A Modern Approach to Walnut (Juglans regia L.) Sapling Production: Detailed Analysis of Surface Sterilization and Culture Initiation in Tissue Culture

Duygu Özelçi

Makale detayları

A Modern Approach to Walnut (Juglans regia L.) Sapling Production: Detailed Analysis of Surface Sterilization and Culture Initiation in Tissue Culture

Yazarlar:

Duygu Özelçi

Yayıncı:

Siirt University

Ana Başlıklar:

Tarımda Bitki Biyoteknolojisi

Dergi:

Türkiye Tarımsal Araştırmalar Dergisi Vol.11 Issue 1, pp.82-90

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001315073

pISSN:

2148-2306

Anahtar kelimeler:

Özet:

 In this study; the development of an effective surface sterilization for Kozdere, Zengibar and Chandler walnut cultivars and the interaction of different media and 6-benzylaminopurine BAP concentrations in culture initiation were investigated. Axillary buds of walnut cultivars were used as explants. The first stage of tissue culture studies is surface sterilization. Plants with successful surface sterilization can be micropropagated. In this study, walnut materials were surface sterilized by using sodium hypochlorite at doses of 20, 30 and 40 and copper sulphate in addition to sodium hypochlorite. The least contamination 8.16 and darkening 22.45 and the highest bud set 69.39 were observed in Kozdere cultivar treated with 30 sodium hypochlorite and copper sulphate. Murashige and Skoog MS and Driver and Kuniyuki Walnut DKW media were used as culture initiation media. 0.5, 1 and 2 mg L-1 BAP were added to the media. DKW medium was found more successful than MS medium in the propagation of walnut cultivars. The addition of 2 mg L-1 BAP to DKW medium gave the best results for shoot elongation, number of shoots per explant and number of leaves for all cultivars. Among the walnut cultivars, Kozdere cultivar was found statistically significantly different from Zengibar and Chandler cultivars in shoot elongation measurements. As a result, it was revealed that Kozdere cultivar can be used for in vitro propagation of walnut cultivars. It is seen that more comprehensive studies are needed to determine the method for propagation of Zengibar and Chandler cultivars in tissue culture.

 In this study; the development of an effective surface sterilization for Kozdere, Zengibar and Chandler walnut cultivars and the interaction of different media and 6-benzylaminopurine BAP concentrations in culture initiation were investigated. Axillary buds of walnut cultivars were used as explants. The first stage of tissue culture studies is surface sterilization. Plants with successful surface sterilization can be micropropagated. In this study, walnut materials were surface sterilized by using sodium hypochlorite at doses of 20, 30 and 40 and copper sulphate in addition to sodium hypochlorite. The least contamination 8.16 and darkening 22.45 and the highest bud set 69.39 were observed in Kozdere cultivar treated with 30 sodium hypochlorite and copper sulphate. Murashige and Skoog MS and Driver and Kuniyuki Walnut DKW media were used as culture initiation media. 0.5, 1 and 2 mg L-1 BAP were added to the media. DKW medium was found more successful than MS medium in the propagation of walnut cultivars. The addition of 2 mg L-1 BAP to DKW medium gave the best results for shoot elongation, number of shoots per explant and number of leaves for all cultivars. Among the walnut cultivars, Kozdere cultivar was found statistically significantly different from Zengibar and Chandler cultivars in shoot elongation measurements. As a result, it was revealed that Kozdere cultivar can be used for in vitro propagation of walnut cultivars. It is seen that more comprehensive studies are needed to determine the method for propagation of Zengibar and Chandler cultivars in tissue culture.

 In this study; the development of an effective surface sterilization for Kozdere, Zengibar and Chandler walnut cultivars and the interaction of different media and 6-benzylaminopurine BAP concentrations in culture initiation were investigated. Axillary buds of walnut cultivars were used as explants. The first stage of tissue culture studies is surface sterilization. Plants with successful surface sterilization can be micropropagated. In this study, walnut materials were surface sterilized by using sodium hypochlorite at doses of 20, 30 and 40 and copper sulphate in addition to sodium hypochlorite. The least contamination 8.16 and darkening 22.45 and the highest bud set 69.39 were observed in Kozdere cultivar treated with 30 sodium hypochlorite and copper sulphate. Murashige and Skoog MS and Driver and Kuniyuki Walnut DKW media were used as culture initiation media. 0.5, 1 and 2 mg L-1 BAP were added to the media. DKW medium was found more successful than MS medium in the propagation of walnut cultivars. The addition of 2 mg L-1 BAP to DKW medium gave the best results for shoot elongation, number of shoots per explant and number of leaves for all cultivars. Among the walnut cultivars, Kozdere cultivar was found statistically significantly different from Zengibar and Chandler cultivars in shoot elongation measurements. As a result, it was revealed that Kozdere cultivar can be used for in vitro propagation of walnut cultivars. It is seen that more comprehensive studies are needed to determine the method for propagation of Zengibar and Chandler cultivars in tissue culture.

 Bu çalışmada; Kozdere, Zengibar ve Chandler ceviz çeşitleri için etkili bir yüzey sterilizasyonunun geliştirilmesi, kültür başlatmada farklı besi ortamları ve 6-benzilaminopürin BAP konsantrasyonlarının etkileşimi araştırılmıştır. Eksplant olarak ceviz çeşitlerinin aksiller tomurcukları kullanılmıştır. Doku kültürü çalışmalarının ilk aşaması yüzey sterilizasyonudur. Yüzey sterilizasyonu başarılı olan bitkilerin mikroçoğaltımı yapılabilmektedir. Araştırmada, ceviz materyallerine  20, 30 ve 40 dozlarında sodyum hipoklorit ile sodyum hipokloritin yanı sıra bakır sülfat kullanılarak yüzey sterilizasyonu yapılmıştır. Yüzey sterilizasyonunda en az kontaminasyon  8.16 ve kararma  22.45 ile en fazla tomurcuk sürmesi  69.39  30 sodyum hipoklorit ve bakır sülfat uygulamasında Kozdere çeşidinde görülmüştür. Kültür başlatma ortamı olarak Murashige ve Skoog MS ile Driver ve Kuniyuki Walnut DKW besi ortamları kullanılmıştır. Besi ortamlarına 0.5, 1 ve 2 mg L-1 BAP eklenmiştir. Ceviz çeşitlerinin çoğaltılmasında DKW ortamı MS ortamına göre daha başarılı bulunmuştur. DKW ortamına 2 mg L-1 BAP ilavesi tüm çeşitler için sürgün uzaması, eksplant başı sürgün sayısı ve yaprak sayısı için en iyi sonuçları vermiştir. Ceviz çeşitleri arasında sürgün uzaması ölçümlerinde Kozdere çeşidinde, Zengibar ve Chandler çeşitlerine göre istatistiksel olarak anlamlı farklılık bulunmuştur. Sonuç olarak, ceviz çeşitlerinin in vitro çoğaltımında Kozdere çeşidinin kullanılabileceği ortaya konulmuştur. Zengibar ve Chandler çeşitlerinin doku kültüründe çoğaltımında yöntem belirlemek için daha kapsamlı çalışmalara ihtiyaç olduğu görülmektedir.

Makale detayları

A Modern Approach to Walnut (Juglans regia L.) Sapling Production: Detailed Analysis of Surface Sterilization and Culture Initiation in Tissue Culture

Yazarlar:

Duygu Özelçi

Yayıncı:

Siirt University

Ana Başlıklar:

Tarımda Bitki Biyoteknolojisi

Dergi:

Türkiye Tarımsal Araştırmalar Dergisi Vol.11 Issue 1, pp.82-90

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001315073

pISSN:

2148-2306

Micropropagation

in vitro

explant

Zengibar

Kozdere

ChandlerMikroçoğaltım

in vitro

eksplant

Zengibar

Kozdere

Chandler

Sayı

Makale detayları

Yazarlar: Duygu Özelçi

Yayıncı: Siirt University

Ana Başlıklar: Tarımda Bitki Biyoteknolojisi

Dergi: Türkiye Tarımsal Araştırmalar Dergisi Vol.11 Issue 1, pp.82-90

Belge Tipi: Makale

Yayın Tarihi: 3/2024

Belge Dili: Türkçe

Document ID (Org No.): 2001315073

pISSN: 2148-2306

Sayı

Yazarlar:

Duygu Özelçi

Yayıncı:

Siirt University

Ana Başlıklar:

Tarımda Bitki Biyoteknolojisi

Dergi:

Türkiye Tarımsal Araştırmalar Dergisi Vol.11 Issue 1, pp.82-90

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001315073

pISSN:

2148-2306