Esophageal replacement by hydroxylated bacterial cellulose patch in a rabbit model

Changlai ZHU; Fang LIU; Wenbo QIAN; Yingjie WANG; Qingsheng YOU; Tianyi ZHANG; Feng LI

Makale detayları

Esophageal replacement by hydroxylated bacterial cellulose patch in a rabbit model

Yazarlar:

Changlai ZHU | Fang LIU | Wenbo QIAN | Yingjie WANG | Qingsheng YOU | Tianyi ZHANG | Feng LI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2015 Vol.45 Issue 4, pp.762-770

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000752540

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Anahtar kelimeler:

Özet:

 

Background/aim: To repair esophageal defects by hydroxylated and kombucha -synthesized bacterial cellulose (HKBC) patch in a rabbit model.  Materials and methods: Semicircular esophageal defects 1 cm in length of the cervical esophagus were initially created in 18 Japanese big-ear rabbits and then repaired with HKBC patch grafts. The clinical outcomes including survival rate, weight change, food intake, and hematological and radiologic evaluation were observed. After X-ray evaluation, the rabbits were sacrificed sequentially at 1, 3, and 6 months for histopathologic analysis with light microscopy and scanning electron microscopy.  Results: Survival rate during the first month was 88.9% (n = 16). Two rabbits died from anastomotic leakage during the entire follow-up. Postoperatively, feeding function and body weight were gradually restored in the surviving animals. No hematological abnormalities were found, and no obvious anastomotic leakage, stenosis, or obstruction was observed under X-ray examination. The histopathologic results showed a progressive regeneration of the esophagus in the graft area, where the neo-esophagus tissue had characteristics similar to native esophageal tissue after 3 months of surgery.   Conclusion: HKBC is beneficial for esophageal tissue regeneration and may be a promising material for esophageal reconstruction.

 

Background/aim: To repair esophageal defects by hydroxylated and kombucha -synthesized bacterial cellulose (HKBC) patch in a rabbit model.  Materials and methods: Semicircular esophageal defects 1 cm in length of the cervical esophagus were initially created in 18 Japanese big-ear rabbits and then repaired with HKBC patch grafts. The clinical outcomes including survival rate, weight change, food intake, and hematological and radiologic evaluation were observed. After X-ray evaluation, the rabbits were sacrificed sequentially at 1, 3, and 6 months for histopathologic analysis with light microscopy and scanning electron microscopy.  Results: Survival rate during the first month was 88.9% (n = 16). Two rabbits died from anastomotic leakage during the entire follow-up. Postoperatively, feeding function and body weight were gradually restored in the surviving animals. No hematological abnormalities were found, and no obvious anastomotic leakage, stenosis, or obstruction was observed under X-ray examination. The histopathologic results showed a progressive regeneration of the esophagus in the graft area, where the neo-esophagus tissue had characteristics similar to native esophageal tissue after 3 months of surgery.   Conclusion: HKBC is beneficial for esophageal tissue regeneration and may be a promising material for esophageal reconstruction.

Makale detayları

Esophageal replacement by hydroxylated bacterial cellulose patch in a rabbit model

Yazarlar:

Changlai ZHU | Fang LIU | Wenbo QIAN | Yingjie WANG | Qingsheng YOU | Tianyi ZHANG | Feng LI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2015 Vol.45 Issue 4, pp.762-770

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000752540

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Artificial esophagus

kombucha

bacterial cellulose

biological material

Sayı

Makale detayları

Esophageal replacement by hydroxylated bacterial cellulose patch in a rabbit model

Yazarlar: Changlai ZHU | Fang LIU | Wenbo QIAN | Yingjie WANG | Qingsheng YOU | Tianyi ZHANG | Feng LI

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2015 Vol.45 Issue 4, pp.762-770

Belge Tipi: Makale

Yayın Tarihi: 8/2015

Belge Dili: Türkçe

Document ID (Org No.): 2000752540

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Artificial esophagus

kombucha

bacterial cellulose

biological material

Sayı

Yazarlar:

Changlai ZHU | Fang LIU | Wenbo QIAN | Yingjie WANG | Qingsheng YOU | Tianyi ZHANG | Feng LI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2015 Vol.45 Issue 4, pp.762-770

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000752540

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144