Investigation of the Release of Growth Factors from  Apheresis Platelet Concentrate (APC) Loaded Three  Layered Composite Nanofiber Surface

Hülya Yılmaz; Cansu Aras; Mehmet Karaçay; Merve İlkay Altuntuğ Cesur; Esra Karaca; Şehime G Temel; Emel Başkan; Barbaros Oral; Ekrem Kaya

Makale detayları

Investigation of the Release of Growth Factors from Apheresis Platelet Concentrate (APC) Loaded Three Layered Composite Nanofiber Surface

Yazarlar:

Hülya Yılmaz | Cansu Aras | Mehmet Karaçay | Merve İlkay Altuntuğ Cesur | Esra Karaca | Şehime G Temel | Emel Başkan | Barbaros Oral | Ekrem Kaya

Yayıncı:

Ege University

Ana Başlıklar:

Composite and Hybrid Materials

Dergi:

Textile and Apparel Vol.34 Issue 3, pp.244-252

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001328754

pISSN:

1300-3356

Anahtar kelimeler:

Özet:

 In this study, a nanofiber surface loaded with apheresis platelet concentrate APC was produced for the first time to develop a bioactive wound dressing design. Nanofiber surface n5 consisting of polyurethane polymer outer layer, polyvinyl alcohol polymer middle layer, and polycaprolactone polymer matrix inner layer were produced via the electrospinning method. The surface morphologies of the produced nanofiber surfaces were examined by scanning electron microscopy. Quantitative analyzes of growth factors released from the APC-loaded composite nanofiber surfaces into phosphate-buffered saline at certain time intervals were performed with the ELISA. When the release amounts between bFGF, EGF, and PDGF-AA groups were compared, a significant difference was found in all periods p0.05. When the time-dependent release changes of each group were examined, there was no statistically significant difference in the bFGF group p0.05, but there was a significant difference between the EGF and PDGF-AA growth factors p0.05.

 In this study, a nanofiber surface loaded with apheresis platelet concentrate APC was produced for the first time to develop a bioactive wound dressing design. Nanofiber surface n5 consisting of polyurethane polymer outer layer, polyvinyl alcohol polymer middle layer, and polycaprolactone polymer matrix inner layer were produced via the electrospinning method. The surface morphologies of the produced nanofiber surfaces were examined by scanning electron microscopy. Quantitative analyzes of growth factors released from the APC-loaded composite nanofiber surfaces into phosphate-buffered saline at certain time intervals were performed with the ELISA. When the release amounts between bFGF, EGF, and PDGF-AA groups were compared, a significant difference was found in all periods p0.05. When the time-dependent release changes of each group were examined, there was no statistically significant difference in the bFGF group p0.05, but there was a significant difference between the EGF and PDGF-AA growth factors p0.05.

 In this study, a nanofiber surface loaded with apheresis platelet concentrate APC was produced for the first time to develop a bioactive wound dressing design. Nanofiber surface n5 consisting of polyurethane polymer outer layer, polyvinyl alcohol polymer middle layer, and polycaprolactone polymer matrix inner layer were produced via the electrospinning method. The surface morphologies of the produced nanofiber surfaces were examined by scanning electron microscopy. Quantitative analyzes of growth factors released from the APC-loaded composite nanofiber surfaces into phosphate-buffered saline at certain time intervals were performed with the ELISA. When the release amounts between bFGF, EGF, and PDGF-AA groups were compared, a significant difference was found in all periods p0.05. When the time-dependent release changes of each group were examined, there was no statistically significant difference in the bFGF group p0.05, but there was a significant difference between the EGF and PDGF-AA growth factors p0.05.

 In this study, a nanofiber surface loaded with apheresis platelet concentrate APC was produced for the first time to develop a bioactive wound dressing design. Nanofiber surface n5 consisting of polyurethane polymer outer layer, polyvinyl alcohol polymer middle layer, and polycaprolactone polymer matrix inner layer were produced via the electrospinning method. The surface morphologies of the produced nanofiber surfaces were examined by scanning electron microscopy. Quantitative analyzes of growth factors released from the APC-loaded composite nanofiber surfaces into phosphate-buffered saline at certain time intervals were performed with the ELISA. When the release amounts between bFGF, EGF, and PDGF-AA groups were compared, a significant difference was found in all periods p0.05. When the time-dependent release changes of each group were examined, there was no statistically significant difference in the bFGF group p0.05, but there was a significant difference between the EGF and PDGF-AA growth factors p0.05.

Makale detayları

Investigation of the Release of Growth Factors from Apheresis Platelet Concentrate (APC) Loaded Three Layered Composite Nanofiber Surface

Yazarlar:

Hülya Yılmaz | Cansu Aras | Mehmet Karaçay | Merve İlkay Altuntuğ Cesur | Esra Karaca | Şehime G Temel | Emel Başkan | Barbaros Oral | Ekrem Kaya

Yayıncı:

Ege University

Ana Başlıklar:

Composite and Hybrid Materials

Dergi:

Textile and Apparel Vol.34 Issue 3, pp.244-252

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001328754

pISSN:

1300-3356

Release of growth factors

nanofiber

electrospinning

apheresis platelet concentrate

Sayı

Makale detayları

Investigation of the Release of Growth Factors from Apheresis Platelet Concentrate (APC) Loaded Three Layered Composite Nanofiber Surface

Yazarlar: Hülya Yılmaz | Cansu Aras | Mehmet Karaçay | Merve İlkay Altuntuğ Cesur | Esra Karaca | Şehime G Temel | Emel Başkan | Barbaros Oral | Ekrem Kaya

Yayıncı: Ege University

Ana Başlıklar: Composite and Hybrid Materials

Dergi: Textile and Apparel Vol.34 Issue 3, pp.244-252

Belge Tipi: Makale

Yayın Tarihi: 9/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001328754

pISSN: 1300-3356

Release of growth factors

nanofiber

electrospinning

apheresis platelet concentrate

Sayı

Yazarlar:

Hülya Yılmaz | Cansu Aras | Mehmet Karaçay | Merve İlkay Altuntuğ Cesur | Esra Karaca | Şehime G Temel | Emel Başkan | Barbaros Oral | Ekrem Kaya

Yayıncı:

Ege University

Ana Başlıklar:

Composite and Hybrid Materials

Dergi:

Textile and Apparel Vol.34 Issue 3, pp.244-252

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001328754

pISSN:

1300-3356