Anti-Cancer and Antimicrobial Properties of St. John's Wort (Hypericum perforatum)

Hüseyin Karci

Makale detayları

Anti-Cancer and Antimicrobial Properties of St. John's Wort (Hypericum perforatum)

Yazarlar:

Hüseyin Karci

Yayıncı:

Inonu University

Ana Başlıklar:

Gıda Özellikleri

Dergi:

İnönü Üniversitesi Sağlık Hizmetleri Meslek Yüksek Okulu Dergisi Vol.13 Issue 1, pp.181-191

Belge Tipi:

Makale

Yayın Tarihi:

2/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001340804

pISSN:

2147-7892

Anahtar kelimeler:

Özet:

 St. Johns Wort Hypericum perforatum is a medicinal plant widely used in traditional medicine, known for its significant pharmacological properties, including antimicrobial and antitumor activities. In this study, extracts of Hypericum perforatum were prepared using the maceration method from plants collected at the foothills of Kubbe Mountain, located in the Pütürge district of Malatya province. These extracts were evaluated for their antimicrobial and anticancer activities. Antimicrobial activity was investigated against microorganisms, including Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 29213, Pseudomonas aeruginosa ATCC 27853, Candida albicans SC5314ATCC MYA-2876, and Candida glabrata ATCC 2001, using the Disk Diffusion Test. Anticancer activity was assessed in HCT116 human colon cancer, SH-SY5Y human brain cancer, and BEAS-2B human healthy lung cell lines using the MTT assay, with IC50 values compared to those of the standard anticancer drug, Cisplatin. Regarding antimicrobial activity, the inhibition zones were measured as 9.330.78 mm for E. coli, 12.170.53 mm for P. aeruginosa, and 11.730.41 mm for S. aureus. No inhibition was observed for the yeasts C. albicans and C. glabrata. Low cytotoxicity was observed in the healthy cell line BEAS-2B, with an IC50 value of 48.87  7.03 μgmL, whereas high cytotoxicity was detected in cancer cell lines, with IC50 values of 23.273.35 μgmL for SH-SY5Y and 10.673.05 μgmL for HCT116. These findings indicate that Hypericum perforatum extract can effectively target cancer cells while sparing healthy cells, suggesting its potential for selective toxicity. In conclusion, Hypericum perforatum extract demonstrated significant antimicrobial activity against P. aeruginosa and S. aureus, as well as strong cytotoxicity in cancer cell lines, highlighting its potential as a natural therapeutic agent.

 St. Johns Wort Hypericum perforatum is a medicinal plant widely used in traditional medicine, known for its significant pharmacological properties, including antimicrobial and antitumor activities. In this study, extracts of Hypericum perforatum were prepared using the maceration method from plants collected at the foothills of Kubbe Mountain, located in the Pütürge district of Malatya province. These extracts were evaluated for their antimicrobial and anticancer activities. Antimicrobial activity was investigated against microorganisms, including Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 29213, Pseudomonas aeruginosa ATCC 27853, Candida albicans SC5314ATCC MYA-2876, and Candida glabrata ATCC 2001, using the Disk Diffusion Test. Anticancer activity was assessed in HCT116 human colon cancer, SH-SY5Y human brain cancer, and BEAS-2B human healthy lung cell lines using the MTT assay, with IC50 values compared to those of the standard anticancer drug, Cisplatin. Regarding antimicrobial activity, the inhibition zones were measured as 9.330.78 mm for E. coli, 12.170.53 mm for P. aeruginosa, and 11.730.41 mm for S. aureus. No inhibition was observed for the yeasts C. albicans and C. glabrata. Low cytotoxicity was observed in the healthy cell line BEAS-2B, with an IC50 value of 48.87  7.03 μgmL, whereas high cytotoxicity was detected in cancer cell lines, with IC50 values of 23.273.35 μgmL for SH-SY5Y and 10.673.05 μgmL for HCT116. These findings indicate that Hypericum perforatum extract can effectively target cancer cells while sparing healthy cells, suggesting its potential for selective toxicity. In conclusion, Hypericum perforatum extract demonstrated significant antimicrobial activity against P. aeruginosa and S. aureus, as well as strong cytotoxicity in cancer cell lines, highlighting its potential as a natural therapeutic agent.

 St. Johns Wort Hypericum perforatum is a medicinal plant widely used in traditional medicine, known for its significant pharmacological properties, including antimicrobial and antitumor activities. In this study, extracts of Hypericum perforatum were prepared using the maceration method from plants collected at the foothills of Kubbe Mountain, located in the Pütürge district of Malatya province. These extracts were evaluated for their antimicrobial and anticancer activities. Antimicrobial activity was investigated against microorganisms, including Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 29213, Pseudomonas aeruginosa ATCC 27853, Candida albicans SC5314ATCC MYA-2876, and Candida glabrata ATCC 2001, using the Disk Diffusion Test. Anticancer activity was assessed in HCT116 human colon cancer, SH-SY5Y human brain cancer, and BEAS-2B human healthy lung cell lines using the MTT assay, with IC50 values compared to those of the standard anticancer drug, Cisplatin. Regarding antimicrobial activity, the inhibition zones were measured as 9.330.78 mm for E. coli, 12.170.53 mm for P. aeruginosa, and 11.730.41 mm for S. aureus. No inhibition was observed for the yeasts C. albicans and C. glabrata. Low cytotoxicity was observed in the healthy cell line BEAS-2B, with an IC50 value of 48.87  7.03 μgmL, whereas high cytotoxicity was detected in cancer cell lines, with IC50 values of 23.273.35 μgmL for SH-SY5Y and 10.673.05 μgmL for HCT116. These findings indicate that Hypericum perforatum extract can effectively target cancer cells while sparing healthy cells, suggesting its potential for selective toxicity. In conclusion, Hypericum perforatum extract demonstrated significant antimicrobial activity against P. aeruginosa and S. aureus, as well as strong cytotoxicity in cancer cell lines, highlighting its potential as a natural therapeutic agent.

 Sarı kantaron Hypericum perforatum, geleneksel tıpta yaygın olarak kullanılan ve antimikrobiyal ile antitümör gibi önemli farmakolojik özellikler sergileyen bir bitkidir. Bu çalışmada, Malatya ili Pütürge ilçesi Kubbe Dağı eteklerinden toplanan sarı kantaron bitkisinden maserasyon yöntemiyle ekstre hazırlanmış, antimikrobiyal ve antikanser aktiviteleri açısından değerlendirilmiştir. Disk Difüzyon Testi kullanılarak Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 29213, Pseudomonas aeruginosa ATCC 27853, Candida albicans SC5314ATCC MYA-2876 ve Candida glabrata ATCC 2001 gibi mikroorganizmalar üzerindeki antimikrobiyal etkinliği araştırılmıştır. Antikanser aktivitek, MTT testiyle HCT116 insan kolon kanseri, SH-SY5Y insan beyin kanseri ve BEAS-2B insan sağlıklı akciğer hücresi hücre hatlarında değerlendirilmiş ve elde edilen IC50 değerleri, standart antikanser ilacı Cisplatin ile karşılaştırılmıştır. Antimikrobiyal aktivite için, inhibisyon bölgeleri incelendiğinde C. albicans ve C. glabrata mayalarında inhibisyon göstermemiş, E. colide 9,330,78 mm, P. aeruginosada 12.170,53 mm ve S. aureusta 11.730.41 mm olarak hesaplanmıştır. Antkanser aktiviteleri ise sağlıklı hücre hattı BEAS-2B hücrelerinde 48.877.03 μgmL, IC50 değeriyle düşük sitotoksisite, kanserli hücre hatlarında IC50 değerleri SH-SY5Y hücre hattında 23.273.35 μgmL, HCT116 hücre hattında ise 10.673.05 μgmL değerleriyle yüksek sitotoksisiste tespit edilmiştir. Sarı kantaron ekstresi sağlıklı hücrelere zarar vermeden kanser hücrelerini etkili bir şekilde hedef alabildiğini ve seçici toksisiteye sahip olabileceğini göstermektedir. Sonuç olarak; P. aeruginosa ve S. aureusa karşı etkili antimikrobiyal aktivite ve kanserli hücre hatlarında güçlü sitotoksisite sergileyerek doğal tedavi ajanı olarak değerlendirilebilecek bir potansiyel ortaya koymuştur.

Makale detayları

Anti-Cancer and Antimicrobial Properties of St. John's Wort (Hypericum perforatum)

Yazarlar:

Hüseyin Karci

Yayıncı:

Inonu University

Ana Başlıklar:

Gıda Özellikleri

Dergi:

İnönü Üniversitesi Sağlık Hizmetleri Meslek Yüksek Okulu Dergisi Vol.13 Issue 1, pp.181-191

Belge Tipi:

Makale

Yayın Tarihi:

2/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001340804

pISSN:

2147-7892

Anticancer

Antimicrobial

Disk diffusion test

MTT

St. JohnAntikanser

Antimikrobiyal

Disk difüzyon testi

MTT

Sarı kantaron

Sayı

Makale detayları

Yazarlar: Hüseyin Karci

Yayıncı: Inonu University

Ana Başlıklar: Gıda Özellikleri

Dergi: İnönü Üniversitesi Sağlık Hizmetleri Meslek Yüksek Okulu Dergisi Vol.13 Issue 1, pp.181-191

Belge Tipi: Makale

Yayın Tarihi: 2/2025

Belge Dili: Türkçe

Document ID (Org No.): 2001340804

pISSN: 2147-7892

Sayı

Yazarlar:

Hüseyin Karci

Yayıncı:

Inonu University

Ana Başlıklar:

Gıda Özellikleri

Dergi:

İnönü Üniversitesi Sağlık Hizmetleri Meslek Yüksek Okulu Dergisi Vol.13 Issue 1, pp.181-191

Belge Tipi:

Makale

Yayın Tarihi:

2/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001340804

pISSN:

2147-7892