Identification of Alkaliphilic Bacillus Species Isolated from Lake Van and Its Surroundings by Computerized Analysis of Extracellular Protein Profiles

İsmet BERBER; Elçin YENİDÜNYA

Makale detayları

Identification of Alkaliphilic Bacillus Species Isolated from Lake Van and Its Surroundings by Computerized Analysis of Extracellular Protein Profiles

Yazarlar:

İsmet BERBER | Elçin YENİDÜNYA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2005 Vol.29 Issue 3, pp.181-188

Belge Tipi:

Makale

Yayın Tarihi:

2/2005

Belge Dili:

Türkçe

Document ID (Org No.):

2000813478

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

Five reference Bacillus species and 17 native alkaliphilic Bacillus strains, isolated from the water of Lake Van and the soil surrounding it, were identified using phenotypic characteristics and extracellular protein profiles by the method of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). According to the phenotypic characteristics, all the native isolates were Gram-positive, aerobic, endospore-forming, motile, facultatively alkaliphilic Bacillus spp. It was also found that the native alkaliphilic Bacillus isolates were distinguished phenotypically from other Bacillus species.  A numerical analysis based on the resulting extracellular protein profiles revealed 5 distinct clusters: native isolates and B. megaterium formed 4 clusters that varied between 69% and 100% similarity, and the other reference Bacillus species formed a cluster with a similarity of above 70%. The research showed that extracellular protein profiles obtained by SDS-PAGE provide an effective approach to the investigation of taxonomic relationships within native alkaliphilic Bacillus isolates and other Bacillus species.

 

Five reference Bacillus species and 17 native alkaliphilic Bacillus strains, isolated from the water of Lake Van and the soil surrounding it, were identified using phenotypic characteristics and extracellular protein profiles by the method of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). According to the phenotypic characteristics, all the native isolates were Gram-positive, aerobic, endospore-forming, motile, facultatively alkaliphilic Bacillus spp. It was also found that the native alkaliphilic Bacillus isolates were distinguished phenotypically from other Bacillus species.  A numerical analysis based on the resulting extracellular protein profiles revealed 5 distinct clusters: native isolates and B. megaterium formed 4 clusters that varied between 69% and 100% similarity, and the other reference Bacillus species formed a cluster with a similarity of above 70%. The research showed that extracellular protein profiles obtained by SDS-PAGE provide an effective approach to the investigation of taxonomic relationships within native alkaliphilic Bacillus isolates and other Bacillus species.

Makale detayları

Identification of Alkaliphilic Bacillus Species Isolated from Lake Van and Its Surroundings by Computerized Analysis of Extracellular Protein Profiles

Yazarlar:

İsmet BERBER | Elçin YENİDÜNYA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2005 Vol.29 Issue 3, pp.181-188

Belge Tipi:

Makale

Yayın Tarihi:

2/2005

Belge Dili:

Türkçe

Document ID (Org No.):

2000813478

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Lake Van

identification

facultatively alkaliphilic Bacillus spp.

phenotypic characterization

SDS-PAGE

Sayı

Makale detayları

Identification of Alkaliphilic Bacillus Species Isolated from Lake Van and Its Surroundings by Computerized Analysis of Extracellular Protein Profiles

Yazarlar: İsmet BERBER | Elçin YENİDÜNYA

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2005 Vol.29 Issue 3, pp.181-188

Belge Tipi: Makale

Yayın Tarihi: 2/2005

Belge Dili: Türkçe

Document ID (Org No.): 2000813478

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Lake Van

identification

facultatively alkaliphilic Bacillus spp.

phenotypic characterization

SDS-PAGE

Sayı

Yazarlar:

İsmet BERBER | Elçin YENİDÜNYA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2005 Vol.29 Issue 3, pp.181-188

Belge Tipi:

Makale

Yayın Tarihi:

2/2005

Belge Dili:

Türkçe

Document ID (Org No.):

2000813478

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152