Recombinant outer membrane protein Q and putative lipoprotein from  <i>Bordetella pertussis</i>  inducing strong humoral response were not protective alone in the  murine lung colonization model

Çiğdem YILMAZ; Erkan ÖZCENGİZ; Gülay ÖZCENGİZ

Makale detayları

Recombinant outer membrane protein Q and putative lipoprotein from <i>Bordetella pertussis</i> inducing strong humoral response were not protective alone in the murine lung colonization model

Yazarlar:

Çiğdem YILMAZ | Erkan ÖZCENGİZ | Gülay ÖZCENGİZ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2018 Vol.42 Issue 2, pp.123-131

Belge Tipi:

Makale

Yayın Tarihi:

4/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000811239

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Özet:

 

Despite  high  vaccination  coverage  after  introduction  of  whole  cell  (wP)  and  acellular  pertussis  (aP)  vaccines,  pertussis  
resurgence has been reported in many countries. aP vaccines are commonly preferred due to side effects of wP vaccines and formulated 
with  aluminum  hydroxide  (Alum),  which  is  not  an  effective  adjuvant  to  eliminate  Bordetella pertussis.  Low  efficiency  of  current  aP  
vaccines is thought to be the main reason for the resurgence for which newer generation aP vaccines are needed. In the present study, 
immunogenicity and protective efficacy of outer membrane protein Q (OmpQ) and a putative lipoprotein (Lpp) from 
B. pertussis were 
investigated in mice by using two different adjuvants, monophosphoryl lipid A (MPLA) or Alum. OmpQ and putative Lpp were cloned, 
expressed, and purified from
 Escherichia coli. 
The proteins were formulated to immunize mice. Both recombinant OmpQ and putative 
Lpp induced a significant increase in immunoglobulin G1 (IgG1) and immunoglobulin G2a (IgG2a) responses compared to the control 
group.  Moreover,  MPLA-adjuvanted  formulations  resulted  in  higher  IgG2a  levels  than  Alum-adjuvanted  ones.  However,  there  were  
no significant differences between test and control groups regarding interferon-gamma (IFN-γ) levels, and the mice lung colonization 
experiments indicated that neither rOmpQ nor rLpp could confer protection alone against 
B
. 
pertussis
 challenge.

Makale detayları

Recombinant outer membrane protein Q and putative lipoprotein from <i>Bordetella pertussis</i> inducing strong humoral response were not protective alone in the murine lung colonization model

Yazarlar: Çiğdem YILMAZ | Erkan ÖZCENGİZ | Gülay ÖZCENGİZ

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2018 Vol.42 Issue 2, pp.123-131

Belge Tipi: Makale

Yayın Tarihi: 4/2018

Belge Dili: İngilizce

Document ID (Org No.): 2000811239

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Sayı

Yazarlar:

Çiğdem YILMAZ | Erkan ÖZCENGİZ | Gülay ÖZCENGİZ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2018 Vol.42 Issue 2, pp.123-131

Belge Tipi:

Makale

Yayın Tarihi:

4/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000811239

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152