Validation of an UPLC method for the determination of Pioglitazone Hydrochloride active substance in Pharmaceutical Dosage Forms

Sinem Bilgiç Karaağaç; Dilek Bilgiç Alkaya; Serap Ayaz Seyhan; Buşra Nagihan Öztürk

Makale detayları

Validation of an UPLC method for the determination of Pioglitazone Hydrochloride active substance in Pharmaceutical Dosage Forms

Yazarlar:

Sinem Bilgiç Karaağaç | Dilek Bilgiç Alkaya | Serap Ayaz Seyhan | Buşra Nagihan Öztürk

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Delivery Technologies

Dergi:

Journal of Research in Pharmacy Vol.28 Issue 1, pp.385-395

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346005

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 The present work reports an ultra-performance liquid chromatography RP-UPLC method for the quantitative determination of pioglitazone HCl PGZ in pharmaceutical dosage form. The chromatographic separation was performed on C18, BEH 50 mm x 2.1 mm, 1.7 μm column using isocratic elution. The optimized mobile phase consists of phosphate buffer pH:6 as a solvent-A and 55:45 vv mixture of acetonitrile as solvent-B. Flow rate was 0.4 mlmin with UV detection at λmax 225 nm and the injection volume was set at 2 μL with retention time 1 min. The developed RP-UPLC method was validated as per International Conference on Harmonization ICH guidelines with respect to system suitability, specificity, precision, accuracy, linearity.

 The present work reports an ultra-performance liquid chromatography RP-UPLC method for the quantitative determination of pioglitazone HCl PGZ in pharmaceutical dosage form. The chromatographic separation was performed on C18, BEH 50 mm x 2.1 mm, 1.7 μm column using isocratic elution. The optimized mobile phase consists of phosphate buffer pH:6 as a solvent-A and 55:45 vv mixture of acetonitrile as solvent-B. Flow rate was 0.4 mlmin with UV detection at λmax 225 nm and the injection volume was set at 2 μL with retention time 1 min. The developed RP-UPLC method was validated as per International Conference on Harmonization ICH guidelines with respect to system suitability, specificity, precision, accuracy, linearity.

 The present work reports an ultra-performance liquid chromatography RP-UPLC method for the quantitative determination of pioglitazone HCl PGZ in pharmaceutical dosage form. The chromatographic separation was performed on C18, BEH 50 mm x 2.1 mm, 1.7 μm column using isocratic elution. The optimized mobile phase consists of phosphate buffer pH:6 as a solvent-A and 55:45 vv mixture of acetonitrile as solvent-B. Flow rate was 0.4 mlmin with UV detection at λmax 225 nm and the injection volume was set at 2 μL with retention time 1 min. The developed RP-UPLC method was validated as per International Conference on Harmonization ICH guidelines with respect to system suitability, specificity, precision, accuracy, linearity.

 The present work reports an ultra-performance liquid chromatography RP-UPLC method for the quantitative determination of pioglitazone HCl PGZ in pharmaceutical dosage form. The chromatographic separation was performed on C18, BEH 50 mm x 2.1 mm, 1.7 μm column using isocratic elution. The optimized mobile phase consists of phosphate buffer pH:6 as a solvent-A and 55:45 vv mixture of acetonitrile as solvent-B. Flow rate was 0.4 mlmin with UV detection at λmax 225 nm and the injection volume was set at 2 μL with retention time 1 min. The developed RP-UPLC method was validated as per International Conference on Harmonization ICH guidelines with respect to system suitability, specificity, precision, accuracy, linearity.

Makale detayları

Validation of an UPLC method for the determination of Pioglitazone Hydrochloride active substance in Pharmaceutical Dosage Forms

Yazarlar:

Sinem Bilgiç Karaağaç | Dilek Bilgiç Alkaya | Serap Ayaz Seyhan | Buşra Nagihan Öztürk

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Delivery Technologies

Dergi:

Journal of Research in Pharmacy Vol.28 Issue 1, pp.385-395

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346005

pISSN:

2630-6344

Diabetes

Pioglitazone hydrochloride

UPLC

Validation

Pharmaceutical Dosage Form

Sayı

Makale detayları

Validation of an UPLC method for the determination of Pioglitazone Hydrochloride active substance in Pharmaceutical Dosage Forms

Yazarlar: Sinem Bilgiç Karaağaç | Dilek Bilgiç Alkaya | Serap Ayaz Seyhan | Buşra Nagihan Öztürk

Yayıncı: Marmara University

Ana Başlıklar: Pharmaceutical Delivery Technologies

Dergi: Journal of Research in Pharmacy Vol.28 Issue 1, pp.385-395

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001346005

pISSN: 2630-6344

Diabetes

Pioglitazone hydrochloride

UPLC

Validation

Pharmaceutical Dosage Form

Sayı

Yazarlar:

Sinem Bilgiç Karaağaç | Dilek Bilgiç Alkaya | Serap Ayaz Seyhan | Buşra Nagihan Öztürk

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Delivery Technologies

Dergi:

Journal of Research in Pharmacy Vol.28 Issue 1, pp.385-395

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346005

pISSN:

2630-6344