An Indirect Lectin Affinity Bioassay for Uromodulin

Zeki TOPCU

Makale detayları

An Indirect Lectin Affinity Bioassay for Uromodulin

Yazarlar:

Zeki TOPCU

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Chemistry 2002 Vol.26 Issue 3, pp.363-368

Belge Tipi:

Makale

Yayın Tarihi:

2/2002

Belge Dili:

Türkçe

Document ID (Org No.):

2000729890

Sıklık:

İki Aylık

eISSN:

1303-6130

pISSN:

1300-0527

Özet:

 

Uromodulin is a monomeric glycoprotein excreted in large amounts in urine. Most of the assay methods for quantifying  uromodulin rely on the   availability of antibodies to this glycoprotein. This study reports a   new bioassay for uromodulin without requiring the presence of    antibodies. The bioassay   is based on the lectin affinity of the glycoprotein and the known interaction   between uromodulin and the tumor necrosis factor. The measuring range of the   bioassay described in this paper is between 0.13 and 1.25 m g/ml, which   makes it a potential method for clinical use.

 

Uromodulin is a monomeric glycoprotein excreted in large amounts in urine. Most of the assay methods for quantifying  uromodulin rely on the   availability of antibodies to this glycoprotein. This study reports a   new bioassay for uromodulin without requiring the presence of    antibodies. The bioassay   is based on the lectin affinity of the glycoprotein and the known interaction   between uromodulin and the tumor necrosis factor. The measuring range of the   bioassay described in this paper is between 0.13 and 1.25 m g/ml, which   makes it a potential method for clinical use.

Makale detayları

An Indirect Lectin Affinity Bioassay for Uromodulin

Yazarlar: Zeki TOPCU

Yayıncı: TUBITAK

Dergi: Turkish Journal of Chemistry 2002 Vol.26 Issue 3, pp.363-368

Belge Tipi: Makale

Yayın Tarihi: 2/2002

Belge Dili: Türkçe

Document ID (Org No.): 2000729890

Sıklık: İki Aylık

eISSN: 1303-6130

pISSN: 1300-0527

Sayı

Yazarlar:

Zeki TOPCU

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Chemistry 2002 Vol.26 Issue 3, pp.363-368

Belge Tipi:

Makale

Yayın Tarihi:

2/2002

Belge Dili:

Türkçe

Document ID (Org No.):

2000729890

Sıklık:

İki Aylık

eISSN:

1303-6130

pISSN:

1300-0527