Bioactivity of secondary metabolite of endophytic fungi extract isolated from root of Jambu Mawar (Syzygium jambos (L.) Alston)

Kurratul Aini; Elfita Elfita

Makale detayları

Bioactivity of secondary metabolite of endophytic fungi extract isolated from root of Jambu Mawar (Syzygium jambos (L.) Alston)

Yazarlar:

Kurratul Aini | Elfita Elfita

Yayıncı:

İzzet KARA

Ana Başlıklar:

Microbiology (Other)

Dergi:

International Journal of Secondary Metabolite Vol.12 Issue 1, pp.16-32

Belge Tipi:

Makale

Yayın Tarihi:

2/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001331127

pISSN:

2148-6905

Anahtar kelimeler:

Özet:

 This research aims to isolate endophytic fungi from Syzygium jambos and identify their active compounds. Endophytic fungi were isolated from the roots of S. jambos and cultured on Potato Dextrose Agar media. Antibacterial activity using the KirbyBauer method was tested on four Gram-positive and Gram-negative bacteria. Molecular identification was carried out on selected isolates to determine the species of endophytic fungi and isolate their active compounds. Column chromatography was used for compound isolation. The pure compounds were then analyzed spectroscopically using 1H-NMR, 13C-NMR, DEPT 135, HMQC, HMBC, COSY. The results of the isolation of endophytic fungi found four isolates SJR1  4, which show antibacterial activity. The strongest antibacterial activity was demonstrated by isolate SJR1, so it was continued with molecular identification. Molecular identification of SJR1 indicates that it is Lasiodiplodia iranensis. The pure compound L. iranensis was isolated and found to be 3-butyl-3,4-dihydroxy-6-2-hydroxy-5-oxocyclopentyl methyltetrahydro-pyran-2-one, which belongs to the phenolic group and has potential as an antibacterial. This compound can be used as an alternative medicinal ingredient.

 This research aims to isolate endophytic fungi from Syzygium jambos and identify their active compounds. Endophytic fungi were isolated from the roots of S. jambos and cultured on Potato Dextrose Agar media. Antibacterial activity using the KirbyBauer method was tested on four Gram-positive and Gram-negative bacteria. Molecular identification was carried out on selected isolates to determine the species of endophytic fungi and isolate their active compounds. Column chromatography was used for compound isolation. The pure compounds were then analyzed spectroscopically using 1H-NMR, 13C-NMR, DEPT 135, HMQC, HMBC, COSY. The results of the isolation of endophytic fungi found four isolates SJR1  4, which show antibacterial activity. The strongest antibacterial activity was demonstrated by isolate SJR1, so it was continued with molecular identification. Molecular identification of SJR1 indicates that it is Lasiodiplodia iranensis. The pure compound L. iranensis was isolated and found to be 3-butyl-3,4-dihydroxy-6-2-hydroxy-5-oxocyclopentyl methyltetrahydro-pyran-2-one, which belongs to the phenolic group and has potential as an antibacterial. This compound can be used as an alternative medicinal ingredient.

 This research aims to isolate endophytic fungi from Syzygium jambos and identify their active compounds. Endophytic fungi were isolated from the roots of S. jambos and cultured on Potato Dextrose Agar media. Antibacterial activity using the KirbyBauer method was tested on four Gram-positive and Gram-negative bacteria. Molecular identification was carried out on selected isolates to determine the species of endophytic fungi and isolate their active compounds. Column chromatography was used for compound isolation. The pure compounds were then analyzed spectroscopically using 1H-NMR, 13C-NMR, DEPT 135, HMQC, HMBC, COSY. The results of the isolation of endophytic fungi found four isolates SJR1  4, which show antibacterial activity. The strongest antibacterial activity was demonstrated by isolate SJR1, so it was continued with molecular identification. Molecular identification of SJR1 indicates that it is Lasiodiplodia iranensis. The pure compound L. iranensis was isolated and found to be 3-butyl-3,4-dihydroxy-6-2-hydroxy-5-oxocyclopentyl methyltetrahydro-pyran-2-one, which belongs to the phenolic group and has potential as an antibacterial. This compound can be used as an alternative medicinal ingredient.

 This research aims to isolate endophytic fungi from Syzygium jambos and identify their active compounds. Endophytic fungi were isolated from the roots of S. jambos and cultured on Potato Dextrose Agar media. Antibacterial activity using the KirbyBauer method was tested on four Gram-positive and Gram-negative bacteria. Molecular identification was carried out on selected isolates to determine the species of endophytic fungi and isolate their active compounds. Column chromatography was used for compound isolation. The pure compounds were then analyzed spectroscopically using 1H-NMR, 13C-NMR, DEPT 135, HMQC, HMBC, COSY. The results of the isolation of endophytic fungi found four isolates SJR1  4, which show antibacterial activity. The strongest antibacterial activity was demonstrated by isolate SJR1, so it was continued with molecular identification. Molecular identification of SJR1 indicates that it is Lasiodiplodia iranensis. The pure compound L. iranensis was isolated and found to be 3-butyl-3,4-dihydroxy-6-2-hydroxy-5-oxocyclopentyl methyltetrahydro-pyran-2-one, which belongs to the phenolic group and has potential as an antibacterial. This compound can be used as an alternative medicinal ingredient.

Makale detayları

Bioactivity of secondary metabolite of endophytic fungi extract isolated from root of Jambu Mawar (Syzygium jambos (L.) Alston)

Yazarlar:

Kurratul Aini | Elfita Elfita

Yayıncı:

İzzet KARA

Ana Başlıklar:

Microbiology (Other)

Dergi:

International Journal of Secondary Metabolite Vol.12 Issue 1, pp.16-32

Belge Tipi:

Makale

Yayın Tarihi:

2/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001331127

pISSN:

2148-6905

Fungal endophyte

Lasiodiplodia iranensis

Secondary metabolitesFungal endophyte

Lasiodiplodia iranensis

Secondary metabolites

Sayı

Makale detayları

Bioactivity of secondary metabolite of endophytic fungi extract isolated from root of Jambu Mawar (Syzygium jambos (L.) Alston)

Yazarlar: Kurratul Aini | Elfita Elfita

Yayıncı: İzzet KARA

Ana Başlıklar: Microbiology (Other)

Dergi: International Journal of Secondary Metabolite Vol.12 Issue 1, pp.16-32

Belge Tipi: Makale

Yayın Tarihi: 2/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001331127

pISSN: 2148-6905

Fungal endophyte

Lasiodiplodia iranensis

Secondary metabolitesFungal endophyte

Lasiodiplodia iranensis

Secondary metabolites

Sayı

Yazarlar:

Kurratul Aini | Elfita Elfita

Yayıncı:

İzzet KARA

Ana Başlıklar:

Microbiology (Other)

Dergi:

International Journal of Secondary Metabolite Vol.12 Issue 1, pp.16-32

Belge Tipi:

Makale

Yayın Tarihi:

2/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001331127

pISSN:

2148-6905