Identification and characterization of a seed-specific grapevine dehydrin involved in abiotic stress response within tolerant varieties

Mohsen HANANA; Samia DALDOUL; Romain FOUQUET; Laurent DELUC; Céline LEON

Makale detayları

Identification and characterization of a seed-specific grapevine dehydrin involved in abiotic stress response within tolerant varieties

Yazarlar:

Mohsen HANANA | Samia DALDOUL | Romain FOUQUET | Laurent DELUC | Céline LEON

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Botany 2014 Vol.38 Issue 6, pp.1157-1168

Belge Tipi:

Makale

Yayın Tarihi:

5/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000815981

Sıklık:

İki Aylık

eISSN:

1303-6106

pISSN:

1300-008X

Anahtar kelimeler:

Özet:

 

To identify and isolate genes related to abiotic stress (salinity and drought) tolerance in grapevine, a candidate gene approach led to the isolation from Cabernet Sauvignon cultivar of a full-length cDNA of dehydrin gene. The latter, named VvDhn, which is highly and mainly induced in late embryogenesis in seeds, encodes for a protein of 124 amino acids with a predicted molecular mass of 13.3 kDa. Details of the physicochemical parameters and structural properties (molecular mass, secondary structure, conserved domains and motives, and putative posttranslational modification sites) of the encoded protein have also been elucidated. The expression study of VvDhn was carried out within plant organs and tissues as well as under drought and salt stresses. VvDhn was not detected in vegetative tissue, whereas it was only expressed during seed development (during late embryogenesis) at extremely high levels and was induced by salt and drought stresses as well as ABA application. Moreover, salt stress induced VvDhn expression in the tolerant variety (Razegui) but not the sensitive variety (Syrah), which did not display expression variation during stress; VvDhn expression level and salt-stress response depend on regulatory mechanisms that are efficient only in the tolerant variety. On the other hand, under drought stress VvDhn was induced in both tolerant and sensitive varieties, with higher levels in the tolerant variety. In addition, stress signal molecules such as ABA (applied alone or in combination with saccharose) induced VvDhn expression, even at low levels. Minimal knowledge about the role and functionality of this gene is necessary and constitutes a prerequisite for including VvDhn in grapevine abiotic stress tolerance improvement programs.

 

To identify and isolate genes related to abiotic stress (salinity and drought) tolerance in grapevine, a candidate gene approach led to the isolation from Cabernet Sauvignon cultivar of a full-length cDNA of dehydrin gene. The latter, named VvDhn, which is highly and mainly induced in late embryogenesis in seeds, encodes for a protein of 124 amino acids with a predicted molecular mass of 13.3 kDa. Details of the physicochemical parameters and structural properties (molecular mass, secondary structure, conserved domains and motives, and putative posttranslational modification sites) of the encoded protein have also been elucidated. The expression study of VvDhn was carried out within plant organs and tissues as well as under drought and salt stresses. VvDhn was not detected in vegetative tissue, whereas it was only expressed during seed development (during late embryogenesis) at extremely high levels and was induced by salt and drought stresses as well as ABA application. Moreover, salt stress induced VvDhn expression in the tolerant variety (Razegui) but not the sensitive variety (Syrah), which did not display expression variation during stress; VvDhn expression level and salt-stress response depend on regulatory mechanisms that are efficient only in the tolerant variety. On the other hand, under drought stress VvDhn was induced in both tolerant and sensitive varieties, with higher levels in the tolerant variety. In addition, stress signal molecules such as ABA (applied alone or in combination with saccharose) induced VvDhn expression, even at low levels. Minimal knowledge about the role and functionality of this gene is necessary and constitutes a prerequisite for including VvDhn in grapevine abiotic stress tolerance improvement programs.

Makale detayları

Identification and characterization of a seed-specific grapevine dehydrin involved in abiotic stress response within tolerant varieties

Yazarlar:

Mohsen HANANA | Samia DALDOUL | Romain FOUQUET | Laurent DELUC | Céline LEON

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Botany 2014 Vol.38 Issue 6, pp.1157-1168

Belge Tipi:

Makale

Yayın Tarihi:

5/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000815981

Sıklık:

İki Aylık

eISSN:

1303-6106

pISSN:

1300-008X

Dehydrin

salinity

drought

expression study

ABA

activity regulation

bioinformatics

Sayı

Makale detayları

Yazarlar: Mohsen HANANA | Samia DALDOUL | Romain FOUQUET | Laurent DELUC | Céline LEON

Yayıncı: TUBITAK

Dergi: Turkish Journal of Botany 2014 Vol.38 Issue 6, pp.1157-1168

Belge Tipi: Makale

Yayın Tarihi: 5/2014

Belge Dili: Türkçe

Document ID (Org No.): 2000815981

Sıklık: İki Aylık

eISSN: 1303-6106

pISSN: 1300-008X

Sayı

Yazarlar:

Mohsen HANANA | Samia DALDOUL | Romain FOUQUET | Laurent DELUC | Céline LEON

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Botany 2014 Vol.38 Issue 6, pp.1157-1168

Belge Tipi:

Makale

Yayın Tarihi:

5/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000815981

Sıklık:

İki Aylık

eISSN:

1303-6106

pISSN:

1300-008X