Green Synthesis, Characterization and Antibacterial Activities of Silk Sericin Capped Zinc Oxide Nanoparticles

Zehra Gün Gök

Makale detayları

Green Synthesis, Characterization and Antibacterial Activities of Silk Sericin Capped Zinc Oxide Nanoparticles

Yazarlar:

Zehra Gün Gök

Yayıncı:

Marmara University

Ana Başlıklar:

Kompozit ve Hibrit Malzemeler | Malzeme Karekterizasyonu | Malzeme Üretim Teknolojileri

Dergi:

International Journal of Advances in Engineering and Pure Sciences Vol.36 Issue 2, pp.150-159

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001319790

pISSN:

2636-8277

Anahtar kelimeler:

Özet:

 In recent years, interest in metal-based antibacterial materials has increased due to microorganisms gaining resistance to antibiotics. Silk sericin obtained from Bombyx mori cocoon has found use in many different areas thanks to its biocompatibility, hydrophilic character and biodegradability. Zinc oxide nanoparticles ZnONPs obtained in various zinc salts exhibit broad-spectrum antibacterial properties. In this study, to be produce metal based antibacterial materials, synthesis of silk sericin-coated ZnONPs SS-ZnONPs in a green and scalable method was investigated by using silk sericin protein as both reducing and capping agent to obtain ZnONPs. For producing SS-ZnONPs, 2 silk sericin solution was mixed with ZnNO32 solution and the blend solution was heated at 100 C for a certain period of time. Observing surface plasmon resonance SPR peak specific at 380 nm in the UV-vis spectrum of SS-ZnONPs represented the formation of ZnONPs. Then, the chemical, morphological, crystalline, thermal, and antibacterial properties of the synthesized SS-ZnONPs were examined. Characteristic peak of the Zn-O band was found in fourier transform infrared spectroscopy FTIR analysis of SS-ZnONPs. According to scanning electron microscopy SEM analyses, ZnONPs had morphology similar to cubichexagonal shape, showed a uniform structure, and did not represent any agglomerations. In energy dispersive spectroscopy EDS analyses of SS-ZnONPs, peaks belonging to carbon, nitrogen, oxygen, sulphur, and zinc elements were observed. The formation of Zn peak indicated that the zinc ions were transformed into ZnONPs. In addition, characteristic peaks of zinc were seen in the X-ray diffractometer XRD result of SS-ZnONPs. Thermogravimetric analysis TGA showed that the thermal stability and remaining amount of SS-ZnONPs was higher compared to pure silk sericin powder due to the formation of ZnONPs. Lastly, agar well diffusion test was carried out with Staphylococcus aureus ATCC 6538 and Escherichia coli ATCC 25922 bacteria and SS-ZnONPs showed antibacterial action against S. aureus. It has been observed that the obtained SS-ZnONPs can be used as antibacterial agents. However, it was also understood that the ZnONPs concentration in this study was low for high antibacterial activity.

 In recent years, interest in metal-based antibacterial materials has increased due to microorganisms gaining resistance to antibiotics. Silk sericin obtained from Bombyx mori cocoon has found use in many different areas thanks to its biocompatibility, hydrophilic character and biodegradability. Zinc oxide nanoparticles ZnONPs obtained in various zinc salts exhibit broad-spectrum antibacterial properties. In this study, to be produce metal based antibacterial materials, synthesis of silk sericin-coated ZnONPs SS-ZnONPs in a green and scalable method was investigated by using silk sericin protein as both reducing and capping agent to obtain ZnONPs. For producing SS-ZnONPs, 2 silk sericin solution was mixed with ZnNO32 solution and the blend solution was heated at 100 C for a certain period of time. Observing surface plasmon resonance SPR peak specific at 380 nm in the UV-vis spectrum of SS-ZnONPs represented the formation of ZnONPs. Then, the chemical, morphological, crystalline, thermal, and antibacterial properties of the synthesized SS-ZnONPs were examined. Characteristic peak of the Zn-O band was found in fourier transform infrared spectroscopy FTIR analysis of SS-ZnONPs. According to scanning electron microscopy SEM analyses, ZnONPs had morphology similar to cubichexagonal shape, showed a uniform structure, and did not represent any agglomerations. In energy dispersive spectroscopy EDS analyses of SS-ZnONPs, peaks belonging to carbon, nitrogen, oxygen, sulphur, and zinc elements were observed. The formation of Zn peak indicated that the zinc ions were transformed into ZnONPs. In addition, characteristic peaks of zinc were seen in the X-ray diffractometer XRD result of SS-ZnONPs. Thermogravimetric analysis TGA showed that the thermal stability and remaining amount of SS-ZnONPs was higher compared to pure silk sericin powder due to the formation of ZnONPs. Lastly, agar well diffusion test was carried out with Staphylococcus aureus ATCC 6538 and Escherichia coli ATCC 25922 bacteria and SS-ZnONPs showed antibacterial action against S. aureus. It has been observed that the obtained SS-ZnONPs can be used as antibacterial agents. However, it was also understood that the ZnONPs concentration in this study was low for high antibacterial activity.

 In recent years, interest in metal-based antibacterial materials has increased due to microorganisms gaining resistance to antibiotics. Silk sericin obtained from Bombyx mori cocoon has found use in many different areas thanks to its biocompatibility, hydrophilic character and biodegradability. Zinc oxide nanoparticles ZnONPs obtained in various zinc salts exhibit broad-spectrum antibacterial properties. In this study, to be produce metal based antibacterial materials, synthesis of silk sericin-coated ZnONPs SS-ZnONPs in a green and scalable method was investigated by using silk sericin protein as both reducing and capping agent to obtain ZnONPs. For producing SS-ZnONPs, 2 silk sericin solution was mixed with ZnNO32 solution and the blend solution was heated at 100 C for a certain period of time. Observing surface plasmon resonance SPR peak specific at 380 nm in the UV-vis spectrum of SS-ZnONPs represented the formation of ZnONPs. Then, the chemical, morphological, crystalline, thermal, and antibacterial properties of the synthesized SS-ZnONPs were examined. Characteristic peak of the Zn-O band was found in fourier transform infrared spectroscopy FTIR analysis of SS-ZnONPs. According to scanning electron microscopy SEM analyses, ZnONPs had morphology similar to cubichexagonal shape, showed a uniform structure, and did not represent any agglomerations. In energy dispersive spectroscopy EDS analyses of SS-ZnONPs, peaks belonging to carbon, nitrogen, oxygen, sulphur, and zinc elements were observed. The formation of Zn peak indicated that the zinc ions were transformed into ZnONPs. In addition, characteristic peaks of zinc were seen in the X-ray diffractometer XRD result of SS-ZnONPs. Thermogravimetric analysis TGA showed that the thermal stability and remaining amount of SS-ZnONPs was higher compared to pure silk sericin powder due to the formation of ZnONPs. Lastly, agar well diffusion test was carried out with Staphylococcus aureus ATCC 6538 and Escherichia coli ATCC 25922 bacteria and SS-ZnONPs showed antibacterial action against S. aureus. It has been observed that the obtained SS-ZnONPs can be used as antibacterial agents. However, it was also understood that the ZnONPs concentration in this study was low for high antibacterial activity.

 Son yıllarda mikroorganizmaların antibiyotiklere karşı direnç kazanması nedeniyle metal bazlı antibakteriyel malzemelere olan ilgi artmıştır. Bombyx mori kozasından elde edilen ipek serisin, biyouyumluluğu, hidrofilik karakteri ve biyolojik olarak parçalanabilirliği sayesinde birçok farklı alanda kullanım alanı bulmuştur. Çeşitli çinko tuzlarından elde edilen çinko oksit nanopartikülleri ZnONPler, geniş spektrumlu antibakteriyel özellikler sergilemektedir. Bu çalışmada, metal bazlı antibakteriyel malzeme üretmek amacıyla, ZnONP elde etmek için ipek serisin proteini hem indirgeyici hem de kaplayıcı madde olarak kullanılarak yeşil ve ölçeklenebilir bir yöntemle ipek serisin kaplı ZnONPlerin SS-ZnONPler sentezi araştırılmıştır. SS-ZnONPlerin üretimi için 2lik ipek serisin çözeltisi ZnNO32 çözeltisi ile karıştırılmış ve karışım çözeltisi 100 Cde belirli bir süre ısıtılmıştır. SS-ZnONPlerin UV-vis spektrumunda 380 nmde spesifik yüzey plazmon rezonansı SPR zirvesinin gözlemlenmesi, ZnONPlerin oluşumunu göstermektedir. Daha sonra sentezlenen SS-ZnONPlerin kimyasal, morfolojik, kristal, termal ve antibakteriyel özellikleri incelenmiştir. Zn-O bandının karakteristik zirvesi, SS-ZnONPlerin fourier transform kızılötesi spektroskopisi FTIR analizinde görülmüştür. Taramalı elektron mikroskobu SEM analizlerine göre ZnONPlerin kübikaltıgen şekle benzer bir morfolojiye sahip olduğu, tekdüze bir yapı gösterdiği ve herhangi bir topaklanma göstermediği görülmüştür. SS-ZnONPlerin enerji dağılımlı spektroskopi EDS analizlerinde karbon, azot, oksijen, kükürt ve çinko elementlerine ait pikler görülmüştür. Zn zirvesinin oluşması çinko iyonlarının ZnONPlere dönüştüğünü göstermektedir. Ek olarak, SS-ZnONPlerin X-ışını difraktometresi XRD sonucunda karakteristik çinko zirveleri vardır. Termogravimetrik analiz TGA, ZnONP oluşumu nedeniyle SS-ZnONPlerin termal stabilitesinin ve kalan miktarının saf ipek serisin tozuna kıyasla daha yüksek olduğunu göstermektedir. Son olarak Staphylococcus aureus ATCC 6538 ve Escherichia coli ATCC 25922 bakterileri ile agar kuyucuk difüzyon testi yapılmış ve SS-ZnONPler S. aureusa karşı antibakteriyel etki göstermiştir. Elde edilen SS-ZnONPlerin antibakteriyel ajan olarak kullanılabileceği görülmüştür. Ancak bu çalışmada ZnONP konsantrasyonunun yüksek antibakteriyel aktivite nedeniyle düşük olduğu da anlaşılmıştır.

Makale detayları

Green Synthesis, Characterization and Antibacterial Activities of Silk Sericin Capped Zinc Oxide Nanoparticles

Yazarlar:

Zehra Gün Gök

Yayıncı:

Marmara University

Ana Başlıklar:

Kompozit ve Hibrit Malzemeler | Malzeme Karekterizasyonu | Malzeme Üretim Teknolojileri

Dergi:

International Journal of Advances in Engineering and Pure Sciences Vol.36 Issue 2, pp.150-159

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001319790

pISSN:

2636-8277

Green synthesis

Silk sericin

Zinc oxide nanoparticles

Antibacterial materialsYeşil sentez

İpek serisin

Çinko oksit nanopartikülleri

Antibakteriyel malzemeler

Sayı

Makale detayları

Yazarlar: Zehra Gün Gök

Yayıncı: Marmara University

Ana Başlıklar: Kompozit ve Hibrit Malzemeler | Malzeme Karekterizasyonu | Malzeme Üretim Teknolojileri

Dergi: International Journal of Advances in Engineering and Pure Sciences Vol.36 Issue 2, pp.150-159

Belge Tipi: Makale

Yayın Tarihi: 1/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001319790

pISSN: 2636-8277

Sayı

Yazarlar:

Zehra Gün Gök

Yayıncı:

Marmara University

Ana Başlıklar:

Kompozit ve Hibrit Malzemeler | Malzeme Karekterizasyonu | Malzeme Üretim Teknolojileri

Dergi:

International Journal of Advances in Engineering and Pure Sciences Vol.36 Issue 2, pp.150-159

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001319790

pISSN:

2636-8277