The Relationship Between CACNA2D1 Gene and Subclinic Mastitis in Holstein Breed Cattle

Fadime Daldaban; Korhan Arslan; Bilal Akyüz

Makale detayları

The Relationship Between CACNA2D1 Gene and Subclinic Mastitis in Holstein Breed Cattle

Yazarlar:

Fadime Daldaban | Korhan Arslan | Bilal Akyüz

Yayıncı:

Van Yuzuncu Yıl University

Ana Başlıklar:

Veteriner Bilimleri (Diğer)

Dergi:

Van Veterinary Journal Vol.34 Issue 2, pp.93-97

Belge Tipi:

Makale

Yayın Tarihi:

7/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293484

pISSN:

2149-3359

Anahtar kelimeler:

Özet:

 The CACNA2D1 gene encodes the CACNA2D1 protein and, this protein is involved in the excitation-contraction mechanism of the muscle cells during milk withdrawal, helps the nipples to open and close. Because of this role in physiological mechanism and its relationship with quantitative trait locus QTL regions, CACNA2D1 gene is known to be associated with mastitis resistance. In this study, it was aimed to investigate the relationship between different three SNP C367400T, A496561G and G519663A on the CACNA2D1 gene, and subclinical mastitis in Holstein breed cattle reared in Develi district of Kayseri province. SNPs were genotyped from DNA samples by PCR-RFLP method. In the study, California mastitis test CMT data, and distributions of genotypes of the three SNPs on the CACNA2D1 gene were calculated. In the study, genotype distributions were determined in terms of C367400T, A496561G and G519663A SNPs found on the CACNA2D1 gene according to CMT status. The difference between the C367400T, A496561G and G519663A SNPs was not significant p0.05. In the study group examined the Chi-square χ2 analysis conducted, it was observed that the Holstein cattle were in the Hardy-Weinberg equilibrium HWE in terms of C367400T and A496561G SNPs, deviation from HWE for the G519663A SNP p0.05. As a result, it was thought that the CACNA2D1 gene and these SNPs should be evaluated with more samples and different mastitis indicator data in studies on mastitis resistance.

 The CACNA2D1 gene encodes the CACNA2D1 protein and, this protein is involved in the excitation-contraction mechanism of the muscle cells during milk withdrawal, helps the nipples to open and close. Because of this role in physiological mechanism and its relationship with quantitative trait locus QTL regions, CACNA2D1 gene is known to be associated with mastitis resistance. In this study, it was aimed to investigate the relationship between different three SNP C367400T, A496561G and G519663A on the CACNA2D1 gene, and subclinical mastitis in Holstein breed cattle reared in Develi district of Kayseri province. SNPs were genotyped from DNA samples by PCR-RFLP method. In the study, California mastitis test CMT data, and distributions of genotypes of the three SNPs on the CACNA2D1 gene were calculated. In the study, genotype distributions were determined in terms of C367400T, A496561G and G519663A SNPs found on the CACNA2D1 gene according to CMT status. The difference between the C367400T, A496561G and G519663A SNPs was not significant p0.05. In the study group examined the Chi-square χ2 analysis conducted, it was observed that the Holstein cattle were in the Hardy-Weinberg equilibrium HWE in terms of C367400T and A496561G SNPs, deviation from HWE for the G519663A SNP p0.05. As a result, it was thought that the CACNA2D1 gene and these SNPs should be evaluated with more samples and different mastitis indicator data in studies on mastitis resistance.

 The CACNA2D1 gene encodes the CACNA2D1 protein and, this protein is involved in the excitation-contraction mechanism of the muscle cells during milk withdrawal, helps the nipples to open and close. Because of this role in physiological mechanism and its relationship with quantitative trait locus QTL regions, CACNA2D1 gene is known to be associated with mastitis resistance. In this study, it was aimed to investigate the relationship between different three SNP C367400T, A496561G and G519663A on the CACNA2D1 gene, and subclinical mastitis in Holstein breed cattle reared in Develi district of Kayseri province. SNPs were genotyped from DNA samples by PCR-RFLP method. In the study, California mastitis test CMT data, and distributions of genotypes of the three SNPs on the CACNA2D1 gene were calculated. In the study, genotype distributions were determined in terms of C367400T, A496561G and G519663A SNPs found on the CACNA2D1 gene according to CMT status. The difference between the C367400T, A496561G and G519663A SNPs was not significant p0.05. In the study group examined the Chi-square χ2 analysis conducted, it was observed that the Holstein cattle were in the Hardy-Weinberg equilibrium HWE in terms of C367400T and A496561G SNPs, deviation from HWE for the G519663A SNP p0.05. As a result, it was thought that the CACNA2D1 gene and these SNPs should be evaluated with more samples and different mastitis indicator data in studies on mastitis resistance.

 CACNA2D1 geni, CACNA2D1 proteinini kodlar ve sütün indirilmesi sırasında kas hücrelerinin uyarılma-kasılma mekanizmasında görev alarak meme uçlarının açılıp kapanmasına yardımcı olur. Fizyolojik olarak üstelendiği bu görev ve kantitatif özellik lokusu QTL bölgelerine yakınlığı nedeni ile CACNA2D1 geninin mastitis direnci ile ilişkili olduğu bilinmektedir. Bu çalışmada Kayseri ili Develi ilçesinde yetiştirilen sağmal Holştayn sığırlarda CACNA2D1 geninde bulunan üç farklı SNP bölgesi ile subklinik mastitis durumları arasındaki ilişkinin araştırılması amaçlandı. Çalışma grubunu hepsi üçüncü laktasyonda 151 baş sağmal Holştayn sığır ırkı oluşturdu. Çalışmada California mastitis test CMT durumuna göre CACNA2D1 geni üzerinde bulanan üç SNP C367400T, A496561G ve G519663A yönünden genotip dağılımları belirlendi. Yapılan istatistiki analizler sonucunda C367400T, A496561G ve G519663A SNP genotipleri arasında fark bulunmadı p0.05. Yapılan Chi-Kare analizinde incelenen çalışma grubunda C367400T ve A496561G kodlu SNPler yönünden Hardy-Weinberg dengesinde HWE oldukları, G519663A kodlu SNP yönünden ise HWEden saptıkları P0.05 gözlemlendi. Sonuç olarak mastitis direnci ile ilgili çalışmalarda CACNA2D1 geni ve bu SNPlerin daha fazla örneklemle ve farklı mastitis göstergesi verilerle değerlendirilmesi gerektiği düşünüldü.

Makale detayları

The Relationship Between CACNA2D1 Gene and Subclinic Mastitis in Holstein Breed Cattle

Yazarlar:

Fadime Daldaban | Korhan Arslan | Bilal Akyüz

Yayıncı:

Van Yuzuncu Yıl University

Ana Başlıklar:

Veteriner Bilimleri (Diğer)

Dergi:

Van Veterinary Journal Vol.34 Issue 2, pp.93-97

Belge Tipi:

Makale

Yayın Tarihi:

7/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293484

pISSN:

2149-3359

CACNA2D1

Bovine mastitis

Cattle

Genetic markerCACNA2D1

Genetik belirteç

Sığır

Sığır mastit

Sayı

Makale detayları

Yazarlar: Fadime Daldaban | Korhan Arslan | Bilal Akyüz

Yayıncı: Van Yuzuncu Yıl University

Ana Başlıklar: Veteriner Bilimleri (Diğer)

Dergi: Van Veterinary Journal Vol.34 Issue 2, pp.93-97

Belge Tipi: Makale

Yayın Tarihi: 7/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001293484

pISSN: 2149-3359

Sayı

Yazarlar:

Fadime Daldaban | Korhan Arslan | Bilal Akyüz

Yayıncı:

Van Yuzuncu Yıl University

Ana Başlıklar:

Veteriner Bilimleri (Diğer)

Dergi:

Van Veterinary Journal Vol.34 Issue 2, pp.93-97

Belge Tipi:

Makale

Yayın Tarihi:

7/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293484

pISSN:

2149-3359