Characterization of Cryphonectria parasitica From Native Chestnut Trees in Kaz Mountain (Mount Ida)

Hilal ÖZKILIÇ; Zerrin KOZMA; Burcu PEKDEMİR; Birgül GÜLNAR

doi:https://doi.org/10.13002/jafag4357

Makale detayları

Characterization of Cryphonectria parasitica From Native Chestnut Trees in Kaz Mountain (Mount Ida)

Yazarlar:

Hilal ÖZKILIÇ | Zerrin KOZMA | Burcu PEKDEMİR | Birgül GÜLNAR

Yayıncı:

Tokat Gaziosmanpasa University

Dergi:

Journal of Agricultural Faculty of Gaziosmanpaşa University (JAFAG) 2018 Vol.35 Issue 1, pp.38-45

Belge Tipi:

Makale

Yayın Tarihi:

4/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000449270

Sıklık:

Üç Yıllık

eISSN:

2147-8848

pISSN:

1300-2910

DOI:

https://doi.org/10.13002/jafag4357

Özet:

 

Cryphonectria parasitica is a causal agent of chestnut blight worldwide and it dramatically affects natural chestnut trees in Kaz Mountain (Mount Ida). Different biological control approaches have been proposed to constrain the disease. The pathogen has mating system with two alternate idiomorphs which may increase pathogen genetic variability due the recombination events. In this study, C. parasitica isolates were diagnosed with partial sequencing of Translation Elongation Factor-1α (Ef-1α) gene, tested for their pathogenicity, evaluated for their mating types, and the isolates of Trichoderma sp. which were isolated from the same lesion where some isolates of C. parasitica were obtained were investigated for antagonistic effect on C. parasitica. It was not detected any mutation in sequenced part of Ef-1α gene. All the isolates were confirmed as pathogen in in vitro assays by inoculation to apple fruit. Only one mating type (Mat1-1) was detected for some of the isolates. Trichoderma sp. isolates were suppressed in vitro growth rate of C. parasitica. Both C. parasitica and Trichoderma sp. caused necrotic symptoms on the fruits once they were inoculated on apple, but, Trichoderma sp. did not affect lesion development caused by C. parasitica. Overall results present that wild chestnut trees in Kaz Mountain are under threat of chestnut blight pathogens having similar aggressiveness and genetic identity and, isolates of Trichoderma sp. obtained in this study should be assessed by in vivo experiments for their biocontrol ability against to C. parasitica.

Makale detayları

Characterization of Cryphonectria parasitica From Native Chestnut Trees in Kaz Mountain (Mount Ida)

Yazarlar: Hilal ÖZKILIÇ | Zerrin KOZMA | Burcu PEKDEMİR | Birgül GÜLNAR

Yayıncı: Tokat Gaziosmanpasa University

Dergi: Journal of Agricultural Faculty of Gaziosmanpaşa University (JAFAG) 2018 Vol.35 Issue 1, pp.38-45

Belge Tipi: Makale

Yayın Tarihi: 4/2018

Belge Dili: İngilizce

Document ID (Org No.): 2000449270

Sıklık: Üç Yıllık

eISSN: 2147-8848

pISSN: 1300-2910

DOI: https://doi.org/10.13002/jafag4357

Sayı

Yazarlar:

Hilal ÖZKILIÇ | Zerrin KOZMA | Burcu PEKDEMİR | Birgül GÜLNAR

Yayıncı:

Tokat Gaziosmanpasa University

Dergi:

Journal of Agricultural Faculty of Gaziosmanpaşa University (JAFAG) 2018 Vol.35 Issue 1, pp.38-45

Belge Tipi:

Makale

Yayın Tarihi:

4/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000449270

Sıklık:

Üç Yıllık

eISSN:

2147-8848

pISSN:

1300-2910

DOI:

https://doi.org/10.13002/jafag4357