The in vitro determination of genotoxicity in peripheral lymphocytes of welders exposed to fumes from metal arc welding

Ceylan ŞENER; Halil Erhan EROĞLU

Makale detayları

The in vitro determination of genotoxicity in peripheral lymphocytes of welders exposed to fumes from metal arc welding

Yazarlar:

Ceylan ŞENER | Halil Erhan EROĞLU

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2013 Vol.43 Issue 3, pp.411-416

Belge Tipi:

Makale

Yayın Tarihi:

2/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000753729

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Anahtar kelimeler:

Özet:

 

Gas metal arc welding is a widely used method in many industrial areas. In this study, the peripheral blood lymphocytes of 23 welders and 25 nonexposed subjects were monitored for genotoxicity.   Materials and methods: The blood samples obtained from the subjects were incubated with a culture medium at 37 °C for 72 h. Cytochalasin-B and BrdU were added for micronucleus and replication index. After incubation, the cultures were harvested, stained, and examined.  Results: The micronucleus rate (2.165 ± 1.645) of the welders was quite higher than that of those not exposed (1.352 ± 0.978) (P < 0.01). There was a significant difference between micronucleus rates of smoking and nonsmoking subjects. It was found that as the time of exposure to welding fumes increased, so did the micronucleus rates (2.938 ± 1.723) (P < 0.05). A positive correlation was observed between micronucleus frequency and age. The replication index rate (1.026 ± 0.020) of the welders was higher than that of those not exposed (1.019 ± 0.016) (P = 0.01).  Conclusion: It is advised that welders who work with gas metal arc welding be instructed and that necessary protective measures be taken because of the risk caused by metal arc welding fumes.

 

Gas metal arc welding is a widely used method in many industrial areas. In this study, the peripheral blood lymphocytes of 23 welders and 25 nonexposed subjects were monitored for genotoxicity.   Materials and methods: The blood samples obtained from the subjects were incubated with a culture medium at 37 °C for 72 h. Cytochalasin-B and BrdU were added for micronucleus and replication index. After incubation, the cultures were harvested, stained, and examined.  Results: The micronucleus rate (2.165 ± 1.645) of the welders was quite higher than that of those not exposed (1.352 ± 0.978) (P < 0.01). There was a significant difference between micronucleus rates of smoking and nonsmoking subjects. It was found that as the time of exposure to welding fumes increased, so did the micronucleus rates (2.938 ± 1.723) (P < 0.05). A positive correlation was observed between micronucleus frequency and age. The replication index rate (1.026 ± 0.020) of the welders was higher than that of those not exposed (1.019 ± 0.016) (P = 0.01).  Conclusion: It is advised that welders who work with gas metal arc welding be instructed and that necessary protective measures be taken because of the risk caused by metal arc welding fumes.

Makale detayları

The in vitro determination of genotoxicity in peripheral lymphocytes of welders exposed to fumes from metal arc welding

Yazarlar:

Ceylan ŞENER | Halil Erhan EROĞLU

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2013 Vol.43 Issue 3, pp.411-416

Belge Tipi:

Makale

Yayın Tarihi:

2/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000753729

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Key words: Gas metal arc welding

micronucleus

replication index

welder

Sayı

Makale detayları

The in vitro determination of genotoxicity in peripheral lymphocytes of welders exposed to fumes from metal arc welding

Yazarlar: Ceylan ŞENER | Halil Erhan EROĞLU

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2013 Vol.43 Issue 3, pp.411-416

Belge Tipi: Makale

Yayın Tarihi: 2/2013

Belge Dili: Türkçe

Document ID (Org No.): 2000753729

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Key words: Gas metal arc welding

micronucleus

replication index

welder

Sayı

Yazarlar:

Ceylan ŞENER | Halil Erhan EROĞLU

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2013 Vol.43 Issue 3, pp.411-416

Belge Tipi:

Makale

Yayın Tarihi:

2/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000753729

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144