Investigation of the Cytotoxic Effects of Momordica Charantina Extract on Leishmania  Parasites with Amphotericin B and Miltefosine Drug Combinations

Kübra Kelleci; Murat Ihlamur; Melisa Özkan; Emrah Şefik Abamor

Makale detayları

Investigation of the Cytotoxic Effects of Momordica Charantina Extract on Leishmania Parasites with Amphotericin B and Miltefosine Drug Combinations

Yazarlar:

Kübra Kelleci | Murat Ihlamur | Melisa Özkan | Emrah Şefik Abamor

Yayıncı:

Cukurova University

Ana Başlıklar:

Biyomühendislik (Diğer)

Dergi:

Çukurova Üniversitesi Mühendislik Fakültesi Dergisi Vol.39 Issue 1, pp.1-7

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001314385

pISSN:

2757-9255

Anahtar kelimeler:

Özet:

 Leishmaniasis is a tropical disease caused by Leishmania parasites. It is known that L. infantum causes visceral leishmaniasis VL, which is the deadliest form of the disease, popularly known as kala-azar. Antileishmanial agents are already available in the market, but these chemotherapeutic agents are known to have toxicities and side effects. Interest in medicinal plants and their extracts is increasing day by day, with almost no side effects. In this study, bitter melon Momordica charantina extracts with rich chemical content and exhibiting anti-leishmanial properties were used to develop an effective drug formulation for leishmaniasis. In the study, the cytotoxic effect of bitter melon extracts alone or in combination with different antileishmanial drugs Amphotericin B and miltefosine was investigated in RAW 264.7 macrophage cells. In addition, the lethal activity of the obtained extract-drug formulations on L. infantum parasite cells was also investigated by MTT analysis method. According to the data obtained from the study, it was determined that the extract did not have a toxic effect alone, when combined with the drug, it reduced the toxic effect of the drug on the cells of the innate immune system, and increased the cytotoxic effect on the leishmania parasites. It is thought that the results obtained will help to develop a treatment approach against VL.

 Leishmaniasis is a tropical disease caused by Leishmania parasites. It is known that L. infantum causes visceral leishmaniasis VL, which is the deadliest form of the disease, popularly known as kala-azar. Antileishmanial agents are already available in the market, but these chemotherapeutic agents are known to have toxicities and side effects. Interest in medicinal plants and their extracts is increasing day by day, with almost no side effects. In this study, bitter melon Momordica charantina extracts with rich chemical content and exhibiting anti-leishmanial properties were used to develop an effective drug formulation for leishmaniasis. In the study, the cytotoxic effect of bitter melon extracts alone or in combination with different antileishmanial drugs Amphotericin B and miltefosine was investigated in RAW 264.7 macrophage cells. In addition, the lethal activity of the obtained extract-drug formulations on L. infantum parasite cells was also investigated by MTT analysis method. According to the data obtained from the study, it was determined that the extract did not have a toxic effect alone, when combined with the drug, it reduced the toxic effect of the drug on the cells of the innate immune system, and increased the cytotoxic effect on the leishmania parasites. It is thought that the results obtained will help to develop a treatment approach against VL.

 Leishmaniasis is a tropical disease caused by Leishmania parasites. It is known that L. infantum causes visceral leishmaniasis VL, which is the deadliest form of the disease, popularly known as kala-azar. Antileishmanial agents are already available in the market, but these chemotherapeutic agents are known to have toxicities and side effects. Interest in medicinal plants and their extracts is increasing day by day, with almost no side effects. In this study, bitter melon Momordica charantina extracts with rich chemical content and exhibiting anti-leishmanial properties were used to develop an effective drug formulation for leishmaniasis. In the study, the cytotoxic effect of bitter melon extracts alone or in combination with different antileishmanial drugs Amphotericin B and miltefosine was investigated in RAW 264.7 macrophage cells. In addition, the lethal activity of the obtained extract-drug formulations on L. infantum parasite cells was also investigated by MTT analysis method. According to the data obtained from the study, it was determined that the extract did not have a toxic effect alone, when combined with the drug, it reduced the toxic effect of the drug on the cells of the innate immune system, and increased the cytotoxic effect on the leishmania parasites. It is thought that the results obtained will help to develop a treatment approach against VL.

 Leishmaniasis, Leishmania parazitlerinin neden olduğu tropikal bir hastalıktır. Halk arasında kala-azar olarak bilinen, hastalığın en ölümcül formu olan viserial leishmaniasise VL L. infantumun neden olduğu bilinmektedir. Piyasada hali hazırda antileishmanial ajanlar mevcuttur, ancak bu kemoterapötik ajanların toksisitelerinin ve yan etkilerinin olduğu bilinmektedir. Şifalı bitkiler ve onların ekstraktlarına olan ilgi, yan etkilerinin yok denecek kadar az olmasıyla her geçen gün artmaktadır. Bu çalışmada leishmaniasise yönelik etkin bir ilaç formülasyonu geliştirmek için, zengin kimyasal içeriğe sahip, anti-leishmanial özellik sergileyen kudret narı Momordica charantina özütleri kullanılmıştır. Çalışmada kudret narı ekstraktları tek başına ve farklı antileishmanial ilaçlarla Amfoterisin B ve miltefosin kombine edilerek RAW 264.7 makrofaj hücrelerinde sitotoksik etkisi araştırılmıştır. Ayrıca elde edilen ekstrakt-ilaç formülasyonlarının L. infantum parazit hücreleri üzerindeki öldürücü etkinliği de MTT analiz yöntemi ile incelenmiştir. Çalışmadan elde edilen verilere göre ekstraktın tek başına toksik etkisinin olmadığı, ilaçla kombine edildiği zaman doğal immün sistem hücreleri üzerinde ilacın toksik etkisini azalttığı, leishmania parazitleri üzerinde ise sitotoksik etkisini arttırdığı belirlenmiştir. Elde edilen sonuçların VL karşı tedavi yaklaşımının geliştirilmesine yardımcı olacağı düşünülmektedir.

Makale detayları

Investigation of the Cytotoxic Effects of Momordica Charantina Extract on Leishmania Parasites with Amphotericin B and Miltefosine Drug Combinations

Yazarlar:

Kübra Kelleci | Murat Ihlamur | Melisa Özkan | Emrah Şefik Abamor

Yayıncı:

Cukurova University

Ana Başlıklar:

Biyomühendislik (Diğer)

Dergi:

Çukurova Üniversitesi Mühendislik Fakültesi Dergisi Vol.39 Issue 1, pp.1-7

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001314385

pISSN:

2757-9255

Leishmania infantum

Momordica charantina

Cytotoxicity

AntileishmanialLeishmania infantum

Momordica charantina

Sitotoksisite

Antileishmanial

Sayı

Makale detayları

Yazarlar: Kübra Kelleci | Murat Ihlamur | Melisa Özkan | Emrah Şefik Abamor

Yayıncı: Cukurova University

Ana Başlıklar: Biyomühendislik (Diğer)

Dergi: Çukurova Üniversitesi Mühendislik Fakültesi Dergisi Vol.39 Issue 1, pp.1-7

Belge Tipi: Makale

Yayın Tarihi: 3/2024

Belge Dili: Türkçe

Document ID (Org No.): 2001314385

pISSN: 2757-9255

Sayı

Yazarlar:

Kübra Kelleci | Murat Ihlamur | Melisa Özkan | Emrah Şefik Abamor

Yayıncı:

Cukurova University

Ana Başlıklar:

Biyomühendislik (Diğer)

Dergi:

Çukurova Üniversitesi Mühendislik Fakültesi Dergisi Vol.39 Issue 1, pp.1-7

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001314385

pISSN:

2757-9255