Effects of Nano Zinc Oxide-Enriched Diets on Bone Morphometry and Biomechanical Strength in Japanese Quails

Firuze Türker Yavaş; İsmail Gökçe Yıldırım; Figen Sevil Kilimci

Makale detayları

Effects of Nano Zinc Oxide-Enriched Diets on Bone Morphometry and Biomechanical Strength in Japanese Quails

Yazarlar:

Firuze Türker Yavaş | İsmail Gökçe Yıldırım | Figen Sevil Kilimci

Yayıncı:

Harran University

Ana Başlıklar:

Veteriner Anatomi ve Fizyoloji

Dergi:

Harran Üniversitesi Veteriner Fakültesi Dergisi Vol.14 Issue 1, pp.52-59

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001351031

pISSN:

2146-7188

Anahtar kelimeler:

Özet:

 Zinc is an essential trace element critical for numerous biological functions, including bone development, enzyme activity, and immune response. This study investigated the effects of zinc oxide ZnO and nano zinc oxide NZn supplementation on bone morphometry and biomechanical properties in Japanese quails Coturnix coturnix japonica. A total of 118 one-day-old quails were divided into three groups: control C, zinc oxide Zn, and nano zinc oxide NZn, with each group further subdivided into replicates. Diets were formulated to provide 75 mgkg zinc, with additional zinc sources added to achieve the desired levels. Morphometric and biomechanical analyses were conducted using a 3-point bending test to evaluate tibiotarsus bone strength and structural properties. Results indicated that the NC group exhibited a significantly higher external mediolateral diameter ExtMLD compared to the Zn and C groups P  0.003, suggesting enhanced periosteal bone growth with NZn supplementation. However, no significant differences were observed in internal diameters or biomechanical parameters such as breaking force, moment of inertia, strength, stiffness, and elastic modulus among the groups P0.05. Sex-based comparisons revealed that female quails in the Zn and C groups had significantly higher breaking force and moment of inertia compared to males P  0.05. Still, no such differences were observed in the NZn group. These findings suggest that while NZn may positively influence specific morphometric parameters, its impact on biomechanical strength remains limited. The study highlights the need for further research to elucidate the mechanisms underlying zincs effects on bone development.

 Zinc is an essential trace element critical for numerous biological functions, including bone development, enzyme activity, and immune response. This study investigated the effects of zinc oxide ZnO and nano zinc oxide NZn supplementation on bone morphometry and biomechanical properties in Japanese quails Coturnix coturnix japonica. A total of 118 one-day-old quails were divided into three groups: control C, zinc oxide Zn, and nano zinc oxide NZn, with each group further subdivided into replicates. Diets were formulated to provide 75 mgkg zinc, with additional zinc sources added to achieve the desired levels. Morphometric and biomechanical analyses were conducted using a 3-point bending test to evaluate tibiotarsus bone strength and structural properties. Results indicated that the NC group exhibited a significantly higher external mediolateral diameter ExtMLD compared to the Zn and C groups P  0.003, suggesting enhanced periosteal bone growth with NZn supplementation. However, no significant differences were observed in internal diameters or biomechanical parameters such as breaking force, moment of inertia, strength, stiffness, and elastic modulus among the groups P0.05. Sex-based comparisons revealed that female quails in the Zn and C groups had significantly higher breaking force and moment of inertia compared to males P  0.05. Still, no such differences were observed in the NZn group. These findings suggest that while NZn may positively influence specific morphometric parameters, its impact on biomechanical strength remains limited. The study highlights the need for further research to elucidate the mechanisms underlying zincs effects on bone development.

 Zinc is an essential trace element critical for numerous biological functions, including bone development, enzyme activity, and immune response. This study investigated the effects of zinc oxide ZnO and nano zinc oxide NZn supplementation on bone morphometry and biomechanical properties in Japanese quails Coturnix coturnix japonica. A total of 118 one-day-old quails were divided into three groups: control C, zinc oxide Zn, and nano zinc oxide NZn, with each group further subdivided into replicates. Diets were formulated to provide 75 mgkg zinc, with additional zinc sources added to achieve the desired levels. Morphometric and biomechanical analyses were conducted using a 3-point bending test to evaluate tibiotarsus bone strength and structural properties. Results indicated that the NC group exhibited a significantly higher external mediolateral diameter ExtMLD compared to the Zn and C groups P  0.003, suggesting enhanced periosteal bone growth with NZn supplementation. However, no significant differences were observed in internal diameters or biomechanical parameters such as breaking force, moment of inertia, strength, stiffness, and elastic modulus among the groups P0.05. Sex-based comparisons revealed that female quails in the Zn and C groups had significantly higher breaking force and moment of inertia compared to males P  0.05. Still, no such differences were observed in the NZn group. These findings suggest that while NZn may positively influence specific morphometric parameters, its impact on biomechanical strength remains limited. The study highlights the need for further research to elucidate the mechanisms underlying zincs effects on bone development.

 Çinko, kemik gelişimi, enzim aktivitesi ve bağışıklık yanıtı da dahil olmak üzere birçok biyolojik fonksiyon için kritik olan temel bir eser elementtir. Bu çalışma, çinko oksit ZnO ve nano çinko oksit NZn takviyesinin Japon bıldırcınlarında Coturnix coturnix japonica tibiotarsus kemiğinin morfometrisi ve biyomekanik özellikleri üzerindeki etkilerini araştırmıştır. Toplam 118 adet bir günlük bıldırcın, kontrol C, çinko oksit Zn ve nano çinko oksit NZn olmak üzere üç gruba ayrılmış ve her grup kendi içinde alt gruplara bölünmüştür. Diyetler, 75 mgkg çinko sağlayacak şekilde formüle edilmiş ve belirlenen düzeylere ulaşmak için ek çinko kaynakları kullanılmıştır. Morfometrik ve biyomekanik analizler, kemik dayanıklılığı ve yapısal özellikleri değerlendirmek amacıyla üç nokta eğme testi kullanılarak gerçekleştirilmiştir. Sonuçlar, NZn grubunun dış mediolateral çapının ExtMLD Zn ve C gruplarına kıyasla anlamlı derecede daha yüksek olduğunu P  0,003 göstermiştir, bu da NZn takviyesinin periosteal kemik büyümesini artırabileceğini düşündürmektedir. Ancak, gruplar arasında iç çaplar veya kırılma kuvveti, atalet momenti, mukavemet, rijitlik ve elastik modül gibi biyomekanik parametrelerde anlamlı bir farklılık gözlenmemiştir P  0,05. Cinsiyete bağlı karşılaştırmalarda, Zn ve C gruplarındaki dişi bıldırcınların kırılma kuvveti ve atalet momenti değerlerinin erkeklere kıyasla anlamlı derecede daha yüksek olduğu tespit edilmiştir P  0,05, ancak NZn grubunda benzer bir fark bulunmamıştır. Bu bulgular, NZnnun belirli morfometrik parametreler üzerinde olumlu etkiler gösterebileceğini ancak biyomekanik dayanıklılık üzerindeki etkisinin sınırlı olduğunu ortaya koymaktadır. Çalışma, çinkonun kemik gelişimi üzerindeki etkilerini açıklığa kavuşturmak amacıyla daha ileri araştırmalara ihtiyaç olduğunu vurgulamaktadır.

Makale detayları

Effects of Nano Zinc Oxide-Enriched Diets on Bone Morphometry and Biomechanical Strength in Japanese Quails

Yazarlar:

Firuze Türker Yavaş | İsmail Gökçe Yıldırım | Figen Sevil Kilimci

Yayıncı:

Harran University

Ana Başlıklar:

Veteriner Anatomi ve Fizyoloji

Dergi:

Harran Üniversitesi Veteriner Fakültesi Dergisi Vol.14 Issue 1, pp.52-59

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001351031

pISSN:

2146-7188

Biomechanic

bone morphometry

Japanese quails

zinc.Biyomekanik

çinko

Japon bıldırcını

kemik morfometrisi.

Sayı

Makale detayları

Yazarlar: Firuze Türker Yavaş | İsmail Gökçe Yıldırım | Figen Sevil Kilimci

Yayıncı: Harran University

Ana Başlıklar: Veteriner Anatomi ve Fizyoloji

Dergi: Harran Üniversitesi Veteriner Fakültesi Dergisi Vol.14 Issue 1, pp.52-59

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001351031

pISSN: 2146-7188

Sayı

Yazarlar:

Firuze Türker Yavaş | İsmail Gökçe Yıldırım | Figen Sevil Kilimci

Yayıncı:

Harran University

Ana Başlıklar:

Veteriner Anatomi ve Fizyoloji

Dergi:

Harran Üniversitesi Veteriner Fakültesi Dergisi Vol.14 Issue 1, pp.52-59

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001351031

pISSN:

2146-7188