Tumor necrosis factor-alpha induced caspase-3 activation-related iNOS gene expression in ADP-activated platelets

ÖZGE ÇEVİK; ZELAL ADIGÜZEL; AHMET TARIK BAYKAL; AZİZE ŞENER

Makale detayları

Tumor necrosis factor-alpha induced caspase-3 activation-related iNOS gene expression in ADP-activated platelets

Yazarlar:

ÖZGE ÇEVİK | ZELAL ADIGÜZEL | AHMET TARIK BAYKAL | AZİZE ŞENER

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2017 Vol.41 Issue 1, pp.31-40

Belge Tipi:

Makale

Yayın Tarihi:

1/2017

Belge Dili:

Türkçe

Document ID (Org No.):

2000811454

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Özet:

 

Platelets are sensitive cells and are easily activated by different stimulants in the circulation system. It is known that tumor necrosis factor-alpha (TNF-α) is a proinflammatory cytokine and plays a role in inflammation. The role of TNF-α in the apoptotic process in blood platelets is unknown. In order to study the formation of apoptosis in platelets after incubation with TNF-α and/or ADP, several biomarkers were chosen: phosphatidylserine (PS) exposure and P-selectin binding; cGMP, Cyt-c, and Ca2+ levels and NOS activation; and gene and protein expression of caspase-3 and iNOS. Platelets were incubated with 100 pg/mL TNF-α and/or 50 mM iNOS specific inhibitor, such as at 1400 W for 1 h at 37 °C in the presence of 5 µM ADP. According to the results, the levels of PS exposure and P-selectin binding were significantly higher in TNF-α + ADP platelets, which decreased with the addition of 1400 W. A significant induction in cGMP, Cyt-c, and Ca2+ levels was observed in platelets treated with TNF-α + ADP. On the other hand, the upregulation of the apoptotic gene caspase-3 and iNOS expression levels in TNF-α-treated and ADP-activated platelets was significantly reversed with the addition of 1400 W. These data demonstrate that TNF-α promotes iNOS expression through caspase-3 stimulation in human platelets, and its concomitance leads to the triggering of apoptosis-mediated inflammation upon platelet activation.

Makale detayları

Tumor necrosis factor-alpha induced caspase-3 activation-related iNOS gene expression in ADP-activated platelets

Yazarlar: ÖZGE ÇEVİK | ZELAL ADIGÜZEL | AHMET TARIK BAYKAL | AZİZE ŞENER

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2017 Vol.41 Issue 1, pp.31-40

Belge Tipi: Makale

Yayın Tarihi: 1/2017

Belge Dili: Türkçe

Document ID (Org No.): 2000811454

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Sayı

Yazarlar:

ÖZGE ÇEVİK | ZELAL ADIGÜZEL | AHMET TARIK BAYKAL | AZİZE ŞENER

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2017 Vol.41 Issue 1, pp.31-40

Belge Tipi:

Makale

Yayın Tarihi:

1/2017

Belge Dili:

Türkçe

Document ID (Org No.):

2000811454

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152