Morphological and molecular identification of Dissingia confusa based on the first record of the species in Türkiye

Şuheda Sümeyye Terman; Ayten Dizkırıcı; Mustafa Emre Akçay; Cemil Sadullahoğlu

Makale detayları

Morphological and molecular identification of Dissingia confusa based on the first record of the species in Türkiye

Yazarlar:

Şuheda Sümeyye Terman | Ayten Dizkırıcı | Mustafa Emre Akçay | Cemil Sadullahoğlu

Yayıncı:

Trakya University

Ana Başlıklar:

Plant and Fungus Systematics and Taxonomy

Dergi:

Trakya University Journal of Natural Sciences Vol.25 Issue 1, pp.65-72

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001281122

pISSN:

2528-9691

Anahtar kelimeler:

Özet:

 In the present study, specimens kept in Fungarium of Van Yüzüncü Yıl University - Türkiye VANF were evaluated based on morphological and molecular characters, and identified as Dissingia confusa as a new record from Eastern Türkiye. Ascocarp, hymenium, stipe, asco-spores, ascus and paraphyses were considered as diagnostic morphological characters and the nucleotide sequences of the large subunit LSU ribosomal DNA and the translation elonga-tion factor 1-alpha TEF1-α were referred as molecular data. A phylogenetic analysis was performed with Maximum parsimony and Bayesian inference methods. The DNA sequences of 15 7 for TEF1-α Dissingia and 27 14 for TEF1-α Helvella specimens downloaded from the GenBank database were included in the analysis to estimate phylogeny between the two close genera. Morphological evaluations and genetic evidences confirmed that Dissingia is phylogenetically separated from Helvella at genus level and helped to identify the studied specimens as D. confusa as a new record in Türkiye. Detailed description, colour images of fresh and dried ascomata, along with photographs of microscopic characters and the obtained phylogenetic trees are given.

 In the present study, specimens kept in Fungarium of Van Yüzüncü Yıl University - Türkiye VANF were evaluated based on morphological and molecular characters, and identified as Dissingia confusa as a new record from Eastern Türkiye. Ascocarp, hymenium, stipe, asco-spores, ascus and paraphyses were considered as diagnostic morphological characters and the nucleotide sequences of the large subunit LSU ribosomal DNA and the translation elonga-tion factor 1-alpha TEF1-α were referred as molecular data. A phylogenetic analysis was performed with Maximum parsimony and Bayesian inference methods. The DNA sequences of 15 7 for TEF1-α Dissingia and 27 14 for TEF1-α Helvella specimens downloaded from the GenBank database were included in the analysis to estimate phylogeny between the two close genera. Morphological evaluations and genetic evidences confirmed that Dissingia is phylogenetically separated from Helvella at genus level and helped to identify the studied specimens as D. confusa as a new record in Türkiye. Detailed description, colour images of fresh and dried ascomata, along with photographs of microscopic characters and the obtained phylogenetic trees are given.

 In the present study, specimens kept in Fungarium of Van Yüzüncü Yıl University - Türkiye VANF were evaluated based on morphological and molecular characters, and identified as Dissingia confusa as a new record from Eastern Türkiye. Ascocarp, hymenium, stipe, asco-spores, ascus and paraphyses were considered as diagnostic morphological characters and the nucleotide sequences of the large subunit LSU ribosomal DNA and the translation elonga-tion factor 1-alpha TEF1-α were referred as molecular data. A phylogenetic analysis was performed with Maximum parsimony and Bayesian inference methods. The DNA sequences of 15 7 for TEF1-α Dissingia and 27 14 for TEF1-α Helvella specimens downloaded from the GenBank database were included in the analysis to estimate phylogeny between the two close genera. Morphological evaluations and genetic evidences confirmed that Dissingia is phylogenetically separated from Helvella at genus level and helped to identify the studied specimens as D. confusa as a new record in Türkiye. Detailed description, colour images of fresh and dried ascomata, along with photographs of microscopic characters and the obtained phylogenetic trees are given.

 Van Yüzüncü Yıl Üniversitesi Fungaryumunda VANF muhafaza edilen Dissingia confusa türü morfolojik ve moleküler karakterler ile Türkiyenin doğusundan yeni bir kayıt olarak tanımlanmıştır. Şapka, himenyum, sap, spor, askus ve parafiz yapıları morfolojik veri olarak, büyük alt ünite LSU ve translasyon uzama faktörü 1-alfa TEF1-α bölgelerinin nükleotid dizileri moleküler veri olarak kullanılmıştır. Filogenetik analiz Maksimum parsimoni ve Bayesian çıkarım yöntemi ile gerçekleştirilmiştir. Analizlere, iki cins arasındaki filogeniyi göstermek için GenBank veritabanından indirilen 15 TEF1-α için 7 Dissingia ve 27 TEF1-α için 14 Helvella örnekleri de eklenmiştir. Morfolojik çalışmalar ve genetik kanıtlar, Dissingia cinsinin cins düzeyinde Helvella cinsinden filogenetik olarak ayrıldığını ve Dissingia confusa türünün Türkiyede ilk kez tanımlandığını doğrulamıştır. Detaylı deskripsiyon, taze ve kuru örneklerin renkli fotoğrafları ile mikroskobik karakterlerin görüntüleri ve filogenetik ağaçlar verilmiştir.

Makale detayları

Morphological and molecular identification of Dissingia confusa based on the first record of the species in Türkiye

Yazarlar:

Şuheda Sümeyye Terman | Ayten Dizkırıcı | Mustafa Emre Akçay | Cemil Sadullahoğlu

Yayıncı:

Trakya University

Ana Başlıklar:

Plant and Fungus Systematics and Taxonomy

Dergi:

Trakya University Journal of Natural Sciences Vol.25 Issue 1, pp.65-72

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001281122

pISSN:

2528-9691

Dissingia

Helvella

molecular taxonomy

new record

phylogeny

Sayı

Makale detayları

Morphological and molecular identification of Dissingia confusa based on the first record of the species in Türkiye

Yazarlar: Şuheda Sümeyye Terman | Ayten Dizkırıcı | Mustafa Emre Akçay | Cemil Sadullahoğlu

Yayıncı: Trakya University

Ana Başlıklar: Plant and Fungus Systematics and Taxonomy

Dergi: Trakya University Journal of Natural Sciences Vol.25 Issue 1, pp.65-72

Belge Tipi: Makale

Yayın Tarihi: 4/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001281122

pISSN: 2528-9691

Dissingia

Helvella

molecular taxonomy

new record

phylogeny

Sayı

Yazarlar:

Şuheda Sümeyye Terman | Ayten Dizkırıcı | Mustafa Emre Akçay | Cemil Sadullahoğlu

Yayıncı:

Trakya University

Ana Başlıklar:

Plant and Fungus Systematics and Taxonomy

Dergi:

Trakya University Journal of Natural Sciences Vol.25 Issue 1, pp.65-72

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001281122

pISSN:

2528-9691