Temperature-Dependent Thermotaxis and Its Impact on Motility, Viability, and Concentration in Canine (American Bully) Sperm

Ali Galip Önal

Makale detayları

Temperature-Dependent Thermotaxis and Its Impact on Motility, Viability, and Concentration in Canine (American Bully) Sperm

Yazarlar:

Ali Galip Önal

Yayıncı:

Cukurova University

Ana Başlıklar:

Zootekni | Genetik ve Biyoistatistik

Dergi:

Çukurova Tarım ve Gıda Bilimleri Dergisi Vol.40 Issue 1, pp.140-147

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001353359

pISSN:

2636-7874

Anahtar kelimeler:

Özet:

 Sperm transfer through the reproductive tract is crucial for successful fertilization in most of mammals. After ejaculation, spermatozoa need to go through a complex physiological barrier and environments in the reproductive tract. Also, sperm must respond to many environmental conditions, including temperature. The present study aims to investigate the thermotactic response of sperms collected from American Bully dogs and its impact on motility, viability, membrane integrity, and concentration at different temperatures. Evaluated semen samples, collected from mature dogs incubated at 34C, 38C, and 40C for 60 minutes in a custom-designed thermotaxis model. Some sperm parameters were assessed using computer-assisted sperm analysis iSperm CASA, Nigrosin-Eosin staining, and the Hypo-Osmotic Swelling Test HOST. Results showed that sperm motility and concentration were increased at 34C and 38C, while exposure to 40C resulted in a significant decline in motility, progressive motility, and concentration. Slightly higher curvilinear velocity VCL and straight-line velocity VSL at 38C suggests optimal motility at this temperature. Membrane integrity HOST-positive sperm was significantly compromised at 40C, with a corresponding decrease in viability, as confirmed by Nigrosin-Eosin staining. Sperm direction towards to different temperature for the sample was also affected, with the highest sperm concentration observed at 34C, followed by 38C, and the lowest at 40C, indicating that higher temperatures likely due to increased oxidative stress and metabolic exhaustion are not favourable for canine sperm. As conclusion, the results showed that canine spermatozoa exhibit temperature-dependent survival patterns, with an optimal range between 34C and 38C. The thermotaxis for spermatozoa may play a role in natural sperm selection, influencing fertilization efficiency. Future studies should be set up to investigate the molecular mechanisms underlying thermotaxis and its potential use in sperm selection for improved fertility outcomes.

 Sperm transfer through the reproductive tract is crucial for successful fertilization in most of mammals. After ejaculation, spermatozoa need to go through a complex physiological barrier and environments in the reproductive tract. Also, sperm must respond to many environmental conditions, including temperature. The present study aims to investigate the thermotactic response of sperms collected from American Bully dogs and its impact on motility, viability, membrane integrity, and concentration at different temperatures. Evaluated semen samples, collected from mature dogs incubated at 34C, 38C, and 40C for 60 minutes in a custom-designed thermotaxis model. Some sperm parameters were assessed using computer-assisted sperm analysis iSperm CASA, Nigrosin-Eosin staining, and the Hypo-Osmotic Swelling Test HOST. Results showed that sperm motility and concentration were increased at 34C and 38C, while exposure to 40C resulted in a significant decline in motility, progressive motility, and concentration. Slightly higher curvilinear velocity VCL and straight-line velocity VSL at 38C suggests optimal motility at this temperature. Membrane integrity HOST-positive sperm was significantly compromised at 40C, with a corresponding decrease in viability, as confirmed by Nigrosin-Eosin staining. Sperm direction towards to different temperature for the sample was also affected, with the highest sperm concentration observed at 34C, followed by 38C, and the lowest at 40C, indicating that higher temperatures likely due to increased oxidative stress and metabolic exhaustion are not favourable for canine sperm. As conclusion, the results showed that canine spermatozoa exhibit temperature-dependent survival patterns, with an optimal range between 34C and 38C. The thermotaxis for spermatozoa may play a role in natural sperm selection, influencing fertilization efficiency. Future studies should be set up to investigate the molecular mechanisms underlying thermotaxis and its potential use in sperm selection for improved fertility outcomes.

 Sperm transfer through the reproductive tract is crucial for successful fertilization in most of mammals. After ejaculation, spermatozoa need to go through a complex physiological barrier and environments in the reproductive tract. Also, sperm must respond to many environmental conditions, including temperature. The present study aims to investigate the thermotactic response of sperms collected from American Bully dogs and its impact on motility, viability, membrane integrity, and concentration at different temperatures. Evaluated semen samples, collected from mature dogs incubated at 34C, 38C, and 40C for 60 minutes in a custom-designed thermotaxis model. Some sperm parameters were assessed using computer-assisted sperm analysis iSperm CASA, Nigrosin-Eosin staining, and the Hypo-Osmotic Swelling Test HOST. Results showed that sperm motility and concentration were increased at 34C and 38C, while exposure to 40C resulted in a significant decline in motility, progressive motility, and concentration. Slightly higher curvilinear velocity VCL and straight-line velocity VSL at 38C suggests optimal motility at this temperature. Membrane integrity HOST-positive sperm was significantly compromised at 40C, with a corresponding decrease in viability, as confirmed by Nigrosin-Eosin staining. Sperm direction towards to different temperature for the sample was also affected, with the highest sperm concentration observed at 34C, followed by 38C, and the lowest at 40C, indicating that higher temperatures likely due to increased oxidative stress and metabolic exhaustion are not favourable for canine sperm. As conclusion, the results showed that canine spermatozoa exhibit temperature-dependent survival patterns, with an optimal range between 34C and 38C. The thermotaxis for spermatozoa may play a role in natural sperm selection, influencing fertilization efficiency. Future studies should be set up to investigate the molecular mechanisms underlying thermotaxis and its potential use in sperm selection for improved fertility outcomes.

 Memelilerin çoğunda başarılı döllenme için sperm taşınımı, üreme kanalında kritik bir süreçtir. Ejakülasyondan sonra spermatozoonlar, üreme kanalındaki karmaşık fizyolojik bariyerleri ve farklı çevresel koşulları aşmak zorundadır. Ayrıca, spermatozoonların sıcaklık da dahil olmak üzere birçok çevresel faktöre yanıt vermesi gerekmektedir. Bu çalışma, American Bully ırkı köpeklerden toplanan spermatozoonların termotaktik tepkisini incelemeyi ve farklı sıcaklıklarda 32C, 38C ve 40C inkübasyonun motilite, canlılık, membran bütünlüğü ve konsantrasyon üzerindeki etkisini değerlendirmeyi amaçlamaktadır. Değerlendirilen semen örnekleri, olgun erkek köpeklerden toplanmış ve özel olarak tasarlanmış bir termotaksis modeli içinde 60 dakika boyunca inkübe edilmiştir. Çeşitli sperm parametreleri, bilgisayar destekli sperm analiz sistemi iSperm CASA, Nigrosin-Eosin boyama ve Hipo-Ozmotik Şişme Testi HOST kullanılarak değerlendirilmiştir. Sonuçlar, 32C ve 38C sıcaklıklarında sperm motilitesi ve konsantrasyonunda artış olduğunu, ancak 40Cye maruz kalmanın motilite, progresif motilite ve konsantrasyonda önemli bir düşüşe yol açtığını göstermiştir. 38Cde gözlemlenen daha yüksek eğrisel hız VCL ve düz çizgi hızı VSL, bu sıcaklığın optimal motiliteyi desteklediğini göstermektedir. Membran bütünlüğü HOST-pozitif sperm oranı, 40Cde önemli ölçüde bozulmuş ve bu durum Nigrosin-Eosin boyaması ile doğrulanan canlılık azalması ile paralellik göstermiştir. Örneklerde sperm yönelimi de sıcaklığa bağlı olarak değişmiş, en yüksek sperm konsantrasyonu 32Cde, ardından 38Cde gözlenirken, en düşük konsantrasyon 40Cde tespit edilmiştir. Bu durum, yüksek sıcaklıkların artan oksidatif stres ve metabolik tükenme nedeniyle köpek spermatozoonları için elverişli olmadığını düşündürmektedir. Sonuç olarak, bu çalışma köpek spermatozoonlarının sıcaklığa bağlı olarak hayatta kalma modelleri sergilediğini ve optimal sıcaklık aralığının 32C ile 38C arasında olduğunu göstermektedir. Spermatozoonların termotaksisi, doğal sperm seçiliminde önemli bir rol oynayabilir ve döllenme başarısını etkileyebilir. Gelecekteki çalışmalar, termotaksisin moleküler mekanizmalarını araştırarak, bu olgunun fertiliteyi artırmaya yönelik sperm seçiminde nasıl kullanılabileceğini belirlemeye odaklanmalıdır.

Makale detayları

Temperature-Dependent Thermotaxis and Its Impact on Motility, Viability, and Concentration in Canine (American Bully) Sperm

Yazarlar:

Ali Galip Önal

Yayıncı:

Cukurova University

Ana Başlıklar:

Zootekni | Genetik ve Biyoistatistik

Dergi:

Çukurova Tarım ve Gıda Bilimleri Dergisi Vol.40 Issue 1, pp.140-147

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001353359

pISSN:

2636-7874

Termotaxis

canine sperm

sperm kinetics

viabilityTermotaxi

köpek sperm

sperm kinetik

Sayı

Makale detayları

Temperature-Dependent Thermotaxis and Its Impact on Motility, Viability, and Concentration in Canine (American Bully) Sperm

Yazarlar: Ali Galip Önal

Yayıncı: Cukurova University

Ana Başlıklar: Zootekni | Genetik ve Biyoistatistik

Dergi: Çukurova Tarım ve Gıda Bilimleri Dergisi Vol.40 Issue 1, pp.140-147

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001353359

pISSN: 2636-7874

Termotaxis

canine sperm

sperm kinetics

viabilityTermotaxi

köpek sperm

sperm kinetik

Sayı

Yazarlar:

Ali Galip Önal

Yayıncı:

Cukurova University

Ana Başlıklar:

Zootekni | Genetik ve Biyoistatistik

Dergi:

Çukurova Tarım ve Gıda Bilimleri Dergisi Vol.40 Issue 1, pp.140-147

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001353359

pISSN:

2636-7874