Determination of tramadol in pharmaceutical preparations by GC-MS method

Bilal Yılmaz; Emrah Alkan

Makale detayları

Determination of tramadol in pharmaceutical preparations by GC-MS method

Yazarlar:

Bilal Yılmaz | Emrah Alkan

Yayıncı:

Ataturk University

Ana Başlıklar:

Micro and Nanosystems

Dergi:

NanoEra Vol.5 Issue 1, pp.22-27

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001351744

pISSN:

2792-0666

Anahtar kelimeler:

Özet:

 In this study, a gas chromatography-mass spectrometry GC-MS method was developed to analyze tramadol in both pure form and pharmaceutical formulations. Diclofenac served as the internal standard. The linearity of the GC-MS method was validated within a concentration range of 0.05-5.0 μg mL-1. The intra- and inter-day relative standard deviations were found to be below 1.18 and 3.51, respectively. The detection and quantification limits for the GC-MS method were determined to be 0.015 and 0.045 μg mL-1, respectively. Stability testing revealed that tramadol remained stable in solutions after 24 hours of incubation at room temperature or after 72 hours of storage at 4 C and -20C. Under the selected assay conditions, tablet excipients did not cause any interference. Furthermore, the method proved to be effective in quantifying tramadol and ensuring the consistency of formulation content in commercial tramadol dosage forms.

 In this study, a gas chromatography-mass spectrometry GC-MS method was developed to analyze tramadol in both pure form and pharmaceutical formulations. Diclofenac served as the internal standard. The linearity of the GC-MS method was validated within a concentration range of 0.05-5.0 μg mL-1. The intra- and inter-day relative standard deviations were found to be below 1.18 and 3.51, respectively. The detection and quantification limits for the GC-MS method were determined to be 0.015 and 0.045 μg mL-1, respectively. Stability testing revealed that tramadol remained stable in solutions after 24 hours of incubation at room temperature or after 72 hours of storage at 4 C and -20C. Under the selected assay conditions, tablet excipients did not cause any interference. Furthermore, the method proved to be effective in quantifying tramadol and ensuring the consistency of formulation content in commercial tramadol dosage forms.

 In this study, a gas chromatography-mass spectrometry GC-MS method was developed to analyze tramadol in both pure form and pharmaceutical formulations. Diclofenac served as the internal standard. The linearity of the GC-MS method was validated within a concentration range of 0.05-5.0 μg mL-1. The intra- and inter-day relative standard deviations were found to be below 1.18 and 3.51, respectively. The detection and quantification limits for the GC-MS method were determined to be 0.015 and 0.045 μg mL-1, respectively. Stability testing revealed that tramadol remained stable in solutions after 24 hours of incubation at room temperature or after 72 hours of storage at 4 C and -20C. Under the selected assay conditions, tablet excipients did not cause any interference. Furthermore, the method proved to be effective in quantifying tramadol and ensuring the consistency of formulation content in commercial tramadol dosage forms.

 In this study, a gas chromatography-mass spectrometry GC-MS method was developed to analyze tramadol in both pure form and pharmaceutical formulations. Diclofenac served as the internal standard. The linearity of the GC-MS method was validated within a concentration range of 0.05-5.0 μg mL-1. The intra- and inter-day relative standard deviations were found to be below 1.18 and 3.51, respectively. The detection and quantification limits for the GC-MS method were determined to be 0.015 and 0.045 μg mL-1, respectively. Stability testing revealed that tramadol remained stable in solutions after 24 hours of incubation at room temperature or after 72 hours of storage at 4 C and -20C. Under the selected assay conditions, tablet excipients did not cause any interference. Furthermore, the method proved to be effective in quantifying tramadol and ensuring the consistency of formulation content in commercial tramadol dosage forms.

Makale detayları

Determination of tramadol in pharmaceutical preparations by GC-MS method

Yazarlar:

Bilal Yılmaz | Emrah Alkan

Yayıncı:

Ataturk University

Ana Başlıklar:

Micro and Nanosystems

Dergi:

NanoEra Vol.5 Issue 1, pp.22-27

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001351744

pISSN:

2792-0666

Tramadol

GC-MS

Validation

Tablet

Sayı

Makale detayları

Determination of tramadol in pharmaceutical preparations by GC-MS method

Yazarlar: Bilal Yılmaz | Emrah Alkan

Yayıncı: Ataturk University

Ana Başlıklar: Micro and Nanosystems

Dergi: NanoEra Vol.5 Issue 1, pp.22-27

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001351744

pISSN: 2792-0666

Tramadol

GC-MS

Validation

Tablet

Sayı

Yazarlar:

Bilal Yılmaz | Emrah Alkan

Yayıncı:

Ataturk University

Ana Başlıklar:

Micro and Nanosystems

Dergi:

NanoEra Vol.5 Issue 1, pp.22-27

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001351744

pISSN:

2792-0666