Comparative evaluation of factors affecting hemagglutinatingactivity of avian influenza (H9) virus

Asfa RASOOL; Sameera AKHTAR; Khushi MUHAMMAD; Masood RABBANI; Aftab Ahmed ANJUM; Javed MUHAMMAD; Arfan AHMAD; Ali Ahmad SHEIKH; Fakhra LIAQAT; Fariha AKHTAR; Rabia TAHIR

Makale detayları

Comparative evaluation of factors affecting hemagglutinatingactivity of avian influenza (H9) virus

Yazarlar:

Asfa RASOOL | Sameera AKHTAR | Khushi MUHAMMAD | Masood RABBANI | Aftab Ahmed ANJUM | Javed MUHAMMAD | Arfan AHMAD | Ali Ahmad SHEIKH | Fakhra LIAQAT | Fariha AKHTAR | Rabia TAHIR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2016 Vol.40 Issue 1, pp.102-106

Belge Tipi:

Makale

Yayın Tarihi:

1/2016

Belge Dili:

Türkçe

Document ID (Org No.):

2000765318

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Özet:

 

Influenza virus H9 was standardized for hemagglutination assay using different factors such as red blood cell (RBC) types, concentrations, diluent types, and storage times. Avian influenza virus H9 was grown in embryonated chicken eggs and confirmed by spot agglutination. A significant (P  0.05) were found between human blood type O, chicken, and dog RBCs, as well as among rabbit, pigeon, sheep, and parrot. The highest titer (9.00 ± 0.00) with a nonsignificant difference was found using virus stored at 4 °C and -20 °C while 37 °C showed the lowest significant mean hemagglutinin (HA) titer (11.08 ± 188.21). Nonsignificant differences were observed in HA titers against H9 virus stored for 3, 4, 5, and 6 months. Nonsignificant differences were found between the use of normal saline and 0.5% peptone water with the lowest HA titers of 7.83 ± 0.40 and 8.00 ± 0.00, respectively, while the highest HA titer (9.00 ± 0.00) with nonsignificant difference was observed by using HA-HI buffer and phosphate buffer saline as diluents. RBCs with 0.5% and 1% concentrations showed nonsignificant difference in HA titer but significant difference with 0.1% RBCs.

Makale detayları

Comparative evaluation of factors affecting hemagglutinatingactivity of avian influenza (H9) virus

Yazarlar: Asfa RASOOL | Sameera AKHTAR | Khushi MUHAMMAD | Masood RABBANI | Aftab Ahmed ANJUM | Javed MUHAMMAD | Arfan AHMAD | Ali Ahmad SHEIKH | Fakhra LIAQAT | Fariha AKHTAR | Rabia TAHIR

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2016 Vol.40 Issue 1, pp.102-106

Belge Tipi: Makale

Yayın Tarihi: 1/2016

Belge Dili: Türkçe

Document ID (Org No.): 2000765318

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Sayı

Yazarlar:

Asfa RASOOL | Sameera AKHTAR | Khushi MUHAMMAD | Masood RABBANI | Aftab Ahmed ANJUM | Javed MUHAMMAD | Arfan AHMAD | Ali Ahmad SHEIKH | Fakhra LIAQAT | Fariha AKHTAR | Rabia TAHIR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2016 Vol.40 Issue 1, pp.102-106

Belge Tipi:

Makale

Yayın Tarihi:

1/2016

Belge Dili:

Türkçe

Document ID (Org No.):

2000765318

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128