The Investigation Protective Effect of Tarantula Cubensis Extract in Rats Induced Experimental Gentamicin Nephrotoxicity

Dilek Akşit

Makale detayları

The Investigation Protective Effect of Tarantula Cubensis Extract in Rats Induced Experimental Gentamicin Nephrotoxicity

Yazarlar:

Dilek Akşit

Yayıncı:

Aydin Adnan Menderes University

Ana Başlıklar:

Veteriner Cerrahi

Dergi:

Animal Health Production and Hygiene Vol.12 Issue 1, pp.31-39

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001292572

pISSN:

2146-7269

Anahtar kelimeler:

Özet:

 Gentamicin GM, which is used in the treatment of infections caused by gram-negative bacteria, has limited clinical use due to its serious nephrotoxic side effects. Tarantula cubensis extract TCE is a homeopathic product that is widely used and proven to be effective in veterinary medicine to benefit from its regeneration, demarcation, antiphlogistic and resorptive effects. The aim of the study was to investigate the possible protective effects of TCE against these negative effects of Gentamicin, which is one of the drugs that trigger the formation of free radicals in the body, in terms of oxidative stress, apoptosis and antioxidant parameters. In this study, rats were divided into four equal groups. Groups; Control, GM, TCE, GMTCE. Blood and kidney tissue samples were taken 24 hours after the last injection. Urea and creatinine analysis were performed in serum, MDA, SOD and TAS analysis were performed in kidney and serum samples. Bcl-2 and Bax analyzes and histopathological evaluations were performed in the kidney tissue. An increase in MDA, creatinine and urea levels, and a decrease in TAS and SOD levels were observed in the GM group compared to the control group. On the other hand, in the GMTCE group, a decrease was observed in increasing parameters compared to the GM group, and an increase in TAS and SOD levels was observed. In the histopathological and immunohistochemical examination of kidney tissue, it was determined that pathological disorders and increased apoptosis decrease in Bcl-2, increase in Bax in the GM group decreased with TCE application. In conclusion, in the light of the data in this study, we believe that high-dose gentamicin causes side effects in the kidneys, while TCE may have antioxidant, antiapoptotic, protective and curative effects. However, additional studies are needed to confirm this assumption.

 Gentamicin GM, which is used in the treatment of infections caused by gram-negative bacteria, has limited clinical use due to its serious nephrotoxic side effects. Tarantula cubensis extract TCE is a homeopathic product that is widely used and proven to be effective in veterinary medicine to benefit from its regeneration, demarcation, antiphlogistic and resorptive effects. The aim of the study was to investigate the possible protective effects of TCE against these negative effects of Gentamicin, which is one of the drugs that trigger the formation of free radicals in the body, in terms of oxidative stress, apoptosis and antioxidant parameters. In this study, rats were divided into four equal groups. Groups; Control, GM, TCE, GMTCE. Blood and kidney tissue samples were taken 24 hours after the last injection. Urea and creatinine analysis were performed in serum, MDA, SOD and TAS analysis were performed in kidney and serum samples. Bcl-2 and Bax analyzes and histopathological evaluations were performed in the kidney tissue. An increase in MDA, creatinine and urea levels, and a decrease in TAS and SOD levels were observed in the GM group compared to the control group. On the other hand, in the GMTCE group, a decrease was observed in increasing parameters compared to the GM group, and an increase in TAS and SOD levels was observed. In the histopathological and immunohistochemical examination of kidney tissue, it was determined that pathological disorders and increased apoptosis decrease in Bcl-2, increase in Bax in the GM group decreased with TCE application. In conclusion, in the light of the data in this study, we believe that high-dose gentamicin causes side effects in the kidneys, while TCE may have antioxidant, antiapoptotic, protective and curative effects. However, additional studies are needed to confirm this assumption.

 Gentamicin GM, which is used in the treatment of infections caused by gram-negative bacteria, has limited clinical use due to its serious nephrotoxic side effects. Tarantula cubensis extract TCE is a homeopathic product that is widely used and proven to be effective in veterinary medicine to benefit from its regeneration, demarcation, antiphlogistic and resorptive effects. The aim of the study was to investigate the possible protective effects of TCE against these negative effects of Gentamicin, which is one of the drugs that trigger the formation of free radicals in the body, in terms of oxidative stress, apoptosis and antioxidant parameters. In this study, rats were divided into four equal groups. Groups; Control, GM, TCE, GMTCE. Blood and kidney tissue samples were taken 24 hours after the last injection. Urea and creatinine analysis were performed in serum, MDA, SOD and TAS analysis were performed in kidney and serum samples. Bcl-2 and Bax analyzes and histopathological evaluations were performed in the kidney tissue. An increase in MDA, creatinine and urea levels, and a decrease in TAS and SOD levels were observed in the GM group compared to the control group. On the other hand, in the GMTCE group, a decrease was observed in increasing parameters compared to the GM group, and an increase in TAS and SOD levels was observed. In the histopathological and immunohistochemical examination of kidney tissue, it was determined that pathological disorders and increased apoptosis decrease in Bcl-2, increase in Bax in the GM group decreased with TCE application. In conclusion, in the light of the data in this study, we believe that high-dose gentamicin causes side effects in the kidneys, while TCE may have antioxidant, antiapoptotic, protective and curative effects. However, additional studies are needed to confirm this assumption.

 Gentamicin GM, which is used in the treatment of infections caused by gram-negative bacteria, has limited clinical use due to its serious nephrotoxic side effects. Tarantula cubensis extract TCE is a homeopathic product that is widely used and proven to be effective in veterinary medicine to benefit from its regeneration, demarcation, antiphlogistic and resorptive effects. The aim of the study was to investigate the possible protective effects of TCE against these negative effects of Gentamicin, which is one of the drugs that trigger the formation of free radicals in the body, in terms of oxidative stress, apoptosis and antioxidant parameters. In this study, rats were divided into four equal groups. Groups; Control, GM, TCE, GMTCE. Blood and kidney tissue samples were taken 24 hours after the last injection. Urea and creatinine analysis were performed in serum, MDA, SOD and TAS analysis were performed in kidney and serum samples. Bcl-2 and Bax analyzes and histopathological evaluations were performed in the kidney tissue. An increase in MDA, creatinine and urea levels, and a decrease in TAS and SOD levels were observed in the GM group compared to the control group. On the other hand, in the GMTCE group, a decrease was observed in increasing parameters compared to the GM group, and an increase in TAS and SOD levels was observed. In the histopathological and immunohistochemical examination of kidney tissue, it was determined that pathological disorders and increased apoptosis decrease in Bcl-2, increase in Bax in the GM group decreased with TCE application. In conclusion, in the light of the data in this study, we believe that high-dose gentamicin causes side effects in the kidneys, while TCE may have antioxidant, antiapoptotic, protective and curative effects. However, additional studies are needed to confirm this assumption.

Makale detayları

The Investigation Protective Effect of Tarantula Cubensis Extract in Rats Induced Experimental Gentamicin Nephrotoxicity

Yazarlar:

Dilek Akşit

Yayıncı:

Aydin Adnan Menderes University

Ana Başlıklar:

Veteriner Cerrahi

Dergi:

Animal Health Production and Hygiene Vol.12 Issue 1, pp.31-39

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001292572

pISSN:

2146-7269

Antioxidant

apoptosis

gentamicin

nephrotoxicity

Tarantula cubensis extract.

Sayı

Makale detayları

The Investigation Protective Effect of Tarantula Cubensis Extract in Rats Induced Experimental Gentamicin Nephrotoxicity

Yazarlar: Dilek Akşit

Yayıncı: Aydin Adnan Menderes University

Ana Başlıklar: Veteriner Cerrahi

Dergi: Animal Health Production and Hygiene Vol.12 Issue 1, pp.31-39

Belge Tipi: Makale

Yayın Tarihi: 6/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001292572

pISSN: 2146-7269

Antioxidant

apoptosis

gentamicin

nephrotoxicity

Tarantula cubensis extract.

Sayı

Yazarlar:

Dilek Akşit

Yayıncı:

Aydin Adnan Menderes University

Ana Başlıklar:

Veteriner Cerrahi

Dergi:

Animal Health Production and Hygiene Vol.12 Issue 1, pp.31-39

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001292572

pISSN:

2146-7269