Production of Double Haploid Plants Using In Vivo Haploid Techniques in Corn

İlhan TURGUT; 2.	duran ZARARSIZ; 3.	semra YANIKOĞLU; 4.	leyla ÖZTÜR  AKAR; 5.	sezgin KIZIK; 6.	burak BİLGİN

doi:https://doi.org/10.15832/ankutbd.539000

Makale detayları

Production of Double Haploid Plants Using In Vivo Haploid Techniques in Corn

Yazarlar:

İlhan TURGUT | 2. duran ZARARSIZ | 3. semra YANIKOĞLU | 4. leyla ÖZTÜR AKAR | 5. sezgin KIZIK | 6. burak BİLGİN

Yayıncı:

Ankara University

Ana Başlıklar:

Uygulamalı fizik

Dergi:

Journal of Agricultural Sciences 2019 Vol.25 Issue 1, pp.62-69

Belge Tipi:

Makale

Yayın Tarihi:

3/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000607430

Sıklık:

Üç Aylık

eISSN:

2148-9297

DOI:

https://doi.org/10.15832/ankutbd.539000

Özet:

 


This research was conducted at the breeding station of the Turkish breeding company Agromar A.Ş in the city of Bursa in Turkey during the 2013 and 2014 growing seasons. Used from within the same heterotic group crossings, 7 donor materials were obtained during the 2012 winter season in the greenhouse. The inducer line RWK-76xRWS, provided by University of Hohenheim, Germany, was used for generating haploid seeds. The donor and inducer crossing was performed during the 2013 summer season. The haploid selection and chromosome doubling were performed during the 2014 summer season. Seven donors were used for haploid induction which name are DNR1, DNR2, DNR3, DNR4, DNR5, DNR6, DNR7 respectively, from each donor different amount of ear crosses were performed (DNR1:16 ears, DNR2:10 ears, DNR3:10 ears, DNR4:12 ears, DNR5:11 ears, DNR6:13 ears, DNR7:11 ears). According to the present study, the average induction rate found ranged from 7.1 to 12.8%, and the average seedling survival rate in the greenhouse after colchicine application ranged from 57.9 to 77.6%. After transplanting to the field, 78.3-92.6% of these plants survived. As a result of this research, the chromosome doubling rate ranged from 22.5 to 48.3% depending on the donor material. These result indicates that maternal haploid selection visually is easy. Haploid induction rate (HIR) changes from donor to donor, its mean genotype and environment is effective for HIR. Average chromosome doubling rate is lower than other researchers’ results, it is also effected by genotype and chromosome doubling methods. Zea mays; Haploid line; Double haploid line; In vivo induction

.

Makale detayları

Production of Double Haploid Plants Using In Vivo Haploid Techniques in Corn

Yazarlar: İlhan TURGUT | 2. duran ZARARSIZ | 3. semra YANIKOĞLU | 4. leyla ÖZTÜR AKAR | 5. sezgin KIZIK | 6. burak BİLGİN

Yayıncı: Ankara University

Ana Başlıklar: Uygulamalı fizik

Dergi: Journal of Agricultural Sciences 2019 Vol.25 Issue 1, pp.62-69

Belge Tipi: Makale

Yayın Tarihi: 3/2019

Belge Dili: İngilizce

Document ID (Org No.): 2000607430

Sıklık: Üç Aylık

eISSN: 2148-9297

DOI: https://doi.org/10.15832/ankutbd.539000

Sayı

Yazarlar:

İlhan TURGUT | 2. duran ZARARSIZ | 3. semra YANIKOĞLU | 4. leyla ÖZTÜR AKAR | 5. sezgin KIZIK | 6. burak BİLGİN

Yayıncı:

Ankara University

Ana Başlıklar:

Uygulamalı fizik

Dergi:

Journal of Agricultural Sciences 2019 Vol.25 Issue 1, pp.62-69

Belge Tipi:

Makale

Yayın Tarihi:

3/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000607430

Sıklık:

Üç Aylık

eISSN:

2148-9297

DOI:

https://doi.org/10.15832/ankutbd.539000