-I nduce d Inflammation Model in L929 Cell Line

Yazarlar:

Zerrin Kutlu

Yayıncı:

Ataturk University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Pharmata Vol.3 Issue 3, pp.54-58

Belge Tipi:

Makale

Yayın Tarihi:

7/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001294326

pISSN:

2980-1966

Anahtar kelimeler:

Özet:

 Objective: Our aim is to evaluate the effects of the tryptanthrin compound, with antimicrobial and anti-inflammatory effects, on the lipopolysaccharide inflammation model created in the L929 cell line. Methods: L929 cell lines were placed in a 5 CO2 incubator at 37C, using standard cell culture procedures, and lipopolysaccharide was applied with Prn pure substance with a final concentration of 2 μL and 1 μL after 1 hour. Following the lipopolysaccharide application, the 3- 4,5- dimethylthiazol- 2-yl -2,5-diphenyl tetrazolium bromide protocol, a colorimetric method, was applied to determine cell viability at the end of the required 24th, 48th, and 72nd hours incubation times. Results: IC50 values were calculated as 0.3 μgmL for tryptanthrin and 1 μgmL for lipopolysaccharide . Application of lipopolysaccharide to L929 cell lines caused a significant decrease in cellular index depending on time. Also, in the tryptanthrin  lipopolysaccharide groups, it was found that the decreased cell index significantly increased even closer to the control compared to the lipopolysaccharide applied group. It was found that the inflammation and cell damage caused by lipopolysaccharide applied to the L929 cell line improved after tryptanthrin application. Conclusion: When we look at the results of our study as a whole, it was determined that the viability rates of the cells were above 85 as a result of the tryptanthrin application and it was significantly above the 70 viable cell ratio recommended by ISO 10993-5: 2009 Dın en ISO 2009 for cytotoxicity.

 Objective: Our aim is to evaluate the effects of the tryptanthrin compound, with antimicrobial and anti-inflammatory effects, on the lipopolysaccharide inflammation model created in the L929 cell line. Methods: L929 cell lines were placed in a 5 CO2 incubator at 37C, using standard cell culture procedures, and lipopolysaccharide was applied with Prn pure substance with a final concentration of 2 μL and 1 μL after 1 hour. Following the lipopolysaccharide application, the 3- 4,5- dimethylthiazol- 2-yl -2,5-diphenyl tetrazolium bromide protocol, a colorimetric method, was applied to determine cell viability at the end of the required 24th, 48th, and 72nd hours incubation times. Results: IC50 values were calculated as 0.3 μgmL for tryptanthrin and 1 μgmL for lipopolysaccharide . Application of lipopolysaccharide to L929 cell lines caused a significant decrease in cellular index depending on time. Also, in the tryptanthrin  lipopolysaccharide groups, it was found that the decreased cell index significantly increased even closer to the control compared to the lipopolysaccharide applied group. It was found that the inflammation and cell damage caused by lipopolysaccharide applied to the L929 cell line improved after tryptanthrin application. Conclusion: When we look at the results of our study as a whole, it was determined that the viability rates of the cells were above 85 as a result of the tryptanthrin application and it was significantly above the 70 viable cell ratio recommended by ISO 10993-5: 2009 Dın en ISO 2009 for cytotoxicity.

 Objective: Our aim is to evaluate the effects of the tryptanthrin compound, with antimicrobial and anti-inflammatory effects, on the lipopolysaccharide inflammation model created in the L929 cell line. Methods: L929 cell lines were placed in a 5 CO2 incubator at 37C, using standard cell culture procedures, and lipopolysaccharide was applied with Prn pure substance with a final concentration of 2 μL and 1 μL after 1 hour. Following the lipopolysaccharide application, the 3- 4,5- dimethylthiazol- 2-yl -2,5-diphenyl tetrazolium bromide protocol, a colorimetric method, was applied to determine cell viability at the end of the required 24th, 48th, and 72nd hours incubation times. Results: IC50 values were calculated as 0.3 μgmL for tryptanthrin and 1 μgmL for lipopolysaccharide . Application of lipopolysaccharide to L929 cell lines caused a significant decrease in cellular index depending on time. Also, in the tryptanthrin  lipopolysaccharide groups, it was found that the decreased cell index significantly increased even closer to the control compared to the lipopolysaccharide applied group. It was found that the inflammation and cell damage caused by lipopolysaccharide applied to the L929 cell line improved after tryptanthrin application. Conclusion: When we look at the results of our study as a whole, it was determined that the viability rates of the cells were above 85 as a result of the tryptanthrin application and it was significantly above the 70 viable cell ratio recommended by ISO 10993-5: 2009 Dın en ISO 2009 for cytotoxicity.

 Objective: Our aim is to evaluate the effects of the tryptanthrin compound, with antimicrobial and anti-inflammatory effects, on the lipopolysaccharide inflammation model created in the L929 cell line. Methods: L929 cell lines were placed in a 5 CO2 incubator at 37C, using standard cell culture procedures, and lipopolysaccharide was applied with Prn pure substance with a final concentration of 2 μL and 1 μL after 1 hour. Following the lipopolysaccharide application, the 3- 4,5- dimethylthiazol- 2-yl -2,5-diphenyl tetrazolium bromide protocol, a colorimetric method, was applied to determine cell viability at the end of the required 24th, 48th, and 72nd hours incubation times. Results: IC50 values were calculated as 0.3 μgmL for tryptanthrin and 1 μgmL for lipopolysaccharide . Application of lipopolysaccharide to L929 cell lines caused a significant decrease in cellular index depending on time. Also, in the tryptanthrin  lipopolysaccharide groups, it was found that the decreased cell index significantly increased even closer to the control compared to the lipopolysaccharide applied group. It was found that the inflammation and cell damage caused by lipopolysaccharide applied to the L929 cell line improved after tryptanthrin application. Conclusion: When we look at the results of our study as a whole, it was determined that the viability rates of the cells were above 85 as a result of the tryptanthrin application and it was significantly above the 70 viable cell ratio recommended by ISO 10993-5: 2009 Dın en ISO 2009 for cytotoxicity.

Makale detayları

Effect of Tryptanthrin Compound on Lipop olysa cchar ide-I nduce d Inflammation Model in L929 Cell Line

Yazarlar:

Zerrin Kutlu

Yayıncı:

Ataturk University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Pharmata Vol.3 Issue 3, pp.54-58

Belge Tipi:

Makale

Yayın Tarihi:

7/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001294326

pISSN:

2980-1966

Inflammation

L929

LPS

MTT

Tryptanthrin

Sayı

Makale detayları

Effect of Tryptanthrin Compound on Lipop olysa cchar ide-I nduce d Inflammation Model in L929 Cell Line

Yazarlar: Zerrin Kutlu

Yayıncı: Ataturk University

Ana Başlıklar: Pharmacology and Pharmaceutical Sciences (Other)

Dergi: Pharmata Vol.3 Issue 3, pp.54-58

Belge Tipi: Makale

Yayın Tarihi: 7/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001294326

pISSN: 2980-1966

Inflammation

L929

LPS

MTT

Tryptanthrin

Sayı

Yazarlar:

Zerrin Kutlu

Yayıncı:

Ataturk University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Pharmata Vol.3 Issue 3, pp.54-58

Belge Tipi:

Makale

Yayın Tarihi:

7/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001294326

pISSN:

2980-1966