Investigation Of Muc1 and Muc16 Expressions in Uterus Tissue of Kangal or Kangal Cross-Bred Bitches in Proestrus, Metestrus-Diestrus and Anestrus Periods of Sexual Cycles

Buket Bakır; Hasan Oral; Ebru Karadağ Sarı; Mushap Kuru; Hasan Asker

Makale detayları

Investigation Of Muc1 and Muc16 Expressions in Uterus Tissue of Kangal or Kangal Cross-Bred Bitches in Proestrus, Metestrus-Diestrus and Anestrus Periods of Sexual Cycles

Yazarlar:

Buket Bakır | Hasan Oral | Ebru Karadağ Sarı | Mushap Kuru | Hasan Asker

Yayıncı:

Kafkas University

Ana Başlıklar:

Biyokimya ve Hücre Biyolojisi (Diğer)

Dergi:

Caucasian Journal of Science Vol.10 Issue 1, pp.25-37

Belge Tipi:

Makale

Yayın Tarihi:

10/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293211

pISSN:

2148-6840

Anahtar kelimeler:

Özet:

 Mucins are glycoprotein molecules found in mucus secretion. They have important functions in the reproductive system such as sperm motility, elimination of bacterial damage and facilitating implantation. In this study, MUC1 and MUC16 expressions in uterine tissues of Kangal or Kangal cross-bred bitchs in proestrus, metoestrus-dioestrus and anoestrus sexual periods were investigated by immunohistochemical method. Uterine tissues of 15 healthy and adopted Kangal or Kangal cross-bred bitches aged 3-6 years were used in the study. The biches were divided into 3 groups according to sexual cycle periods as proestrus n5, metaestrus-dioestrus n3 and anaestrus n7. As a result, MUC1 was found to be expressed in both cornu and corpus uteri epithelial cells, crypt epithelial cells, uterine gland cells, stratum vascularis, myometrium and perimetrium layers in canine uterus. When oestrus cycles were compared, it was found that expression increased more in the metoestrus-dioestrus phase compared to other phases. Expression of MUC16 was observed in the cornu uteri during the metoestrus-dioestrus phase. In this study, MUC1 and MUC16 were determined to be expressed in the uterine tissue of bitches and their release was found to be affected by hormones.

 Mucins are glycoprotein molecules found in mucus secretion. They have important functions in the reproductive system such as sperm motility, elimination of bacterial damage and facilitating implantation. In this study, MUC1 and MUC16 expressions in uterine tissues of Kangal or Kangal cross-bred bitchs in proestrus, metoestrus-dioestrus and anoestrus sexual periods were investigated by immunohistochemical method. Uterine tissues of 15 healthy and adopted Kangal or Kangal cross-bred bitches aged 3-6 years were used in the study. The biches were divided into 3 groups according to sexual cycle periods as proestrus n5, metaestrus-dioestrus n3 and anaestrus n7. As a result, MUC1 was found to be expressed in both cornu and corpus uteri epithelial cells, crypt epithelial cells, uterine gland cells, stratum vascularis, myometrium and perimetrium layers in canine uterus. When oestrus cycles were compared, it was found that expression increased more in the metoestrus-dioestrus phase compared to other phases. Expression of MUC16 was observed in the cornu uteri during the metoestrus-dioestrus phase. In this study, MUC1 and MUC16 were determined to be expressed in the uterine tissue of bitches and their release was found to be affected by hormones.

 Mucins are glycoprotein molecules found in mucus secretion. They have important functions in the reproductive system such as sperm motility, elimination of bacterial damage and facilitating implantation. In this study, MUC1 and MUC16 expressions in uterine tissues of Kangal or Kangal cross-bred bitchs in proestrus, metoestrus-dioestrus and anoestrus sexual periods were investigated by immunohistochemical method. Uterine tissues of 15 healthy and adopted Kangal or Kangal cross-bred bitches aged 3-6 years were used in the study. The biches were divided into 3 groups according to sexual cycle periods as proestrus n5, metaestrus-dioestrus n3 and anaestrus n7. As a result, MUC1 was found to be expressed in both cornu and corpus uteri epithelial cells, crypt epithelial cells, uterine gland cells, stratum vascularis, myometrium and perimetrium layers in canine uterus. When oestrus cycles were compared, it was found that expression increased more in the metoestrus-dioestrus phase compared to other phases. Expression of MUC16 was observed in the cornu uteri during the metoestrus-dioestrus phase. In this study, MUC1 and MUC16 were determined to be expressed in the uterine tissue of bitches and their release was found to be affected by hormones.

 Bu çalışmada, seksüel siklus döngülerinin proöstrus, metoöstrus-diöstrus ve anöstrus dönemlerinde Kangal veya Kangal melezi dişi köpeklerin uterus dokusunda MUC1 ve MUC16 ekspresyonları immünohistokimyasal yöntemle incelendi. Çalışmada 3-6 yaş arası sağlıklı ve sahipli Kangal veya Kangal melezi 15 köpeğin uterus dokuları kullanıldı. Köpekler seksüel siklus dönemlerine göre proöstrus n5, metöstrus-diöstrus n3 ve anöstrus n7 olarak 3 gruba ayrıldı. Sonuç olarak, MUC1in köpek uterusunda hem kornu hem de korpus uteri epitel hücrelerinde, kript epitel hücrelerinde, uterus bezi epitel hücrelerinde, stratum vaskülaris, miyometriyum ve perimetriyum tabakalarından eksprese edildiği tespit edildi. Östrus siklusları karşılaştırıldığında metöstrus-diöstrus döneminde ekspresyonun diğer dönemlere göre daha fazla arttığı saptandı. MUC16nın ekspresyonu, metöstrus-diöstrus döneminde kornu uteride gözlendi. Bu çalışmada MUC1 ve MUC16nın köpeklerin uterus dokusundan eksprese edildiği ve salınımlarının hormonlardan etkilendiği tespit edildi.

Makale detayları

Investigation Of Muc1 and Muc16 Expressions in Uterus Tissue of Kangal or Kangal Cross-Bred Bitches in Proestrus, Metestrus-Diestrus and Anestrus Periods of Sexual Cycles

Yazarlar:

Buket Bakır | Hasan Oral | Ebru Karadağ Sarı | Mushap Kuru | Hasan Asker

Yayıncı:

Kafkas University

Ana Başlıklar:

Biyokimya ve Hücre Biyolojisi (Diğer)

Dergi:

Caucasian Journal of Science Vol.10 Issue 1, pp.25-37

Belge Tipi:

Makale

Yayın Tarihi:

10/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293211

pISSN:

2148-6840

bitchs

estrous cycle

mucin

uterusKöpek

Östrus siklusu

Musin

Uterus

Sayı

Makale detayları

Yazarlar: Buket Bakır | Hasan Oral | Ebru Karadağ Sarı | Mushap Kuru | Hasan Asker

Yayıncı: Kafkas University

Ana Başlıklar: Biyokimya ve Hücre Biyolojisi (Diğer)

Dergi: Caucasian Journal of Science Vol.10 Issue 1, pp.25-37

Belge Tipi: Makale

Yayın Tarihi: 10/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001293211

pISSN: 2148-6840

Sayı

Yazarlar:

Buket Bakır | Hasan Oral | Ebru Karadağ Sarı | Mushap Kuru | Hasan Asker

Yayıncı:

Kafkas University

Ana Başlıklar:

Biyokimya ve Hücre Biyolojisi (Diğer)

Dergi:

Caucasian Journal of Science Vol.10 Issue 1, pp.25-37

Belge Tipi:

Makale

Yayın Tarihi:

10/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293211

pISSN:

2148-6840