Role of mesenchymal stem cell-derived soluble factors and folic acid in wound healing

Ahu PAKDEMİRLİ; Feriha TOKSÖZ; Aslihan KARADAĞ; Hüseyin Koray MISIRLIOĞLU; Yasemin BAŞBINAR; Hülya ELLİDOKUZ; Osman AÇIKGÖZ

Makale detayları

Role of mesenchymal stem cell-derived soluble factors and folic acid in wound healing

Yazarlar:

Ahu PAKDEMİRLİ | Feriha TOKSÖZ | Aslihan KARADAĞ | Hüseyin Koray MISIRLIOĞLU | Yasemin BAŞBINAR | Hülya ELLİDOKUZ | Osman AÇIKGÖZ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2019 Vol.49 Issue 3, pp.914-921

Belge Tipi:

Makale

Yayın Tarihi:

5/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000750136

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Özet:

 

Background/aim: Mesenchymal stem cells (MSCs) are a type of adult stem cell consisting of a heterogeneous subset of stromal stem cells that can be isolated from adult tissues. Folic acid is another important contributor to tissue regeneration and repair, which affects the  synthesis  of  some  building  block  molecules  used  for  wound  healing.  In  this  study,  we  examine  the  effect  of  folic  acid  and  MSC-derived soluble factors in the wound healing model.Materials and methods: Human umbilical vein endothelial cells (HUVECs) and bone marrow-derived MSCs (BMSCs) were cultured for  this  study.  Cell  proliferation  analysis  was  done  with  xCELLigence  RTCA.  After  48  h  of  cultivation,  the  cell  culture  medium  was  collected as MSC conditional medium containing mesenchymal stem cell-derived soluble factors (MDFs). Different concentrations of MDFs (12%, 25%, 50%, 75%, and 100%) were applied to the HUVEC cell line. Folic acid (25, 30, 50, 60, 75, 90, and 100 μM) was tested by application of three different groups (control, 25 μM folic acid, 625 μM folic acid inhibitors) for proliferation on the HUVEC cell line. The combined effects of folic acid and MDFs were tested on the HUVEC cell line with 25 μM folic acid and 50 μM MDFs. All data were statistically analyzed using SPSS 15.0 for Windows.Results: Significant differences were observed between controls and cells treated with folic acid, as well as between controls and both folic acid and MDFs (P < 0.05). Among the treated groups, the fastest wound closure rate was seen in cells treated with both folic acid and MDFs.Conclusion: The results show that both folic acid and MDFs increased the wound healing rate in HUVECs when they were used sepa-rately. The strongest benefits were seen in treatment using folic acid and MDFs together.

Makale detayları

Role of mesenchymal stem cell-derived soluble factors and folic acid in wound healing

Yazarlar: Ahu PAKDEMİRLİ | Feriha TOKSÖZ | Aslihan KARADAĞ | Hüseyin Koray MISIRLIOĞLU | Yasemin BAŞBINAR | Hülya ELLİDOKUZ | Osman AÇIKGÖZ

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2019 Vol.49 Issue 3, pp.914-921

Belge Tipi: Makale

Yayın Tarihi: 5/2019

Belge Dili: İngilizce

Document ID (Org No.): 2000750136

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Sayı

Yazarlar:

Ahu PAKDEMİRLİ | Feriha TOKSÖZ | Aslihan KARADAĞ | Hüseyin Koray MISIRLIOĞLU | Yasemin BAŞBINAR | Hülya ELLİDOKUZ | Osman AÇIKGÖZ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2019 Vol.49 Issue 3, pp.914-921

Belge Tipi:

Makale

Yayın Tarihi:

5/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000750136

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144