Cytotoxic Effect of Tricholoma fracticum Extract on Hep3B Cells

Şule İnci; Isik Didem Karagoz; Sevda Kırbağ

Makale detayları

Cytotoxic Effect of Tricholoma fracticum Extract on Hep3B Cells

Yazarlar:

Şule İnci | Isik Didem Karagoz | Sevda Kırbağ

Yayıncı:

Selcuk University

Ana Başlıklar:

Mikoloji

Dergi:

Mantar Dergisi Vol.15 Issue 1, pp.1-6

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001316215

pISSN:

2147-6845

Anahtar kelimeler:

Özet:

 Cancer constitutes one of the greatest threats to human health. Considering that drug resistance is one of the main problems in cancer treatment, studies on the effects of natural compounds on cancer cells are increasing rapidly. Edible mushrooms are considered as a functional and healthy food source because they are rich in nutritional values. Studies have shown that they have anticancer, anti-inflammatory, antioxidant, and antimicrobial effects. Tricholoma fracticum Britzelm. Kreisel, which grows naturally in many regions of our country and in the province of Elazığ, is an edible mushroom consumed as food. In this study, it was aimed to determine the cytotoxic effect of methanol extract of T. fracticum at different concentrations 1000µgmL, 500 µgmL, 250 µgmL, 125 µgmL and 62.5 µgmL against Hep3B cells at 24 and 48 hours. 3-4,5dimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide MTT method was used to determine the cytotoxic effect. In the results obtained, the methanol extract of T. fracticum showed a significant cytotoxic effect at a concentration of 1000 µgmL at the 24th 1.970.65 and 48th hour 0.610.27. The IC50 value of the methanol extract of the fungus against the Hep3B cell line was calculated as 166.767.65 µgmL at 24 hours and 134.385.45 µgmL at 48 hours. We think that the cytotoxic results of T. fracticum extract against Hep3B cell line may be important and this species, which is less studied among Tricholoma species, should be studied more and brought to the literatüre.

 Cancer constitutes one of the greatest threats to human health. Considering that drug resistance is one of the main problems in cancer treatment, studies on the effects of natural compounds on cancer cells are increasing rapidly. Edible mushrooms are considered as a functional and healthy food source because they are rich in nutritional values. Studies have shown that they have anticancer, anti-inflammatory, antioxidant, and antimicrobial effects. Tricholoma fracticum Britzelm. Kreisel, which grows naturally in many regions of our country and in the province of Elazığ, is an edible mushroom consumed as food. In this study, it was aimed to determine the cytotoxic effect of methanol extract of T. fracticum at different concentrations 1000µgmL, 500 µgmL, 250 µgmL, 125 µgmL and 62.5 µgmL against Hep3B cells at 24 and 48 hours. 3-4,5dimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide MTT method was used to determine the cytotoxic effect. In the results obtained, the methanol extract of T. fracticum showed a significant cytotoxic effect at a concentration of 1000 µgmL at the 24th 1.970.65 and 48th hour 0.610.27. The IC50 value of the methanol extract of the fungus against the Hep3B cell line was calculated as 166.767.65 µgmL at 24 hours and 134.385.45 µgmL at 48 hours. We think that the cytotoxic results of T. fracticum extract against Hep3B cell line may be important and this species, which is less studied among Tricholoma species, should be studied more and brought to the literatüre.

 Cancer constitutes one of the greatest threats to human health. Considering that drug resistance is one of the main problems in cancer treatment, studies on the effects of natural compounds on cancer cells are increasing rapidly. Edible mushrooms are considered as a functional and healthy food source because they are rich in nutritional values. Studies have shown that they have anticancer, anti-inflammatory, antioxidant, and antimicrobial effects. Tricholoma fracticum Britzelm. Kreisel, which grows naturally in many regions of our country and in the province of Elazığ, is an edible mushroom consumed as food. In this study, it was aimed to determine the cytotoxic effect of methanol extract of T. fracticum at different concentrations 1000µgmL, 500 µgmL, 250 µgmL, 125 µgmL and 62.5 µgmL against Hep3B cells at 24 and 48 hours. 3-4,5dimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide MTT method was used to determine the cytotoxic effect. In the results obtained, the methanol extract of T. fracticum showed a significant cytotoxic effect at a concentration of 1000 µgmL at the 24th 1.970.65 and 48th hour 0.610.27. The IC50 value of the methanol extract of the fungus against the Hep3B cell line was calculated as 166.767.65 µgmL at 24 hours and 134.385.45 µgmL at 48 hours. We think that the cytotoxic results of T. fracticum extract against Hep3B cell line may be important and this species, which is less studied among Tricholoma species, should be studied more and brought to the literatüre.

 Kanser, insan sağlığı için en büyük tehditlerden birini oluşturmaktadır. İlaç direncinin kanser tedavisindeki temel sorunlardan biri olduğu düşünüldüğünde, doğal bileşiklerin kanser hücreleri üzerindeki etkilerine yönelik çalışmalar hızla artmaktadır. Yenilebilir mantarlar besin değerleri bakımından zengin olmaları nedeniyle fonksiyonel ve sağlıklı gıda kaynağı olarak değerlendirilmektedir. Yapılan çalışmalarda antikanser, anti-inflamatuvar, antioksidan ve antimikrobiyal etkiye sahip oldukları tespit edilmiştir. Ülkemizin birçok bölgesi ve Elazığ ilinde doğal olarak yetişen Tricholoma fracticum Britzelm. Kreisel besin olarak tüketilen yenilebilir bir mantardır. Bu çalışmada T. fracticumun farklı konsantrasyonlardaki 1000µgmL, 500 µgmL, 250 µgmL, 125 µgmL ve 62.5 µgmL metanol ekstresinin 24 ve 48 saatte Hep3B hücrelerine karşı sitotoksik etkisinin belirlenmesi amaçlanmıştır. Sitotoksik etkinin tespit edilmesi için 3-4,5dimetiltiazol-2-iyl-2,5-difenil tetrazolyum bromür MTT yöntemi kullanılmıştır. Elde edilen sonuçlarda T. fracticumun metanol ekstresi 1000 µgmL konsantrasyonda 24. 1.970.65 ve 48. saatte 0.610.27 önemli sitotoksik etki göstermiştir. Mantarın metanol ektresinin Hep3B hücre hattına karşı 24. saatte IC50 değerinin ise 166.767.65 µgmL ve 48. saatte 134.385.45 µgmL olarak hesaplanmıştır. T. fracticum ekstresinin Hep3B hücre hattına karşı elde edilen sitotoksik sonuçlarının önemli olabileceğini ve Tricholoma türleri arasında daha az çalışılan bu türün daha fazla çalışılması gerektiğini ve literatüre kazandırılması gerektiğini düşünmekteyiz.

Makale detayları

Cytotoxic Effect of Tricholoma fracticum Extract on Hep3B Cells

Yazarlar:

Şule İnci | Isik Didem Karagoz | Sevda Kırbağ

Yayıncı:

Selcuk University

Ana Başlıklar:

Mikoloji

Dergi:

Mantar Dergisi Vol.15 Issue 1, pp.1-6

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001316215

pISSN:

2147-6845

Tricholomataceae

Tricholoma fracticum

Medicinal mushroom

Hep3B human hepatoma

Cytotoxic effect

MTTTricholomataceae

Tricholoma fracticum

Tıbbi Mantar

Hep3B insan hepatomu

Sitotoksik etki

MTT

Sayı

Makale detayları

Yazarlar: Şule İnci | Isik Didem Karagoz | Sevda Kırbağ

Yayıncı: Selcuk University

Ana Başlıklar: Mikoloji

Dergi: Mantar Dergisi Vol.15 Issue 1, pp.1-6

Belge Tipi: Makale

Yayın Tarihi: 4/2024

Belge Dili: Türkçe

Document ID (Org No.): 2001316215

pISSN: 2147-6845

Sayı

Yazarlar:

Şule İnci | Isik Didem Karagoz | Sevda Kırbağ

Yayıncı:

Selcuk University

Ana Başlıklar:

Mikoloji

Dergi:

Mantar Dergisi Vol.15 Issue 1, pp.1-6

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001316215

pISSN:

2147-6845