Antimicrobial susceptibilities of Aeromonas species isolated from medical leeches

Dilek Öztürk; Sibel Yaman; Azra Demirci Özdemir

Makale detayları

Antimicrobial susceptibilities of Aeromonas species isolated from medical leeches

Yazarlar:

Dilek Öztürk | Sibel Yaman | Azra Demirci Özdemir

Yayıncı:

Burdur Mehmet Akif Ersoy University

Ana Başlıklar:

Veterinary Microbiology

Dergi:

Mediterranean Veterinary Journal Vol.10 Issue 1, pp.416-420

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347256

pISSN:

3023-8447

Anahtar kelimeler:

Özet:

 The aim of this study was to determine the antibiotic susceptibility of Aeromonas species isolated from their environment and leeches are grown for use in medical treatment. The water samples and two leeches which 2-10 g weight from a leech hatchery were brought to XXXXX. The samples were cultured on blood agar base added 7 defibrined sheep blood and MacConkey agar at 18-20 hours and 37C. Besides the samples were incubated on two Sabourrauds Dextrose agar supplemented chloramphenicol at 20 C and 37C for 10 -14 days. Two different colonies was detected to be Aeromonas spp. by conventional microbiological methods such as Gram staining, haemolysis, catalase, oxidase, triple tube methods. These colonies were identified to be Aeromonas hydrophila and Aeromonas veronii on species level by Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry, too. According to antimicrobial susceptibiliy tests, while Aeromonas hydrophila and Aeromonas veronii isolates were found resistant to amoxycillin, ampicilin, gentamicin, erytromycin, oxacillin and penicillin, these isolates were susceptible to ceftriaxone, enrofloxacin, florfenicol and trimethoprim-sulphamethaxazole. Because of isolation and identification of Aeromonas hydrophila and Aeromonas veronii from medical leeches and its environmental, and antimicrobial resistances to several antibiotics, leech therapy may be a potential public health problem for humans.

 The aim of this study was to determine the antibiotic susceptibility of Aeromonas species isolated from their environment and leeches are grown for use in medical treatment. The water samples and two leeches which 2-10 g weight from a leech hatchery were brought to XXXXX. The samples were cultured on blood agar base added 7 defibrined sheep blood and MacConkey agar at 18-20 hours and 37C. Besides the samples were incubated on two Sabourrauds Dextrose agar supplemented chloramphenicol at 20 C and 37C for 10 -14 days. Two different colonies was detected to be Aeromonas spp. by conventional microbiological methods such as Gram staining, haemolysis, catalase, oxidase, triple tube methods. These colonies were identified to be Aeromonas hydrophila and Aeromonas veronii on species level by Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry, too. According to antimicrobial susceptibiliy tests, while Aeromonas hydrophila and Aeromonas veronii isolates were found resistant to amoxycillin, ampicilin, gentamicin, erytromycin, oxacillin and penicillin, these isolates were susceptible to ceftriaxone, enrofloxacin, florfenicol and trimethoprim-sulphamethaxazole. Because of isolation and identification of Aeromonas hydrophila and Aeromonas veronii from medical leeches and its environmental, and antimicrobial resistances to several antibiotics, leech therapy may be a potential public health problem for humans.

 The aim of this study was to determine the antibiotic susceptibility of Aeromonas species isolated from their environment and leeches are grown for use in medical treatment. The water samples and two leeches which 2-10 g weight from a leech hatchery were brought to XXXXX. The samples were cultured on blood agar base added 7 defibrined sheep blood and MacConkey agar at 18-20 hours and 37C. Besides the samples were incubated on two Sabourrauds Dextrose agar supplemented chloramphenicol at 20 C and 37C for 10 -14 days. Two different colonies was detected to be Aeromonas spp. by conventional microbiological methods such as Gram staining, haemolysis, catalase, oxidase, triple tube methods. These colonies were identified to be Aeromonas hydrophila and Aeromonas veronii on species level by Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry, too. According to antimicrobial susceptibiliy tests, while Aeromonas hydrophila and Aeromonas veronii isolates were found resistant to amoxycillin, ampicilin, gentamicin, erytromycin, oxacillin and penicillin, these isolates were susceptible to ceftriaxone, enrofloxacin, florfenicol and trimethoprim-sulphamethaxazole. Because of isolation and identification of Aeromonas hydrophila and Aeromonas veronii from medical leeches and its environmental, and antimicrobial resistances to several antibiotics, leech therapy may be a potential public health problem for humans.

 The aim of this study was to determine the antibiotic susceptibility of Aeromonas species isolated from their environment and leeches are grown for use in medical treatment. The water samples and two leeches which 2-10 g weight from a leech hatchery were brought to XXXXX. The samples were cultured on blood agar base added 7 defibrined sheep blood and MacConkey agar at 18-20 hours and 37C. Besides the samples were incubated on two Sabourrauds Dextrose agar supplemented chloramphenicol at 20 C and 37C for 10 -14 days. Two different colonies was detected to be Aeromonas spp. by conventional microbiological methods such as Gram staining, haemolysis, catalase, oxidase, triple tube methods. These colonies were identified to be Aeromonas hydrophila and Aeromonas veronii on species level by Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry, too. According to antimicrobial susceptibiliy tests, while Aeromonas hydrophila and Aeromonas veronii isolates were found resistant to amoxycillin, ampicilin, gentamicin, erytromycin, oxacillin and penicillin, these isolates were susceptible to ceftriaxone, enrofloxacin, florfenicol and trimethoprim-sulphamethaxazole. Because of isolation and identification of Aeromonas hydrophila and Aeromonas veronii from medical leeches and its environmental, and antimicrobial resistances to several antibiotics, leech therapy may be a potential public health problem for humans.

Makale detayları

Antimicrobial susceptibilities of Aeromonas species isolated from medical leeches

Yazarlar:

Dilek Öztürk | Sibel Yaman | Azra Demirci Özdemir

Yayıncı:

Burdur Mehmet Akif Ersoy University

Ana Başlıklar:

Veterinary Microbiology

Dergi:

Mediterranean Veterinary Journal Vol.10 Issue 1, pp.416-420

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347256

pISSN:

3023-8447

Key words: Leeches

Aeromonas hydrophila

A. veronii

antimicrobial resistance

Sayı

Makale detayları

Antimicrobial susceptibilities of Aeromonas species isolated from medical leeches

Yazarlar: Dilek Öztürk | Sibel Yaman | Azra Demirci Özdemir

Yayıncı: Burdur Mehmet Akif Ersoy University

Ana Başlıklar: Veterinary Microbiology

Dergi: Mediterranean Veterinary Journal Vol.10 Issue 1, pp.416-420

Belge Tipi: Makale

Yayın Tarihi: 4/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001347256

pISSN: 3023-8447

Key words: Leeches

Aeromonas hydrophila

A. veronii

antimicrobial resistance

Sayı

Yazarlar:

Dilek Öztürk | Sibel Yaman | Azra Demirci Özdemir

Yayıncı:

Burdur Mehmet Akif Ersoy University

Ana Başlıklar:

Veterinary Microbiology

Dergi:

Mediterranean Veterinary Journal Vol.10 Issue 1, pp.416-420

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347256

pISSN:

3023-8447