Protective Effect of Bee Products Against Oxidative Damage in Erythrocytes

Orhan Değer; Ertuğrul Yiğit; Katip Korkmaz; Pınar Aygün; Ali Asghari; Kübra Akbulut Çakıroğlu; Selim Demir

Makale detayları

Protective Effect of Bee Products Against Oxidative Damage in Erythrocytes

Yazarlar:

Orhan Değer | Ertuğrul Yiğit | Katip Korkmaz | Pınar Aygün | Ali Asghari | Kübra Akbulut Çakıroğlu | Selim Demir

Yayıncı:

Gumushane University

Ana Başlıklar:

Sağlık Kurumları Yönetimi

Dergi:

Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi Vol.12 Issue 1, pp.167-174

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001285940

pISSN:

2146-9954

Anahtar kelimeler:

Özet:

 The positive effects of bee products on human health are due to their antioxidant composition. However, their possible protective efficacy against t-butylhydroxyperoxide t-BHP-induced oxidative damage on human erythrocytes has not been investigated. Our aim in this study is to investigate whether propolis, pollen and royal jelly have protective efficacy against t-BHP-induced oxidative damage in isolated erythrocytes. Propolis and pollen samples collected from various regions of Turkey were extracted by mixing with various solvents water and ethanol. Commercially purchased royal jelly was also extracted with water. The erythrocytes collected from 15 volunteers were centrifuged and packaged by washing with isotonic saline. All RBC packets were pooled together. The final erythrocyte packet was divided into seven groups as control, water extract of propolis, ethanolic extract of propolis, water extract of pollen, water extract of royal jelly, positive control quercetin and t-BHP groups. Erythrocytes were first treated with extracts of bee products and then t-BHP was added. Protective activities of bee products were investigated by malondialdehyde MDA, total oxidant capacity TOS, total antioxidant capacity TAS, superoxide dismutase SOD and catalase CAT activities. Statistically, one way ANOVA and post-hoc Tukey tests were applied. As a result of the study, it was found that all bee products contributed to keeping MDA levels close to the control group. p0.05. Again, while TOS levels of all bee products decreased compared to t-BHP, TAS levels increased p0.05. No significant effect of bee products on SOD and CAT enzyme activities was observed. p0.05.

 The positive effects of bee products on human health are due to their antioxidant composition. However, their possible protective efficacy against t-butylhydroxyperoxide t-BHP-induced oxidative damage on human erythrocytes has not been investigated. Our aim in this study is to investigate whether propolis, pollen and royal jelly have protective efficacy against t-BHP-induced oxidative damage in isolated erythrocytes. Propolis and pollen samples collected from various regions of Turkey were extracted by mixing with various solvents water and ethanol. Commercially purchased royal jelly was also extracted with water. The erythrocytes collected from 15 volunteers were centrifuged and packaged by washing with isotonic saline. All RBC packets were pooled together. The final erythrocyte packet was divided into seven groups as control, water extract of propolis, ethanolic extract of propolis, water extract of pollen, water extract of royal jelly, positive control quercetin and t-BHP groups. Erythrocytes were first treated with extracts of bee products and then t-BHP was added. Protective activities of bee products were investigated by malondialdehyde MDA, total oxidant capacity TOS, total antioxidant capacity TAS, superoxide dismutase SOD and catalase CAT activities. Statistically, one way ANOVA and post-hoc Tukey tests were applied. As a result of the study, it was found that all bee products contributed to keeping MDA levels close to the control group. p0.05. Again, while TOS levels of all bee products decreased compared to t-BHP, TAS levels increased p0.05. No significant effect of bee products on SOD and CAT enzyme activities was observed. p0.05.

 The positive effects of bee products on human health are due to their antioxidant composition. However, their possible protective efficacy against t-butylhydroxyperoxide t-BHP-induced oxidative damage on human erythrocytes has not been investigated. Our aim in this study is to investigate whether propolis, pollen and royal jelly have protective efficacy against t-BHP-induced oxidative damage in isolated erythrocytes. Propolis and pollen samples collected from various regions of Turkey were extracted by mixing with various solvents water and ethanol. Commercially purchased royal jelly was also extracted with water. The erythrocytes collected from 15 volunteers were centrifuged and packaged by washing with isotonic saline. All RBC packets were pooled together. The final erythrocyte packet was divided into seven groups as control, water extract of propolis, ethanolic extract of propolis, water extract of pollen, water extract of royal jelly, positive control quercetin and t-BHP groups. Erythrocytes were first treated with extracts of bee products and then t-BHP was added. Protective activities of bee products were investigated by malondialdehyde MDA, total oxidant capacity TOS, total antioxidant capacity TAS, superoxide dismutase SOD and catalase CAT activities. Statistically, one way ANOVA and post-hoc Tukey tests were applied. As a result of the study, it was found that all bee products contributed to keeping MDA levels close to the control group. p0.05. Again, while TOS levels of all bee products decreased compared to t-BHP, TAS levels increased p0.05. No significant effect of bee products on SOD and CAT enzyme activities was observed. p0.05.

 ÖZ Arı ürünlerinin insan sağlığı üzerindeki olumlu etkileri, antioksidan bileşimlerinden kaynaklan-maktadır. Ancak insan eritrositleri üzerinde t-butilhidroksiperoksit t-BHP kaynaklı oksidatif hasara karşı olası koruyucu etkinlikleri araştırılmamıştır. Bu çalışmadaki amacımız propolis, polen ve arı sütünün, izole edilen eritrositlerde t-BHP indüklü oksidatif hasara karşı koruyucu etkinliğe sahip olup olmadığını araştırmaktır. Türkiyenin çeşitli bölgelerinden toplanan propolis ve polen örnekleri çeşitli solventler su ve etanol ile karıştırılarak ekstrakte edildi. Ticari olarak satın alınan arı sütü de su ile ekstrakte edildi. 15 gönüllüden toplanılan eritrositler santrifüj edildi ve izotonik tuzla yıkanılarak paketlendi. Tüm RBC paketleri bir araya toplandı ve kontrol, sulu propolis ekstraktı, etanollü propolis ekstraktı, sulu polen ekstraktı, sulu arı sütü ekstraktı, pozitif kontrol kuersetin ve t-BHP ile olmak üzere yedi gruba ayrıldı. Eritrositler ilk olarak arı ürünlerinin ekstraktları ile muamele edilmiştir ve daha sonra t-BHP eklenmiştir. Arı ürünlerinin koruyucu etkinlikleri, malondialdehit MDA, toplam oksidan kapasite TOK, toplam antioksidan kapasite TAK, süperoksit dismutaz SOD ve katalaz KAT aktiviteleri ile araştırıldı. İstatistiksel olarak, ANOVA ve post-hoc Tukey testleri uygulandı. Çalışma sonucunda tüm arı ürünlerinin MDA seviyelerini kontrol grubuna yakın seviyelerde kalmasına katkı sağladığı bulunmuştur. p0.05. Yine tüm arı ürünlerinin t-BHP grubuna göre TOK düzeylerini düşerken TAK düzeyleri ise yükselmiştir p0.05. Arı ürünlerinin SOD ve KAT seviyeleri üzerinde önemli bir etkisi gözlenmedi p0.05.

Makale detayları

Protective Effect of Bee Products Against Oxidative Damage in Erythrocytes

Yazarlar:

Orhan Değer | Ertuğrul Yiğit | Katip Korkmaz | Pınar Aygün | Ali Asghari | Kübra Akbulut Çakıroğlu | Selim Demir

Yayıncı:

Gumushane University

Ana Başlıklar:

Sağlık Kurumları Yönetimi

Dergi:

Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi Vol.12 Issue 1, pp.167-174

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001285940

pISSN:

2146-9954

Antioxidants

Bee products

Erythrocyte

Oxidative stress

Antioksidan

Arı ürünleri

Eritrosit

Oksidatif stres

Sayı

Makale detayları

Yazarlar: Orhan Değer | Ertuğrul Yiğit | Katip Korkmaz | Pınar Aygün | Ali Asghari | Kübra Akbulut Çakıroğlu | Selim Demir

Yayıncı: Gumushane University

Ana Başlıklar: Sağlık Kurumları Yönetimi

Dergi: Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi Vol.12 Issue 1, pp.167-174

Belge Tipi: Makale

Yayın Tarihi: 3/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001285940

pISSN: 2146-9954

Sayı

Yazarlar:

Orhan Değer | Ertuğrul Yiğit | Katip Korkmaz | Pınar Aygün | Ali Asghari | Kübra Akbulut Çakıroğlu | Selim Demir

Yayıncı:

Gumushane University

Ana Başlıklar:

Sağlık Kurumları Yönetimi

Dergi:

Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi Vol.12 Issue 1, pp.167-174

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001285940

pISSN:

2146-9954