In vitro Anti-amoebic and Cytotoxic Activity of Rosa gallica and Picea orientalis Leaf Aqueous Extracts

Erdogan Malatyali; Ömer Erdoğan

Makale detayları

In vitro Anti-amoebic and Cytotoxic Activity of Rosa gallica and Picea orientalis Leaf Aqueous Extracts

Yazarlar:

Erdogan Malatyali | Ömer Erdoğan

Yayıncı:

Bingol University

Ana Başlıklar:

Hücre Gelişimi | Proliferasyon ve Ölümü

Dergi:

Türk Doğa ve Fen Dergisi Vol.14 Issue 2, pp.103-110

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001352632

pISSN:

2149-6366

Anahtar kelimeler:

Özet:

 Acanthamoeba spp. are free-living amoebae that can cause keratitis and granulomatous encephalitis in humans. This study aimed to evaluate the anti-amoebic and cytotoxic effects of aqueous leaf extracts of Rosa gallica and Picea orientalis on Acanthamoeba spp. and two cell lines. An environmental isolate of Acanthamoeba spp. was cultured on non-nutrient agar with Escherichia coli, and viability was tested with trypan blue method and microscopic observation of morphologies after exposure to extracts. Cytotoxicity was tested on SH-SY5Y human neuroblastoma and HaCaT human keratinocyte cell lines via the MTT assay. No viable trophozoites were observed after six hours of incubation with 30 mg mL-1 P. orientalis extract, whereas 20.7 of trophozoite viability was found in R. gallica extract with same concentration after 24 hours. Both extracts demonstrated limited efficacy against the encysted stage, with cysts remaining viable after 24 hours, over 70 viability. In addition, dose-dependent cytotoxicity was observed for both extracts on SH-SY5Y and HaCaT cells. This study provides the first in vitro evidence of the anti-amoebic activity of R. gallica and P. orientalis aqueous leaf extracts. However, their limited effect on cysts and cytotoxicity highlight the need for further in vivo studies to explore therapeutic potential.

 Acanthamoeba spp. are free-living amoebae that can cause keratitis and granulomatous encephalitis in humans. This study aimed to evaluate the anti-amoebic and cytotoxic effects of aqueous leaf extracts of Rosa gallica and Picea orientalis on Acanthamoeba spp. and two cell lines. An environmental isolate of Acanthamoeba spp. was cultured on non-nutrient agar with Escherichia coli, and viability was tested with trypan blue method and microscopic observation of morphologies after exposure to extracts. Cytotoxicity was tested on SH-SY5Y human neuroblastoma and HaCaT human keratinocyte cell lines via the MTT assay. No viable trophozoites were observed after six hours of incubation with 30 mg mL-1 P. orientalis extract, whereas 20.7 of trophozoite viability was found in R. gallica extract with same concentration after 24 hours. Both extracts demonstrated limited efficacy against the encysted stage, with cysts remaining viable after 24 hours, over 70 viability. In addition, dose-dependent cytotoxicity was observed for both extracts on SH-SY5Y and HaCaT cells. This study provides the first in vitro evidence of the anti-amoebic activity of R. gallica and P. orientalis aqueous leaf extracts. However, their limited effect on cysts and cytotoxicity highlight the need for further in vivo studies to explore therapeutic potential.

 Acanthamoeba spp. are free-living amoebae that can cause keratitis and granulomatous encephalitis in humans. This study aimed to evaluate the anti-amoebic and cytotoxic effects of aqueous leaf extracts of Rosa gallica and Picea orientalis on Acanthamoeba spp. and two cell lines. An environmental isolate of Acanthamoeba spp. was cultured on non-nutrient agar with Escherichia coli, and viability was tested with trypan blue method and microscopic observation of morphologies after exposure to extracts. Cytotoxicity was tested on SH-SY5Y human neuroblastoma and HaCaT human keratinocyte cell lines via the MTT assay. No viable trophozoites were observed after six hours of incubation with 30 mg mL-1 P. orientalis extract, whereas 20.7 of trophozoite viability was found in R. gallica extract with same concentration after 24 hours. Both extracts demonstrated limited efficacy against the encysted stage, with cysts remaining viable after 24 hours, over 70 viability. In addition, dose-dependent cytotoxicity was observed for both extracts on SH-SY5Y and HaCaT cells. This study provides the first in vitro evidence of the anti-amoebic activity of R. gallica and P. orientalis aqueous leaf extracts. However, their limited effect on cysts and cytotoxicity highlight the need for further in vivo studies to explore therapeutic potential.

 Acanthamoeba spp. potansiyel patojenik serbest yaşayan amipler arasında yer almakta olup insanlarda keratit ve granulomatöz ensefalite neden olabilmektedir. Bu çalışmada, Rosa gallica ve Picea orientalisin yaprak özütlerinin Acanthamoeba spp.ye karşı anti-amebik ve iki faklı hücre hattında sitotoksik aktivitelerinin araştırılması amaçlanmıştır. Çevresel örnekten izole edilen Acanthamoeba spp. izolatı, Escherichia coli ile kaplanmış besleyici değeri olmayan agar kültüründe çoğaltılmıştır. Farklı konsantrasyonlardaki özütler ile inkübe edilen parazitin canlılığı, trypan mavisi yöntemiyle ve morfolojik değişiklikler gözlemlenerek takip edilmiştir. Ayrıca, özütlerin sitotoksik aktiviteleri, SH-SY5Y insan nöroblastoma ve HaCaT insan keratinosit hücre hatlarında MTT yöntemiyle test edilmiştir. Altı saatlik 30 mg mL-1 P. orientalis özütü inkübasyonu sonrasında canlı trofozoite rastlanmazken, aynı konsantrasyonda R. gallica özütü ile 24 saatlik inkübasyon sonrası trofozoitlerin ortalama 20.7sinin canlı kaldığı gözlemlenmiştir. Trofozoit formunun aksine her iki özüt de, Acanthamoeba spp.nin kist formuna karşı sınırlı etki göstermiştir; kistler 24 saatlik inkübasyon sonrasında 70in üzerinde canlı kalmıştır. Ayrıca, her iki özüt de SH-SY5Y ve HaCaT hücrelerinde doz bağımlı sitotoksik etki göstermiştir. Bu in vitro çalışma sonucunda R. gallica ve P. orientalis yaprağı su özütlerinin anti-amebik aktivitesine dair ilk veriler elde edilmiştir. Özütlerin terapötik uygulama potansiyellerinin değerlendirilmesi için in-vivo çalışmalara ihtiyaç bulunmaktadır.

Makale detayları

In vitro Anti-amoebic and Cytotoxic Activity of Rosa gallica and Picea orientalis Leaf Aqueous Extracts

Yazarlar:

Erdogan Malatyali | Ömer Erdoğan

Yayıncı:

Bingol University

Ana Başlıklar:

Hücre Gelişimi | Proliferasyon ve Ölümü

Dergi:

Türk Doğa ve Fen Dergisi Vol.14 Issue 2, pp.103-110

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001352632

pISSN:

2149-6366

Acanthamoeba spp.

Rosa gallica

Picea orientalis

extract

cytotoxicityAcanthamoeba spp.

Rose gallica

Picea orientalis

özüt

sitotoksisite

Sayı

Makale detayları

Yazarlar: Erdogan Malatyali | Ömer Erdoğan

Yayıncı: Bingol University

Ana Başlıklar: Hücre Gelişimi | Proliferasyon ve Ölümü

Dergi: Türk Doğa ve Fen Dergisi Vol.14 Issue 2, pp.103-110

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: Türkçe

Document ID (Org No.): 2001352632

pISSN: 2149-6366

Sayı

Yazarlar:

Erdogan Malatyali | Ömer Erdoğan

Yayıncı:

Bingol University

Ana Başlıklar:

Hücre Gelişimi | Proliferasyon ve Ölümü

Dergi:

Türk Doğa ve Fen Dergisi Vol.14 Issue 2, pp.103-110

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001352632

pISSN:

2149-6366